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Method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water

A technology for repairing plants and organotin, which is applied in the field of environmental bioengineering, can solve the problems of long adaptation period of organotin toxicity, unsatisfactory performance of degrading bacteria, single degrading enzyme, etc., achieve superior effect, accelerate detoxification and complete degradation, and process efficiency high effect

Active Publication Date: 2013-12-18
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the performance of degrading bacteria selected by researchers at home and abroad is not ideal; moreover, when a single degrading bacteria treats organotin in water, there are disadvantages such as easy loss, single degrading enzyme, and long adaptation period to organotin toxicity.

Method used

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  • Method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water
  • Method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water
  • Method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Joint treatment of organotin-polluted river water bodies by organotin-degrading bacteria and restoration plants

[0038](1) Pick the organotin-degrading bacteria Bacillus thuringiensis (Bacillusthuringiensis, from the Lianjiang sediments, Guiyu Town, Guangdong Province, from the slant of the organotin-degrading bacteria. The same below.) and inoculate it into the seed medium (seed medium) at 25 mg / L Components: beef extract 3g / L, peptone 10g / L, NaCl 5g / L.), in a 50r / min shaker for 10h, the organic tin degradation bacteria liquid was obtained; take the organic tin degradation bacteria liquid by 1% The volume ratios were respectively inoculated in the medium prepared according to the ingredients in Table 1, and placed in a fermenter for cultivation, and the effect of the medium components on the culture of the bacteria was investigated. When the bacterial density of the organotin-degrading bacteria reaches 5g / L, the bacterial cells are harvested by centrifugati...

Embodiment 2

[0050] Example 2: Joint treatment of organotin-contaminated wharf sewage by organotin-degrading bacteria and restoration plants

[0051] (1) Pick the organotin-degrading bacteria Bacillus thuringiensis from the slope of the organotin-degrading bacteria and inoculate it into the seed medium at 1 mg / L (seed medium composition: beef extract 10g / L, peptone 2g / L, NaCl 0g / L.), in a 100r / min shaker for 15h, the organotin-degrading bacteria solution was obtained; the organotin-degrading bacteria solution was inoculated in the culture medium at a volume ratio of 5% (medium composition: beef extract 12g / L, peptone 3g / L, NaCl 0g / L.), placed in a fermenter for cultivation, when the bacterial density of organotin-degrading bacteria reached 9g / L, the bacterial cells were harvested by centrifugation at 3000rpm, dried at 100°C, and obtained Bacillus inoculum;

[0052] The cultivation is carried out at a dissolved oxygen percentage of 80wt%, pH 5.8, and a temperature of 30°C;

[0053] (2) ...

Embodiment 3

[0057] Example 3: Joint treatment of organotin-contaminated seabed mud by organotin-degrading bacteria and restoration plants

[0058] (1) Pick the organotin-degrading bacteria Bacillus thuringiensis from the slope of the organotin-degrading bacteria and inoculate it into the seed medium at 50 mg / L (seed medium composition: beef extract 6g / L, peptone 5g / L, NaCl 8g / L.), after shaking and culturing in a shaker at 200r / min for 24h, the organotin-degrading bacterial liquid was obtained; the organotin-degrading bacterial liquid was inoculated in the culture medium at a volume ratio of 10% (medium composition: beef extract 8g / L, peptone 8g / L, NaCl 8g / L.), placed in a fermenter for cultivation, when the bacterial density of organotin-degrading bacteria reached 12g / L, the bacterial cells were harvested by centrifugation at 3000rpm, dried at 60°C, and obtained Bacillus inoculum;

[0059] The cultivation is carried out at a dissolved oxygen percentage of 80wt%, pH 5.8, and a temperat...

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Abstract

The invention discloses a method for using organic tin-degrading bacteria agent and remediation plants to treat organic tin polluted water. According to the invention, a large amount of organic tin-degrading bacteria are obtained through fermentation technology, and the bacteria are dried to obtain active bacteria agent which can be stored for a long time. Under the condition of planting remediation plants, the organic tin-degrading bacteria are added to remove organic tin by using the combined processing technology of organic tin-degrading bacteria agent and remediation plants. The method has the advantages that the combined organic tin pollutant processing technology of organic tin-degrading bacteria agent and remediation plants is adopted, the treatment efficiency is high, the cost is low, the method does not cause secondary pollution, the technical effect of the method is better than single microbial purifying, the quantity of the organic tin-degrading bacteria required to be added into water is small, and the remediation plants also has the feature of landscaping.

Description

technical field [0001] The invention belongs to the technical field of environmental bioengineering, and particularly relates to a method for treating organotin-polluted water bodies by utilizing organotin-degrading bacterial agents and repairing plants. Background technique [0002] Organotins are widely used as catalysts, insecticides, fungicides, herbicides, rust inhibitors, marine coatings, wood preservatives, textile antifungal agents and polymer material stabilizers, etc., and are the most widely used and the largest emissions One of the organic metals in China, with an annual usage of more than 100,000 tons. Organotin pollutants combine the characteristics of heavy metals and persistent organic pollutants, are highly toxic, persistent, and highly bioaccumulative, and can cause sexual distortions in species including molluscs, fish, and mammals, and It will endanger the ecosystem through the enrichment of the food chain. In the long-term production and use process, o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/32C02F3/34
CPCY02A20/402Y02W10/10
Inventor 叶锦韶尹华何宝燕彭辉张娜
Owner JINAN UNIVERSITY
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