Complex microbial inoculum 707 and preparation method and application thereof

A compound bacterial agent and microbial technology, applied in the field of biological materials, can solve the problems that chemical fertilizers cannot meet sustainable development, cannot become the pillar of green agriculture, and chemical element residues, etc., to improve fruit quality, improve fertilizer utilization rate, and promote crop growth Effect

Inactive Publication Date: 2010-12-15
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantages of long-term use of chemical fertilizers have followed, and a series of problems such as soil compaction, chemical element residues, a...

Method used

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  • Complex microbial inoculum 707 and preparation method and application thereof
  • Complex microbial inoculum 707 and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Adopt the microbial composite bacterial agent 707 (hereinafter referred to as 707) prepared by the present invention to apply effect test in paddy planting:

[0027] 1. Seed soaking test

[0028] 1. Test materials and methods

[0029] Test variety: Xiushui 110 (japonica rice)

[0030] experiment method:

[0031] Effects of seed treatments on germination rate and germination vigor. Set up 4 treatments: (1) 707 (1:600 ​​times); (2) 707 (1:400 recommended dose); (3) 707 (1:200 times); (4) CK (water treatment).

[0032] Investigation content: After soaking (mixing) the seeds, treat them according to the requirements, then accelerate germination, measure the germination rate, germination potential, root and bud growth status.

[0033] 2. Test results and analysis

[0034] The results of the investigation found that different treatments had no significant effect on the germination rate of rice seeds. No. 2 treatment had the best promotion effect on the elongation of the...

Embodiment 2

[0046] The application effect test in lettuce plantation by adopting the microbial composite bacterial agent 707 (hereinafter referred to as 707) prepared by the present invention:

[0047] 1. Test materials and methods

[0048] Varieties tested: lettuce with sharp leaves.

[0049] experiment method:

[0050] Set up 4 treatments: (1) CK (conventional fertilization); (2) conventional fertilization + 707 (500 times liquid foliage spraying twice); (3) conventional fertilization + 707 (800 times liquid watering once, 500 times (4) 80%+707 of conventional fertilization rate (800 times of liquid spraying once, 500 times of liquid spraying once).

[0051] Investigation content: After soaking (mixing) the seeds, treat them according to the requirements, then accelerate germination, measure the germination rate, germination potential, root and bud growth status.

[0052] 2. Test results and analysis

[0053] (1) Effects of each treatment on the yield of lettuce

[0054] Effects of...

Embodiment 3

[0061] Adopt the microbial composite bacterium agent 707 (hereinafter referred to as 707) prepared by the present invention to apply effect test in potato planting:

[0062] 1. Test materials and methods

[0063] Varieties tested: Shiquan Longshu No. 3, Xigong Dabaihua, Ningyuan K299.

[0064] (1) Seed soaking test

[0065] Test sites: Shanzhuang Village, Shiquan Township and Huagou Village, Xigong Township. The previous stubble was bean stubble, and the soil fertility was medium.

[0066] Three treatments were set up in the experiment: (1) Soaking seeds of 707; (2) Soaking seeds of Handibao; (3) Not soaking seeds (CK).

[0067] (2) Spraying test

[0068] Test location: Hongtu Village, Ningyuan Town, where the previous stubble was soybean stubble, and the soil fertility was medium.

[0069] Three treatments were set up in the test: (1) 707 spraying; (2) 0.2% potassium dihydrogen phosphate spraying; (3) clear water spraying (CK).

[0070] 2. Result analysis

[0071] In th...

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Abstract

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent 707 and a preparation method and application thereof, and belongs to the technical field of biological materials used as plant biological regulators in crop planting. Background technique [0002] The birth of chemical fertilizers brought farmers hope for a good harvest in a certain period of time. However, the disadvantages of long-term use of chemical fertilizers have followed, and a series of problems such as soil compaction, chemical element residues, and environmental pollution have surfaced. Chemical fertilizers can no longer meet the needs of sustainable development, let alone become the pillar of green agriculture. [0003] With the development of science, the role of microorganisms in agriculture has gradually emerged. The use of microbial agents to improve soil, degrade chemical residues, improve the ecological environment, increase crop production, inhibit bacteria and prevent d...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/20A01N63/02A01N63/04A01P21/00C12R1/125C12R1/10C12R1/11C12R1/72C12R1/41C12R1/07C12R1/25
CPCY02A40/10
Inventor 崔国臣崔国亮刘美娜
Owner DALIAN SANKE BIO ENG
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