Adventitious bud induction method of macadimia nut

A bud induction and nut technology, applied in the field of macadamia adventitious bud induction, can solve the problems of complicated steps and long cycle, and achieve the effects of promoting cell division and expansion, reducing the pollution rate and overcoming adverse effects.

Active Publication Date: 2019-03-22
SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Another example is the document "Research on Tissue Culture of Macadamia Nuts_Xiao Zaiyun" which discloses taking HAES800 scion shoots and cutting them into stems with axillary or terminal buds. %HgCl 2 Soak in medium for 8 minutes, then rinse with sterile water for 5 times, culture the stem section of HAES800 in No. (1) stem section medium for 30 days, then transfer to (2) No. stem section medium to continue culturing, until 25 days, the more Injury t

Method used

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  • Adventitious bud induction method of macadimia nut
  • Adventitious bud induction method of macadimia nut
  • Adventitious bud induction method of macadimia nut

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Embodiment 1

[0030] A method for inducing adventitious buds of macadamia nuts, comprising the following steps:

[0031] 1) Macadamia nuts were used as the test material, and tender shoots at the early stage of extraction were used as explants, and the explants were hydroponically grown at a temperature of 25°C for 24 hours;

[0032] 2) Rinse the twigs in step 1) with water, transfer them to an ultra-clean workbench, put them into a sterilized empty culture bottle, soak them in TWEEN-20, disinfect them with alcohol, soak them in mercuric chloride, wash them with sterile water, and then put them in Blot the surface moisture on sterilized filter paper for later use;

[0033] 3) Step 2) After the treatment is completed, inoculate on the induction medium for light culture;

[0034] 4) inoculating the induced adventitious buds on the proliferation medium for proliferation culture.

[0035] In the above method, step 2) rinses with reclaimed water for 0.5 hours, soaks in TWEEN-20 for 3 minutes, ...

Embodiment 2

[0042] A method for inducing adventitious buds of macadamia nuts, comprising the following steps:

[0043] 1) Macadamia nuts were used as the test material, and tender shoots at the early stage of extraction were taken as explants, and the explants were hydroponically grown at a temperature of 30°C for 15 hours;

[0044] 2) Rinse the twigs in step 1) with water, transfer them to an ultra-clean workbench, put them into a sterilized empty culture bottle, soak them in TWEEN-20, disinfect them with alcohol, soak them in mercuric chloride, wash them with sterile water, and then put them in Blot the surface moisture on sterilized filter paper for later use;

[0045] 3) Step 2) After the treatment is completed, inoculate on the induction medium for light culture;

[0046] 4) inoculating the induced adventitious buds on the proliferation medium for proliferation culture.

[0047] In the above method, step 2) rinses in water for 0.6 hours, soaks in TWEEN-20 for 4 minutes, disinfects wi...

Embodiment 3

[0054] A method for inducing adventitious buds of macadamia nuts, comprising the following steps:

[0055] 1) Macadamia nuts were used as the test material, and tender shoots at the early stage of extraction were taken as explants, and the explants were hydroponically grown at a temperature of 20°C for 15 hours;

[0056] 2) Rinse the twigs in step 1) with water, transfer them to an ultra-clean workbench, put them into a sterilized empty culture bottle, soak them in TWEEN-20, disinfect them with alcohol, soak them in mercuric chloride, wash them with sterile water, and then put them in Blot the surface moisture on sterilized filter paper for later use;

[0057] 3) Step 2) After the treatment is completed, inoculate on the induction medium for light culture;

[0058] 4) inoculating the induced adventitious buds on the proliferation medium for proliferation culture.

[0059] In the above method, step 2) rinses in water for 0.3 hours, soaks in TWEEN-20 for 2 minutes, disinfects ...

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Abstract

The invention relates to the technical field of cultivation of macadimia nut, in particular to an adventitious bud induction method of macadimia nut. The method comprises the steps of using the macadimia nut as a tested material, carrying out hydroponic culture on newly sprouted tender branches used as explants, then washing the explants with water, transferring into an ultra-clean work table, soaking in Tween-20, disinfecting with alcohol, soaking in mercuric chloride, and washing with sterile water; after that, placing on sterilized filter paper for absorbing the surface water for later use;then, inoculating in an induction medium for carrying out lighting culture, and then inoculating a proliferation culture medium with the induced adventitious buds for carrying out proliferation culture. The method is used for carrying out macadimia nut adventitious bud induction under the in vitro condition, thus not only simplifying the disinfection step, but also obtaining vigorous and sterileadventitious buds.

Description

technical field [0001] The invention relates to the technical field of macadamia nut cultivation, in particular to a method for inducing adventitious buds of macadamia nuts. Background technique [0002] Macadamia ternifolia F.Muell is a tree nut native to Australia, belonging to the genus Macadamia ternifolia, also known as macadamia and Quinlan fruit. Because its nuts are rich in protein, vitamins and unsaturated fatty acids, it enjoys the reputation of "the king of dried fruits". As an evergreen tree, macadamia nut has good ecological and economic benefits. At present, tissue culture rapid propagation technology has been widely used in woody fruit trees. [0003] In terms of rapid propagation of macadamia nuts, researchers use the stem segments of macadamia nuts for tissue culture. For example, Patent No. 201510868889.X discloses a method for rapid propagation of macadamia nuts through tissue culture, including: cutting a 4cm long macadamia nut from an adult tree contai...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刘荣范建新刘小翠王代谷何凤平
Owner SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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