67results about How to "Toxic effect" patented technology

The invention provides a method for preparing an antibacterial coating on the surface of a titanium implant. A silver-loaded polyelectrolyte multilayer film is prepared on the titanium surface with a layer-layer self-assembly method, so that the titanium surface has good biocompatibility and antibacterial performance. The method is simple and particularly suitable for implants in complex shapes; the surface coating adopts natural polymers with good biocompatibility, so that the coating has bacterium killing and inhibiting properties and causes no toxic effect to cells.

The invention relates to a pollution-free livestock and poultry feed additive using ginkgo leaves as a base stock. The pollution-free livestock and poultry feed additive is prepared from the following materials in parts by weight: 150-200 parts of ginkgo leaves, 100-120 parts of potatoes, 7-10 parts of hawthorns, 5-8 parts of garlic, 4-7 parts of dried gingers, 3-5 parts of Chinese mosla herbs, 2-3 parts of poria cocos, 5-7 parts of sweet wormwood herbs, 2-3 parts of roots of chinese pulsatilla, 7-10 parts of purslane, 3-6 parts of stone-like omphalia, 10-15 parts of pine needles and 10-13 parts of medical stones. According to the pollution-free livestock and poultry feed additive using ginkgo leaves as the base stock disclosed by the invention, a special process is adopted to treat the ginkgo leaves, and a plurality of traditional Chinese medicine components are combined, so that purposes of improving animal immunity and promoting animals to grow can be achieved very well; moreover, the feed additive disclosed by the invention completely adopts natural components, is not extracted and treated by any harmful chemical reagent, and satisfies green, safe and pollution-free requirements of modern breeding industry.

The invention discloses a calcium-phosphorus-silicon biological ceramic coating and a preparation method and application thereof. After magnesium and alloy of magnesium are put in a calcium-phosphorus electrolyte for powered-on electrolysis for a set time, a sample containing a calcium-phosphorus coating is put in a silicon salt electrolyte for powered-on electrolysis, and the target biological ceramic coating is obtained; and the calcium-phosphorus electrolyte is prepared by adding calcium salt and (NaPO3)6 into a basic electrolyte, and the silicon salt electrolyte is prepared by adding silicate into the basic electrolyte. A compact layer is connected with a basal body, the compact layer and the basal body are tightly combined, and the mechanical property of the basal body can be improved; in addition, pores barely exist in the compact layer, body fluid can be prevented from flowing to the surface of the basal body to make contact with the basal body, metal ions generated after the basal body is corroded also can be prevented from being diffused to the human body, toxicity is reduced, and biocompatibility is effectively improved; and a surface layer is a porous loose layer, combination of an implant and a bone can be effectively improved in the presence of micro-pores, growth of adherence bone tissue of bone cells can be effectively improved, and the biological activity of the coating can be improved.

The invention discloses a ferro-manganese double metal oxide modified bio-carbon photo-Fenton composite material and a preparation method thereof. The composite material comprises bio-carbon and ferro-manganese double metal oxide attached to the bio-carbon, wherein the ferro-manganese double metal oxide is a mixture of iron oxide and manganese oxide; the iron oxide comprises Fe3O4 and Fe2O3; and the manganese oxide comprises Mn3O4 and Mn2O3. The preparation method comprises the steps of preparing a ferro-manganese double metal oxide modified bio-carbon precursor and calcining the ferro-manganese double metal oxide modified bio-carbon precursor. The composite material takes the bio-carbon as a main body material and the ferro-manganese double metal oxide as a modification material, combinesthe advantages of the bio-carbon and the ferro-manganese double metal oxide, has the advantages of excellent catalytic performance, high stability, high recoverability, low cost, environmental friendliness and the like, and has a good degradation effect on polycyclic aromatic hydrocarbon in water. The preparation method has the advantages of mild reaction condition, simple operation flow, environmental friendliness and the like, and can be applied to large-scale production and preparation.

A beverage for clearing away the lung heat and making throat comfortable is prepared from 11 Chinese-medicinal materials including liquorice root, dried rehmannia root, bamboo leaf, yam, etc.

