Medium capable of improving survival rate of lactic acid bacteria during preservation and application thereof

Abstract

The invention discloses a medium capable of improving survival rate of lactic acid bacteria during preservation and application thereof. In the medium capable of improving the survival rate of the lactic acid bacteria during preservation provided by the invention, a carbon source in the lactic acid bacterium liquid medium is replaced from glucose to sucrose; and the medium can further contain calcium carbonate. According to the medium capable of improving the survival rate of the lactic acid bacteria during preservation, the carbon source of the medium is changed with lactic acid produced in neutralizing-culturing process of salt substances added, so that the pH value of the medium is increased; and thus, cold-storage preservation rate of the lactic acid bacteria is improved. Through comparative studies, it is found that the modified MRS medium, in which glucose is replaced by sucrose, has no adverse effects on culture effects of lactic acid bacteria which can be cultured by using thesucrose; however, the modified MRS medium is capable of significantly reducing mortality of the lactic acid bacteria in cold storage. During culture process, better preservation effects can be achieved by adding 1% of calcium carbonate into the medium. The medium utilizes the sucrose as the carbon source, so that the medium is low in cost and easy to use. In addition, the calcium carbonate is a neutralizing salt of acids; and the calcium carbonate is small in dosage and low in cost.

Description

technical field [0001] The invention belongs to the field of microorganism preservation, in particular to a medium for improving the survival rate of lactic acid bacteria preservation and its application. Background technique [0002] Lactic acid bacteria are widely used as a starter for fermented food, yogurt, silage, etc., so their low-cost cultivation and long-term storage are particularly important. So far, its standard medium uses glucose as the carbon source, and the number of bacteria in refrigerated storage decreases rapidly after cultivation, and long-term storage affects its use effect. One way to improve is to carry out vacuum freeze-drying of cultured lactic acid bacteria. Although this method has the advantages of high number of viable bacteria, long shelf life, and stable quality, freeze-drying requires expensive freeze-drying equipment and cumbersome freeze-drying procedures. Production users who do not have the conditions are difficult to achieve. Contents...

AI Technical Summary

Problems solved by technology

So far, its standard medium uses glucose as the carbon source, and the number of bacteria in refrigerated storage after cultivation decreases rapidly, and long-term storage affects its use effect

One way to improve is to carry out vacuum freeze-drying of cultured lactic acid bacteria. Although this method has the advantages of high number of viable bacteria, long shelf life, and stable quality, freeze-drying requires expensive freeze-drying equipment and cumbersome freeze-drying procedures. It is difficult for production users who do not have the conditions to realize

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