Preparation process of organochlorine pesticide contaminated soil remediation microbial agent

A technology for pesticide-contaminated soil and repairing bacterial agents, applied in the field of environmental protection, can solve problems such as polluted soil, achieve high degradation ability, increase degradation rate, and have obvious effects of co-metabolism and degradation

Inactive Publication Date: 2020-12-01
森科环保新材料(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no microbial remediation agent specially designed for organochlorine pesticide-contaminated soil, and it is still a research blank

Method used

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  • Preparation process of organochlorine pesticide contaminated soil remediation microbial agent
  • Preparation process of organochlorine pesticide contaminated soil remediation microbial agent
  • Preparation process of organochlorine pesticide contaminated soil remediation microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:

[0033] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 2kg liquid culture medium respectively, and culture them in the dark at 26°C and 100rpm for 18h; Described liquid culture medium comprises the peptone of 0.8%, the yeast powder of 0.05%, the sodium chloride of 0.9%, the agar of 1.5% according to mass kg number;

[0034] Step 2, the treatment of the filler, mix 80kg of wheat bran and 50kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 5 minutes, and then the filler is sterilized by steam for 20 minutes, and then cooled to room temperature ;

[0035] Step 3, add all the expanded microbial culture medium into the fermenter, contro...

Embodiment 2

[0046] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:

[0047] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 4kg liquid medium respectively, and culture them in the dark at 28°C and 130rpm for 24h; The liquid medium includes 0.8%-1.6% peptone, 0.05%-0.1% yeast powder, 1.2% sodium chloride, and 2% agar according to the mass kg;

[0048] Step 2, the treatment of the filler, mix 100kg of wheat bran and 70kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 8 minutes, and then the filler is sterilized by steam for 25 minutes, and then cooled to room temperature ;

[0049] Step 3, add all the expanded microbial culture medium into the fermenter, control the temperature at 28°C, and ferment an...

Embodiment 3

[0060] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:

[0061] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 5kg liquid medium respectively, and culture them in the dark at 30°C and 150rpm for 36h; Described liquid culture medium comprises the peptone of 1.6%, the yeast powder of 0.1%, the sodium chloride of 1.5%, the agar of 2.5% according to mass kg number;

[0062] Step 2, the treatment of the filler, mix 120kg of wheat bran and 80kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 10 minutes, and then the filler is sterilized by steam for 30 minutes, and then cooled to room temperature ;

[0063] Step 3, add all the expanded microbial culture medium into the fermenter, control the t...

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Abstract

The invention relates to the field of environmental protection, in particular to a preparation process of an organochlorine pesticide contaminated soil remediation microbial agent. The composite soilremediation microbial agent is prepared by taking rhizobium which is preferably selected from rhizobium japonicum as a main component, and taking nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, trichoderma and the like as auxiliary materials. The microbial agent has a very good decomposition effect on organochlorine pesticides, has the advantages which are not possessed by a single bacterial colony, and has a more beneficial effect on degradation of the organochlorine pesticides. The composite microbial agent provided by the invention takes crop waste residues wheat bran and corn straw as carrier materials, and also has the effect of fertilizing soil. The composite microbial agent has relatively high degradation capacity on pollutants, and can play a role respectively in the presence of multiple pollutants. According to the invention, a complex and stable composite microbial ecological system capable of playing multiple functions is formed through the symbiotic relationship of multiple microbial agents.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to a preparation process of an organochlorine pesticide-contaminated soil remediation bacterial agent. Background technique [0002] As a large agricultural country with a large population, my country has long been accustomed to using various pesticides to control diseases and insect pests and eradicate weeds in the process of crop planting. As a necessary means of production, pesticides play a pivotal role in ensuring high agricultural yields and harvests. Organochlorine pesticides are persistent organic pollutants that widely exist in environmental media such as air, water, soil, and sediment. At present, the remediation technology of organochlorine pesticide-contaminated soil can be divided into physical and chemical remediation, biological remediation, and composite remediation combining multiple remediation technologies. Restoration medicine is an important material in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14B09C1/10C09K17/14C12R1/41C12R1/065C12R1/885C12R1/07C09K101/00
CPCB09C1/10C09K17/14C09K2101/00C12N1/14C12N1/20
Inventor 不公告发明人
Owner 森科环保新材料(广州)有限公司
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