The invention relates to an ecological management system for excrement liquid of livestock and poultry. The ecological management system comprises a source-separation water-saving house, a pollution treatment factory, a fodder factory and an auxiliary planting land. The pollution treatment factory comprises a high-temperature solid aerobiotic-fermentation system, a middle-temperature liquid anaerobic-fermentation system, a heating and heat balance system and a detection control system. A regulation pool is connected with a feed inlet of the middle-temperature liquid anaerobic-fermentation system through a delivery pump, methane generated by the middle-temperature liquid anaerobic-fermentation system is delivered to the heating and heat balance system, and the heating and heat balance system is connected a heating coil of the middle-temperature liquid anaerobic-fermentation system through a pipeline. The ecological management system has the advantages that finished products, namely liquid organic and inorganic compound fertilizer and liquid microbial fertilizer, of the pollution treatment factory are used for irrigation and drop irrigation of plants planted on the auxiliary plantingland, and pastures are taken as fodder raw materials for the fodder factory, so that cultivation cost is reduced, nutrition level of fodder is improved, and harmonious development of the breeding industry and the biological environment is promoted.

The invention relates to a pollution-free aquatic feed additive using ginkgo leaves as a base stock. The pollution-free aquatic feed additive is prepared from the following materials in parts by weight: 150-200 parts of ginkgo leaves, 100-120 parts of potatoes, 30-50 parts of lotus leaves, 15-20 parts of common andrographis herbs, 8-12 parts of Chinese mosla herbs, 5-7 parts of Chinese rhubarb, 7-12 parts of mangnolia officinalis, 3-5 parts of negundo chastetree fruits, 50-80 parts of chlorella powder, 50-80 parts of egg powder, 5-10 parts of medical stones and 20-30 parts of corn starch. The feed additive disclosed by the invention completely adopts natural components, is not extracted and treated by any harmful chemical reagent and pollution-free to an aquaculture environment, and completely satisfies green, safe and pollution-free requirements of modern aquaculture industry.

The present invention relates to test technology and is especially high-sensitivity detection of singlet state oxygen and superionic oxygen. Into 1-100 um / L concentration chemical luminous probe solution, albumin in 1-100 um / L concentration is added. The said method has the features of small amount of required specimen, simple operation, no need of expensive instrument and chemical, high sensitivity and low concentration detection capacity.

The invention discloses a strain of Ensifer adhaerens dt8 and application of the strain in the degradation of paclobutrazol. The strain of the Ensifer adhaerens dt8 is preserved in Guangdong MicrobialCulture Collection Center (GDMCC) on April 3, 2018 and the preservation number is GDMCC No: 60351. The strain of the Ensifer adhaerens dt8 provided by the invention is capable of secreting extracellular substances to degrade the paclobutrazol in a process of growth and metabolism, can be applied to preparation of fungicides for degrading the paclobutrazol in soil, can be applied to degradation ofthe paclobutrazol in cultivated soil, relieves the stress effect of inhibiting apical growth of the paclobutrazol on crops or other plants, reduces the possibility that the crops carry the paclobutrazol into a food chain, and has a relatively great application prospect.

The invention discloses a method for preparing a calcium silicon phosphor biological ceramic coating through two steps and application. Magnesium and magnesium alloy base bodies are put into silicate electrolyte to be subjected to electrification electrolysis for a set time; then, a test specimen containing a silicon coating is put into calcium phosphor electrolyte to be subjected to electrification electrolysis; a target biological ceramic coating is obtained; the silicate electrolyte is prepared through adding silicate into basic electrolyte; the calcium phosphor electrolyte is prepared through adding calcium salts and (NaPO3)6 in basic electrolyte. The thickness of the calcium silicon phosphor coating prepared by the method is 30mum to 120mum; the coating comprises ingredients of MgO, MgF2, MgSO3, Ca2P4O7 and the like. The appearance of the surface of the ceramic coating can be changed by controlling the base body composition, the electrolyte ingredients and the electric parameters; the surface micropore dimension is controlled, so that the bone cell and bone tissue growth is facilitated in living body embedding experiments.

The present invention discloses a targeting ligand-drug conjugate having a cell endocytosis mediating function. The targeting ligand-drug conjugate comprises a drug molecule and a linker coupled to the drug molecule, and is characterized by further comprising a molecule having a mediating effect and a ligand specifically bound to a cell surface receptor, wherein the ligand is a polypeptide or small molecule ligand, and the molecule can be coupled to the ligand, can be coupled to the drug molecule or linker to form the drug conjugate, or is respectively and separately coupled to one of the drug molecule and the linker to form the drug conjugate. According to the present invention, with the combination of the targeting and the endocytosis (transmembrane) structure, the any ligand targeting polypeptide can be adopted as the leader so as to broaden the targeting range of the drug; and with the conjugate, the stability of the drug during the in vivo circulation is ensured, and the drug toxicity is reduced.

