Method for rapidly identifying bacteria and fungi by utilizing Raman spectra

A technology of Raman spectroscopy and Raman spectroscopy, applied in the field of microbial identification, can solve the problems of high detection cost, high laboratory requirements, and inability to meet detection conditions, and achieve the effects of ensuring accuracy, simple operation, and shortening detection time.

Pending Publication Date: 2020-09-04
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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However, these two methods are costly and have high requirements for

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  • Method for rapidly identifying bacteria and fungi by utilizing Raman spectra
  • Method for rapidly identifying bacteria and fungi by utilizing Raman spectra
  • Method for rapidly identifying bacteria and fungi by utilizing Raman spectra

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Embodiment 1

[0060] Five clinical isolates of fungi (3 strains of Candida albicans, 3 strains of Candida krusei, 3 strains of Candida parapsilosis, 3 strains of Candida glabrata, 3 strains of Tropical Candida (Candida tropicalis, a total of 15 strains) and 7 clinically isolated bacteria (2 strains of Escherichia coli, 1 strain of Klebsiella pneumoniae, 1 strain of Pseudomonas aeruginosa (Pseudomonas aeruginosa), 1 strain of Acinetobacter baumannii, 2 strains of Staphylococcus aureus, 1 strain of Enterococcus faecalis, 1 strain of Staphylococcus epidermidis, 9 strains in total ), all strains were from the Institute of Antibiotics, Huashan Hospital Affiliated to Fudan University. Take 1ml of pure culture solution from each strain, centrifuge at 7000rpm for 2min, discard the supernatant, wash the precipitate three times with deionized water, take 2μL of the washed solution and spot it on an aluminum-coated glass slide, and air-dry it at room temperature. Raman detection.

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Abstract

The invention relates to a method for quickly identifying bacteria and fungi by using Raman spectra, which comprises the following steps: pretreating a sample to be identified, collecting the Raman spectra of the treated sample, removing the background of the collected Raman spectra and performing normalizing treatment, and identifying bacteria and fungi by using the treated spectra. Compared withthe prior art, the method is simple to operate, and complex sample pretreatment is not needed before Raman testing. According to the method, the amount of bacteria required for testing is small, enrichment culture is not needed, the detection time can be greatly shortened, and an identification result can be quickly given. By utilizing the characteristic that Raman spectra of cells can reflect chemical components of the cells, bacteria and fungi are accurately identified by comparing specific peak positions of the Raman spectra of the bacteria and the fungi, and errors caused by observing themorphological sizes of the cells by naked eyes and subjectively judging can be eliminated.

Description

technical field [0001] The invention belongs to the technical field of microorganism identification, in particular to a method for quickly identifying bacteria and fungi by using Raman spectrum. Background technique [0002] The identification of bacteria and fungi has very important clinical significance. Early identification of bacterial and fungal infections can help clinicians formulate targeted treatment plans, provide reference for clinical selection of effective antibacterial drugs, and reduce inappropriate use of broad-spectrum antibacterial drugs , while reducing the financial burden on patients. [0003] Commonly used identification methods for bacteria and fungi mainly include staining, biochemical methods, immunology, and molecular biology detection methods. The staining method clinically used to distinguish bacteria and fungi is mainly Gram staining. After staining, the fungi are Gram-positive and appear dark purple, with a size of about 10-100 μm; Gram-positiv...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28G06K9/62G06N20/10G06N3/04G06N3/08
CPCG01N21/65G01N1/28G06N20/10G06N3/08G06N3/045G06F18/2135G06F18/2411G06F18/241
Inventor 徐晓刚王明贵衣晓飞
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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