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Method for mutually promoting growth of Bradyrhizobium japonicum and Chlamydomonas reinhardtii

A technology of soybean rhizobia and rhizobia, applied in the field of biological hydrogen production, can solve the problems of algae biomass accumulation and other problems

Inactive Publication Date: 2014-09-03
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the use of certain bacteria and algae co-cultivation may promote the biomass accumulation of algae

Method used

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  • Method for mutually promoting growth of Bradyrhizobium japonicum and Chlamydomonas reinhardtii
  • Method for mutually promoting growth of Bradyrhizobium japonicum and Chlamydomonas reinhardtii
  • Method for mutually promoting growth of Bradyrhizobium japonicum and Chlamydomonas reinhardtii

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Experimental program
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Embodiment 1

[0011] Co-cultivation in the normal medium of embodiment 1

[0012] The transgenic Chlamydomonas reinhardtii hemHc-lbac was grown to mid-log phase (OD 750 The value is about 2.0), and the rhizobia were cultured in the YEM medium to the late logarithmic phase (OD 600 value is about 1.0), centrifuged at 4000g for 5 minutes, and washed with TAP medium for 3 times to remove the influence of the original TAP medium and YEM medium; according to the volume mixing ratio of algal liquid and bacterial liquid of 20:1, the rhizobia and Chlamydomonas reinhardtii were added to the Erlenmeyer flask for mixed culture. The culture conditions are: temperature 25℃±1℃, light intensity 60μmol·m -2 ·s -1 .

Embodiment 2-7

[0014] Except the following table parameter, other is the same as embodiment 1

[0015]

[0016] The Chlamydomonas strains of Examples 6 and 7 are derived from patent 201210007835

Embodiment 8

[0018] will be cultured in normal TAP medium to saturation phase (OD 750 value is about 3.0), centrifuge at 4000 r / min for 5 min, remove the supernatant, and wash with sulfur-deficient medium for 3 times to completely remove the sulfur element in the original medium [3] , and then Chlamydomonas reinhardtii according to the concentration OD 750 The value is about 2.0 and transferred to a 60ml hydrogen production culture tube. Will be cultured in YEM medium to late logarithmic phase (OD 600 About 1.0) rhizobia, centrifuge at 4000r / min for 5 minutes, remove the supernatant, wash with sulfur-deficient medium for 3 times, and then add it to the above-mentioned culture tube with Chlamydomonas reinhardtii according to the volume ratio of bacteria and algae at 20:1 In the medium, adjust the volume to 45ml with sulfur-deficient medium, and finally seal the nozzle with a rubber stopper. At a temperature of 25°C±1°C, the hydrogen production tube was shaded and incubated in the dark fo...

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Abstract

The invention relates to the technical field of biological hydrogen production and particularly relates to a method for mutually promoting growth of Bradyrhizobium japonicum and Chlamydomonas reinhardtii.. The method comprises the following steps: respectively co-culturing Bradyrhizobium japonicum (hereinafter referred to as rhizobia) and another three strains, namely Chlamydomonas reinhardtii strain cc124 (hereinafter referred to as 124), algal strains cc503 (hereinafter referred to as 503) and transgenic Chlamydomonas reinhardtii hemHc-lbac (hereinafter referred to as hemHc-lbac) in a normal culture medium and a sulfur-deficient culture medium, the results show that the growth of Chlamydomonas reinhardtii and Bradyrhizobium japonicum can be promoted and the Bradyrhizobium japonicum gathers around the Chlamydomonas cell and grows prior to the hydrogen production decrescence, which indicates that a phenomenon of a periodical reciprocal symbiosis exists between Chlamydomonas reinhardtii and rhizobia. The method disclosed by the invention provides an experimental basis and a new idea for further broadening the host range of rhizobia, promoting the biomass accumulation of large-scale cultivation of Chlamydomonas reinhardtii, producing a renewable biological energy source and improving the ecological environment.

Description

technical field [0001] The invention relates to a biohydrogen production technology, in particular to a method for promoting the growth of Brady rhizobium and Chlamydomonas reinhardtii. Background technique [0002] The massive use of fossil fuels such as coal, oil, and natural gas has caused serious environmental problems; on the other hand, the reserves of these energy sources are limited, and the long-term unrestrained use has caused the current energy shortage. Hydrogen is a renewable bioenergy with clean combustion, high combustion calorific value, and various forms of utilization. It is an important part of the future clean energy system. Microalgae photosynthetic hydrogen production technology is a technology that uses microalgae and solar energy to produce hydrogen under special conditions, and is considered to be an important way for the sustainable and clean production of hydrogen in the future. Chlamydomonas reinhardtii ( Chlamydomonas reinhardtii ) has high hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12C12R1/01C12R1/89
Inventor 吴双秀李德志许丽丽王全喜
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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