The invention discloses a drug conjugate with multiple targeting ligands having a cell endocytosis mediating function. The drug conjugate consists of drug molecules and connexons coupled with the drug molecules; the drug conjugate at least comprises two or more ligands which can be specifically bound to cell surface receptors; the ligands at least include one first ligand which has the cell endocytosis mediating function; and at least one of the ligands and the first ligand are coupled and then are selectively coupled with the drug molecules or the connexons so as to form the drug couplet. The drug conjugate provided by the invention has the beneficial effects that by combining targeting and endocytosis structures, polypeptide, which is targeted by any ligands, is taken as a guiding part, so that the targeting scope of the like medicines is widened. The affinity and the targeting performance of the drug conjugate on diseased cells can be enhanced by virtue of the multiple targeting ligands, so that efficient toxin drugs such as MMAE can be carried, efficacy can be enhanced, a therapeutic window can be widened and side effects of the medicines can be avoided.

The invention relates to a heavy metal soil remediation agent and method based on magnesium oxide-montmorillonite composite material synergistic composting, belonging to the technical field of heavy metal soil remediation. The heavy metal soil remediation agent comprises a magnesium oxide-montmorillonite composite material and compost. The montmorillonite has a layered structure. The magnesium oxide-montmorillonite composite material is a material in which magnesium oxide is loaded on the surface and interlayer of the montmorillonite. The remediation agent and the remediation method can enableheavy metals to form stable chemical forms and complete remediation of heavy metal soil. The method has the advantages of low cost, simplicity, high efficiency, environmental friendliness and the like, and has good remediation effect on soil polluted by single cadmium pollution and composite heavy metals.

The invention relates to an autologous adipose cell cryopreservation solution and a cryopreservation method of substances such as adipose tissue-derived stromal cells. Every 100 mL of the autologous adipose cell cryopreservation solution comprises the following content components: 20 to 38 mL of mesenchymal stem cell culture supernatant, 3 to 5 g of polyvinylpyrrolidone, 3 to 5 mL of glycerinum, 1.5 to 2 g of hydroxyapatite nanoparticles, 1.5 to 2 g of trehalose, 0.5 to 1 g of polyvinyl alcohol, 0.01 to 0.1 g of human serum albumin, 0.05 to 0.3 g of adenosine triphosphate, 0.5 to 1.5 mg of all-trans retinoic acid, 0.3 to 0.8 g of vitamin C, 0.2 to 0.6 g of vitamin E and 0.2 to 0.6 g of lipoic acid. Compared with the prior art, ice crystal formation can be effectively reduced, and cell damage caused by uneven temperature conduction and sharp ice crystal formation is prevented.

The invention discloses a method for repairing soil polluted by heavy metals by using a magnesium silicate-hydrothermal carbon composite material. The method comprises the step that the magnesium silicate-hydrothermal carbon composite material is fully mixed with the soil polluted by the heavy metals, and therefore the magnesium silicate-hydrothermal carbon composite material reacts with the heavy metals in the soil, the heavy metals can form stable chemical forms, and the soil polluted by the heavy metals is repaired. The magnesium silicate-hydrothermal carbon composite material comprises blocky porous magnesium silicate and spherical porous hydrothermal carbon, and the hydrothermal carbon is loaded on the surface and in the pores of the magnesium silicate. The method has the advantages of being low in cost, simple, high-efficiency and environmental-friendly, and has good repairing effects on the soil polluted by single cadmium and the soil polluted by the composite heavy metals.

The invention provides an artery clamp capable of tightening step by step. The artery clamp comprises a pressing piece, a reserved coil with multiple slip knots and a stray line marked with indicating graduations, the pressing piece comprises a first pressing piece body and a second pressing piece body, a slot is arranged on the first pressing piece body, a fixing through hole is arranged on the second pressing piece body, the reserved coil is fixed on the second pressing piece body, a plug pin matched with the slot is arranged at one end of the stray line, and the other end of the stray line penetrates out of the reserved coil via the fixing through hole. The invention further provides a progressive carotid artery ligation system which comprises the artery clamp and a knot guide. Using the progressive carotid artery ligation system for parent artery occlusion needs no reopening a wound, secondary operation and anesthetization are avoided, risks of operative complications can be reduced, operative incision healing time is shortened, operative and hospitalization expenses are saved, and pain of a patient is relieved; the pressing piece of the artery clamp is made of biocompatible materials and is non-toxic to human tissues; and the artery clamp is simple in integral structure, low in cost and convenient to use.

