Preparation process of organochlorine pesticide contaminated soil remediation microbial agent
A technology for pesticide-contaminated soil and repairing bacterial agents, applied in the field of environmental protection, can solve problems such as polluted soil, achieve high degradation ability, increase degradation rate, and have obvious effects of co-metabolism and degradation
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Embodiment 1
[0032] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:
[0033] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 2kg liquid culture medium respectively, and culture them in the dark at 26°C and 100rpm for 18h; Described liquid culture medium comprises the peptone of 0.8%, the yeast powder of 0.05%, the sodium chloride of 0.9%, the agar of 1.5% according to mass kg number;
[0034] Step 2, the treatment of the filler, mix 80kg of wheat bran and 50kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 5 minutes, and then the filler is sterilized by steam for 20 minutes, and then cooled to room temperature ;
[0035] Step 3, add all the expanded microbial culture medium into the fermenter, contro...
Embodiment 2
[0046] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:
[0047] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 4kg liquid medium respectively, and culture them in the dark at 28°C and 130rpm for 24h; The liquid medium includes 0.8%-1.6% peptone, 0.05%-0.1% yeast powder, 1.2% sodium chloride, and 2% agar according to the mass kg;
[0048] Step 2, the treatment of the filler, mix 100kg of wheat bran and 70kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 8 minutes, and then the filler is sterilized by steam for 25 minutes, and then cooled to room temperature ;
[0049] Step 3, add all the expanded microbial culture medium into the fermenter, control the temperature at 28°C, and ferment an...
Embodiment 3
[0060] A preparation process of organochlorine pesticide-contaminated soil remediation bacterial agent, its specific preparation scheme is as follows:
[0061] Step 1, the expansion of microorganisms, inoculate the preferred rhizobia, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, Trichoderma and bacillus into 5kg liquid medium respectively, and culture them in the dark at 30°C and 150rpm for 36h; Described liquid culture medium comprises the peptone of 1.6%, the yeast powder of 0.1%, the sodium chloride of 1.5%, the agar of 2.5% according to mass kg number;
[0062] Step 2, the treatment of the filler, mix 120kg of wheat bran and 80kg of corn stalks evenly and put them into the fermenter. The fermenter is sterilized by steam for 10 minutes, and then the filler is sterilized by steam for 30 minutes, and then cooled to room temperature ;
[0063] Step 3, add all the expanded microbial culture medium into the fermenter, control the t...
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