Method for storing micro-algae bait

Abstract

The invention discloses a method for storing a micro-algae bait. The method comprises the following steps: uniformly mixing a PH buffering solution, a cell protective agent and concentrated organic acids, preservatives and antibiotic solutions with the high-cell-concentration micro-algae bait which is obtained by performing fermentation according to certain volume ratios to obtain a mixture; subsequently, putting the mixture under a light-proof condition that a temperature is 0 DEG C to 10 DEG C and storing the mixture. In the method, the added materials cannot produce toxic and harmful effects on micro-algae cells, on the contrary, an acid-alkali system of the micro-algae bait also can be maintained, so that pollution of mixed bacterium during preservation is avoided, the biological activity of the micro-algae bait is maintained to the maximum extent, the consumption of the antibiotics and the preservatives brought into a water body is relatively low, and pollution to the water body is avoided.

Description

technical field [0001] The invention belongs to the technical field of aquatic living organism bait preservation, and in particular relates to a method for preserving microalgae bait. Background technique [0002] Microalgae are rich in protein, various essential amino acids, polyunsaturated fatty acids, and bioactive substances such as vitamins, minerals, and sterols. Because of these characteristics, microalgae cells are commonly used as living bait in aquaculture, and microalgae The bait can also play a role in purifying water quality. However, when microalgae are used as bait, the number of living microalgae and feeding time must be closely coordinated with the seedling cultivation, and there must be no mistakes, otherwise it will easily cause a disconnect between the supply and demand of bait. At present, the main problem after large-scale cultivation of microalgae is how to ensure the number and activity of living microalgae cells during high temperature, long-distanc...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the bottleneck problems such as unstable properties, unbalanced nutritional components, and untimely supply of microalgae biological bait due to the ubiquitous preservation and transportation process, and reduce the adverse effects of these unfavorable factors on aquatic seedling production, the current preservation of microalgae bait The low-temperature drying technology is mainly used for preservation. This technology mainly has the following problems: (1) The activity of microalgae cells is completely lost, which reduces the palatability, and at the same time affects the dispersion and shape retention of microalgae in water.

(2) Cryopreservation requires the addition of high doses of cell antifreeze, which may pollute the water body when feeding

(3) The operation process is cumbersome, the equipment requirements are high and the storage cost is too high

As for the room temperature preservation method, there are problems such as strong algae metabolism, easy corruption, and not suitable for long-term storage.

Moreover, the storage period of the current cryopreservation technology is generally only 1-2 months, and the cell activity may decrease or even die in the later stage of storage, which seriously affects the use cycle and transportation radius of microalgae bait

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