The invention discloses a novel non-polluting water purification agent formula and a preparation method thereof. A water purification agent is prepared from the raw materials such as water-soluble silicon, Chinese herbal medicine extracts, magnesium silicate, activated carbon, sulfate, sodium chlorate, ammonium bicarbonate, chlorine dioxide, polymerized aluminum chloride, chitosan and polymerizedaluminum-iron phosphate through a series of process steps. When the water purification agent is applied into water, shock waves can be generated, thus impurities in the water are effectively dissolved, meanwhile, the concentration of dissolved oxygen is increased, sufficient oxygen is provided, and thus the growth and development of fishes and shrimps are facilitated; no toxic substance exists, sterilization can be effectively conducted, the toxic effect on a human body is also avoided, and safety is high; and after the water purification agent is applied, excessive multiplication of algae inwater bodies can be effectively inhibited, the eutrophication state of the culture water bodies is improved, the significant effect is achieved on repairing the water environment of a culture system,a good water area ecological environment can be created for high yield and efficient culture of the shrimps, the growth of the fishes and the shrimps is promoted, and the quality of the fishes and theshrimps is improved.

The invention belongs to the field of PDA (potato dextrose agar) culture medium preparing methods and discloses a high-transparency PDA culture medium preparing method.The method includes steps: A, potato dicing; B, stewing, to be more specific, stewing over strong fire and then slow fire to make potato dices thoroughly cooked but not mushy; C, filtering; D, material adding, to be more specific, adding 20g of glucose, 0.8-1.4g of monopotassium phosphate and 1.2-1.7g of magnesium sulfate into filtrate obtained at the step C, well mixing, and adding water to a constant volume of 1000mL; E, pH value regulation, to be more specific, using a hydrochloric acid and a sodium hydroxide solution for regulating the pH value to 6.5-7 to obtain a liquid culture medium, distributing 19-23g of high-purity agar powder into a plurality of mixing containers, then distributing the liquid culture medium into the mixing containers, and well mixing; F, transferring to flat-plate petri dishes; G, dehumidifying, namely putting the petri dishes over flame of an alcohol lamp to remove moistures; H, sealing and sterile testing.The high-transparency PDA culture medium preparing method has the advantages that raw materials can be proportionally mixed precisely and quickly, and system errors and operation errors are avoided; moistures in the petri dishes can be well controlled to avoid breeding of contaminating microorganisms.

The invention belongs to the technical field of edible fungus culture and particularly relates to a ganoderma lucidum culture medium added with jasmine flower residues and a preparation method thereof. The ganoderma lucidum culture medium added with the jasmine flower residues is prepared from, by weight, 10-20 parts of jasmine flower residues, 30-40 parts of oilseed rape straws, 10-20 parts of bagasse, 10-20 parts of corncobs, 30-40 parts of ganoderma lucidum residues, 10-30 parts of ginger straws, 10-20 parts of bran, 20-30 parts of soybean meal and 1-3 parts of light calcium carbonate. The culture medium does not contain sawdust, and produced ganoderma lucidum is large in yield and rich in nutrition.

The invention discloses an antibacterial composite biological material containing agarose acetate and a preparation method thereof. An antibacterial drug is added to a biologically-inertial agarose acetate electrospun fibrous membrane as a base; hydrogen bonds on the surface of the surface drug of composite fiber can be utilized so that antibacterial particles are firmly supported to the surface of electrospun fiber; therefore, the antibacterial composite biological material containing the agarose acetate is provided. The antibacterial composite biological material can prevent nutrient supplyof healing field tissues from being affected, and normal healing mechanism of the tissues is maintained; during release of the antibacterial drug, the material can degrade and disappear; degradation products never cause toxic and harmful effects to the tissues and the body, and the material can truly promote healing of antibacterial tissues. The antibacterial composite biological material has goodmechanical properties and biological compatibility and has degrading time that matches with healing time of infected tissues; the antibacterial composite biological material is suitable for antibacterial materials, regenerative medical research and clinical practice.

