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Rhizobium japonicum culture medium and method for preparing liquid rhizobium japonicum agent by adopting rhizobium japonicum culture medium

A technology for soybean rhizobia and rhizobia agent, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of unbalanced nutrition, low bacterial activity, and the inability of soybean rhizobia to survive stably.

Active Publication Date: 2013-06-05
黑龙江省华龙生物科技有限公司
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Problems solved by technology

[0014] The purpose of the present invention is to solve the problem that the existing soybean liquid rhizobia agent is unbalanced in nutrition, the activity of the thalline is low and the soybean rhizobia prepared by fermentation process cannot survive stably at room temperature, and provide soybean rhizobia culture medium and adopt Its method for preparing soybean liquid rhizobia agent

Method used

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  • Rhizobium japonicum culture medium and method for preparing liquid rhizobium japonicum agent by adopting rhizobium japonicum culture medium
  • Rhizobium japonicum culture medium and method for preparing liquid rhizobium japonicum agent by adopting rhizobium japonicum culture medium
  • Rhizobium japonicum culture medium and method for preparing liquid rhizobium japonicum agent by adopting rhizobium japonicum culture medium

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specific Embodiment approach 1

[0022] Specific embodiment one: the soybean rhizobia medium of the present embodiment is composed of glycerol, mannitol, glucose, rhamnose, fructose, α-ketoglutarate, yeast powder, serine, arginine, NH 4 Cl, K 2 HPO 4 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, FeSO 4 ·7H 2 O, NaCl, Na 2 MoO 4 2H 2 An aqueous solution prepared from O and biotin; wherein, the concentration of glycerol is 1-3 g / L, the concentration of mannitol is 1-3 g / L, the concentration of glucose is 1-3 g / L, and the concentration of rhamnose is 1~3 g / L, fructose concentration 1~3 g / L, α-ketoglutarate concentration 0.5~1.5 g / L, yeast powder concentration 2.5~4.5 g / L, serine concentration 0.13~0.25 g / L, the concentration of arginine is 0.1~0.2 g / L, NH 4 Cl concentration is 0.65~1.6 g / L, K 2 HPO 4 The concentration is 1~3 g / L, KH 2 PO 4 The concentration is 0.2~0.5 g / L, MgSO 4 ·7H 2 O concentration is 0.1~0.3 g / L, CaCl 2 2H 2 O concentration is 0.1~0.2 g / L, FeSO 4 ·7H 2 O concentration i...

specific Embodiment approach 2

[0024] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the concentration of glycerol is 1.5-2.5 g / liter, the concentration of mannitol is 1.5-2.5 g / liter, and the concentration of glucose is 1.5-2.5 grams / L, the concentration of rhamnose is 1.5~2.5 g / L, the concentration of fructose is 1.5~2.5 g / L, the concentration of α-ketoglutaric acid is 0.75~1.25 g / L, the concentration of yeast powder is 3.0~4.0 g / L , the concentration of serine is 0.15~0.20 g / L, the concentration of arginine is 0.125~0.175 g / L, NH 4 Cl concentration is 0.8~1.25 g / L, K 2 HPO 4 The concentration is 1.1~1.6 g / L, KH 2 PO 4 The concentration is 0.25~0.4 g / L, MgSO 4 ·7H 2 O concentration is 0.15~0.25 g / L, CaCl 2 2H 2 O concentration is 0.11~0.15 g / L, FeSO 4 ·7H 2 O concentration is 0.006~0.009 g / L, NaCl concentration is 0.08~0.12 g / L, Na 2 MoO 4 2H 2 The O concentration is 0.015~0.025 g / L and the biotin concentration is 0.0008~0.0012 g / L. Others...

specific Embodiment approach 3

[0025] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the concentration of glycerol is 2.0 g / liter, the concentration of mannitol is 2.0 g / liter, the concentration of glucose is 2.0 g / liter, and the The concentration of plum sugar is 2.0 g / L, the concentration of fructose is 2.0 g / L, the concentration of α-ketoglutaric acid is 1.0 g / L, the concentration of yeast powder is 3.5 g / L, the concentration of serine is 0.18 g / L, arginine The concentration is 0.15 g / L, NH 4 Cl concentration is 1.1 g / L, K 2 HPO 4 The concentration is 1.2 g / L, KH 2 PO 4 The concentration is 0.34 g / L, MgSO 4 ·7H 2 O concentration is 0.2 g / L, CaCl 2 2H 2 O concentration is 0.13 g / L, FeSO 4 ·7H 2 O concentration is 0.0083 g / L, NaCl concentration is 0.1 g / L, Na 2 MoO 4 2H 2 The O concentration was 0.02 g / L and the biotin concentration was 0.001 g / L. Others are the same as in the first or second embodiment.

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Abstract

The invention relates a rhizobium japonicum culture medium and a method for preparing a liquid rhizobium japonicum agent by adopting the rhizobium japonicum culture medium and aims at solving the problem that an existing liquid rhizobium japonicum agent is unbalanced in nutrition and low in thallus activity and rhizobium japonicum prepared by means of a fermentation technology cannot stably survive at the room temperature. The rhizobium japonicum culture medium is an aqueous solution prepared by glycerin, mannitol, glucose, rhamnose, fructose, alpha-oxoglutarate, yeast powder, serine, arginine, NH4C1, K2HPO4, KH2PO4, MgSO4*7H2O, CaCl2*2H2O, FeSO4*7H2O, NaCl, Na2MoO4*2H2O and biotin, and can be obtained by controlling fermentation tank conditions. The viable count of microbial inoculum of the rhizobium japonicum culture medium preserved at 20 DEG C for 12 months can still reach to more than 100 hundred millions per milliliter.

Description

technical field [0001] The invention relates to a rhizobia culture medium and a method for preparing soybean rhizobia agent by using it. Background technique [0002] China's arable land area accounts for 7% of the world's arable land area, but nitrogen fertilizer consumption accounts for 25% of the global total, which is 3.75 times the world's average. The production of chemical synthetic nitrogen fertilizer will consume a large amount of non-renewable petrochemical raw materials, such as natural gas, oil and coal. With the continuous reduction of limited reserves of natural energy, the price of oil and natural gas will continue to rise, which will inevitably drive up the price of chemical fertilizers. The chemical fertilizer industry is also an important source of environmental pollution and greenhouse gases. Fertilizers, when applied to fields, can cause soil compaction, reducing the productive potential of the land. The absorption and utilization rate of chemical fert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/41
Inventor 逄镜萍张菁
Owner 黑龙江省华龙生物科技有限公司
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