The invention belongs to the technical field of edible mushroom cultivation and particularly relates to a lentinula edodes culture medium including, by weight, 40-50 parts of paddy rice straw, 20-30 parts of lucid ganoderma residue, 20-30 parts of beer residue, 30-40 parts of cane sugar, 1-3 parts of vitamin B, 1-3 parts of vitamin D, 10-20 parts of peanut shells, 20-30 parts of chicken manure, 5-10 parts of plant ash and 1-3 parts of urea. The culture medium is free of wood chips. Cultured lentinula edodes is high in yield and has abundant nutrients.

The invention relates to the field of environmental protection, in particular to a preparation process of an organochlorine pesticide contaminated soil remediation microbial agent. The composite soilremediation microbial agent is prepared by taking rhizobium which is preferably selected from rhizobium japonicum as a main component, and taking nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, trichoderma and the like as auxiliary materials. The microbial agent has a very good decomposition effect on organochlorine pesticides, has the advantages which are not possessed by a single bacterial colony, and has a more beneficial effect on degradation of the organochlorine pesticides. The composite microbial agent provided by the invention takes crop waste residues wheat bran and corn straw as carrier materials, and also has the effect of fertilizing soil. The composite microbial agent has relatively high degradation capacity on pollutants, and can play a role respectively in the presence of multiple pollutants. According to the invention, a complex and stable composite microbial ecological system capable of playing multiple functions is formed through the symbiotic relationship of multiple microbial agents.

The invention discloses a serum-free cell cryopreservation solution, a preparation method and a use method thereof, and relates to the technical field of biological medicines. The serum-free cell cryopreservation solution is prepared from the following components: 4 to 6 g / 100mL of polyethylene glycol, 0.5 to 2.5 g / 100mL of trehalose, 2 to 3 g / 100mL of glucose, 0.5 to 1.5 mg / 100mL of all-trans retinoic acid and 0.5 mg / 100ml of a small amount of ascorbic acid, a serum-free basal culture medium is used as a solvent, and the final pH value is 6.8 to 7.4. The using method comprises the steps of digestion, cryopreservation and resuscitation. The cell cryopreservation solution is clear in components, does not contain exogenous serum or organic solvents, avoids exogenous protein pollution, and can be directly cryopreserved at -80 DEG C without programmed cooling; the activity of the stem cells can be effectively protected, and the activity of the freeze-thawed cells is maintained and prevented from being lost. According to the cryopreservation method, the risk that the cells absorb water and burst is reduced during recovery, and the cell activity is guaranteed.

The invention discloses a method for storing a micro-algae bait. The method comprises the following steps: uniformly mixing a PH buffering solution, a cell protective agent and concentrated organic acids, preservatives and antibiotic solutions with the high-cell-concentration micro-algae bait which is obtained by performing fermentation according to certain volume ratios to obtain a mixture; subsequently, putting the mixture under a light-proof condition that a temperature is 0 DEG C to 10 DEG C and storing the mixture. In the method, the added materials cannot produce toxic and harmful effects on micro-algae cells, on the contrary, an acid-alkali system of the micro-algae bait also can be maintained, so that pollution of mixed bacterium during preservation is avoided, the biological activity of the micro-algae bait is maintained to the maximum extent, the consumption of the antibiotics and the preservatives brought into a water body is relatively low, and pollution to the water body is avoided.

The invention discloses an ointment with effects of diminishing inflammation and relieving pain and a preparation method thereof. The preparation method comprises the following steps: 1, mixing and grinding salvia miltiorrhiza, ligusticum wallichii, panax notoginseng, coptis chinensis and astragalus, soaking, performing reflux heating, and filtering to obtain traditional Chinese medicine extract I; 2, decocting, filtering and concentrating wormwood and lonicera japonica to obtain water extract II; 3, grinding and juicing leek, and filtering to prepare ester extract III; 4, mixing and concentrating the traditional Chinese medicine extract I, the water extract II and the ester extract III to prepare a concentrated solution; 5, grinding Chinese chestnut and lotus seed into powder so as to obtain mixed powder IV; 6, mixing honey, mixed powder IV and the concentrated solution to prepare emulsion V; 7, mixing vaseline, aloe vera gel and the emulsion V to prepare the ointment with effects ofdiminishing inflammation and relieving pain; 8, performing high pressure homogenization, and packaging with aluminum sheet, thereby obtaining the finished product. The ointment with effects of diminishing inflammation and relieving pain prepared by the preparation method disclosed by the invention is specially prepared for ventilation, has soothing and recovering effects on pain and discomfort caused to each part of the human body, can achieve the effects of plaster and oral painkillers, and can also overcome defects of the plaster and oral painkillers.

The invention discloses a deposition type microbial fuel cell for oil sludge treatment and synchronous reduction of soil hexavalent chromium, which comprises a shell, a cathode, an anode, a proton exchange membrane, a conductor and a resistor, wherein the cathode is a graphite plate and is placed on the upper part of soil contaminated with hexavalent chromium; the anode is an open cell foam carbonmaterial and is placed in the oil sludge at the lower part of the oil sludge. A proton exchange membrane is arranged between that cathode and the anode; the cathode and the anode are connected with resistors or electrical equipment through wires. The device does not need additional electric energy input, reduces hexavalent chromium in contaminated soil while degrading pollutants such as petroleumhydrocarbons in oil sludge, and microorganisms in anode can generate electric energy by utilizing organic substances in oil sludge. The invention is an economical and effective technology for degrading petroleum hydrocarbons in oil sludge, simultaneously reducing hexavalent chromium in soil and recovering electric energy.

The invention provides an apidaecin expression vector as well as a construction method and application thereof. The construction method comprises the following steps: 1) synthesizing an expression operon gene containing encoding NTEV protease; 2) synthesizing a fusion protein expression operon gene containing encoding xylanase and apidaecin; 3) conducting double-enzyme digestion on the expression operon genes obtained in the steps 1) and 2) in a separated mode, and constructing into a vector pBluescript II SK(+) so as to obtain a recombinant plasmid; 4) extracting a genome DNA of bacillus subtilis 168, amplifying a promoter p43, cloning by virtue of a T vector so as to obtain a plasmid containing a promoter gene, and constructing into a plasmid pGJ103 so as to obtain a plasmid pGJ284; conducting reverse PCR (polymerase chain reaction) so as to obtain an efficient promoter sequence gene containing partial gene sequences of the p43 promoter, and constructing into the plasmid pGJ284 so as to obtain a plasmid pGJ288; and 5) conducting double-enzyme digestion on the recombinant plasmid and plasmid pGJ288 in a separated mode, recovering enzyme digestion products and linking so as to obtain an apidaecin expression vector. The expression vector is high in expression amount; and the apidaecin extraction method is simple and convenient, and is suitable for industrial large-scale preparation.

The invention discloses a phosphorus-nitrate-type controlled-release bulk blending fertilizer. The fertilizer is prepared from the following raw materials in parts by weight: 20-60 parts of a phosphorus nitrate fertilizer, 10-30 parts of coated urea, and 10-70 parts of a common fertilizer. The total nutrient of the phosphorus nitrate fertilizer is 35%-40%, and the nitrogen content of the coated urea is 33%-46%. The common fertilizer is prepared from urea, monoammonium phosphate, diammonium phosphate, potassium chloride, potassium sulphate, ammonium chloride, and ammonium sulfate. The related phosphorus nitrate fertilizer adopts a technology of decomposing phosphorite by nitric acid, and does not contain biuret. The fertilizer is prepared from two forms of nitrogen sources of nitrate nitrogen and ammonium nitrogen, and two types of phosphorus sources of water-soluble phosphorus and citric acid soluble phosphorus. The fertilizer is rich in medium trace elements and nitrate rare earth activated by nitric acid, and 5 levels of the coated urea released by the coated urea. The fertilizer is capable of completely satisfying soil testing and formulated fertilization promoted in China, anda formula proportion can be adjusted according to a soil testing result. Quick-acting, intermediate-acting and long-acting nutrients can be perfectly combined. The production and income are increasedin the case of reducing a dosage of the fertilizer. A purpose of keeping soil healthy is realized.

The invention belongs to the field of technologies for cultivating edible fungi, and particularly relates to a ganoderma culture substrate and a method for preparing the same. The ganoderma culture substrate comprises, by weight, 30-40 parts of rape straw, 10-20 parts of bagasse, 10-20 parts of corncobs, 30-40 parts of ganoderma residues, 10-30 parts of rhizoma zingiberis recens straw, 10-20 parts of bran, 20-30 parts of bean pulp and 1-3 parts of light calcium carbonate. The ganoderma culture substrate and the method have the advantages that the ganoderma culture substrate does not contain saw dust, and ganoderma produced by the aid of the ganoderma culture substrate is high in yield and contains abundant nutrition.