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Strain of Ensifer adhaerens dt8 and application of strain in degradation of paclobutrazol

A technology of sticking sword bacteria and paclobutrazol, applied in the direction of bacteria, restoration of contaminated soil, and methods based on microorganisms, to achieve the effect of reducing the possibility, relieving the stress effect, and having a large application prospect

Active Publication Date: 2018-11-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on the genus Adherens that can degrade paclobutrazol

Method used

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  • Strain of Ensifer adhaerens dt8 and application of strain in degradation of paclobutrazol
  • Strain of Ensifer adhaerens dt8 and application of strain in degradation of paclobutrazol
  • Strain of Ensifer adhaerens dt8 and application of strain in degradation of paclobutrazol

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Isolation, purification and screening of bacterial strains

[0034] The inventor isolated and purified the microorganisms resistant to the pesticide paclobutrazol from the banana garden soil in Fushan Town, Chengmai County, Hainan Province. The specific separation and purification steps were as follows: the paclobutrazol inorganic salt medium was: 1g of sodium chloride, 0.2g of magnesium chloride, ammonium chloride 0.5g, calcium chloride 0.02g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.5g, distilled water 1000mL, pH natural (6.5), solid plus agar powder 18g / L, sterilized at 121°C for 20 minutes, after sterilization Add 15% paclobutrazol powder 1g.

[0035] The soil sample collected from the banana orchard in Fushan Town, Chengmai County, Hainan Province was crushed with a sterilized calculus, 10g of the soil sample was weighed and suspended in 90mL of phosphate buffered solution (PBS) for 10 minutes, and 100µL of the bacterial solut...

Embodiment 2

[0036]Example 2 Physiological and biochemical characteristics and taxonomic status of bacterial strains

[0037] Identification and preservation of bacterial strains: the dt8 bacterial strain isolated in Example 1 has the following morphology and physiological and biochemical characteristics:

[0038] a. Morphological characteristics of the bacteria: the strain is short rod-shaped.

[0039] b. Morphological characteristics of the colony: the growth rate is fast on the LB agar plate, the colony is round, milky white, raised in the middle, moist and neat at the edge (30°C, grow for 24 hours), and the diameter of the colony is 1.8-2.0 cm. The optimum growth temperature is 30 °C and the optimum growth pH value is 7.

[0040] c. Physiological and biochemical characteristics: facultative aerobic, able to utilize L-glutamic acid, glucose, lactose, maltose, arabinose, arabinitol xylose, xylitol, inositol, tartrate, cellobiose, hippuric acid , Urea, Galactose, Mannose, Rhamnose, Xylo...

Embodiment 3

[0045] Paclobutrazol inorganic salt medium: sodium chloride 1g, magnesium chloride 0.2g, ammonium chloride 0.5g, calcium chloride 0.02g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.5g, distilled water to 1L, pH natural ( 6.5), agar powder 18g, sterilized at 121°C for 20 minutes, after sterilization, add 1g of 15% paclobutrazol powder.

[0046] The isolated and purified sword bacteria ( Ensifer adhaerens ) After activation of the dt8 strain, the bacteria suspension was made, OD 600 =0.2; diluted 10 -2 Afterwards, spread on the sterilized paclobutrazol inorganic salt medium, and cultivate at 37°C for 24-36 hours.

[0047] The result is as image 3 As shown, after the strain dt8 was diluted and spread on the solid medium of paclobutrazol inorganic salt and cultured for 24 hours, the strain could use paclobutrazol as a carbon source for growth.

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Abstract

The invention discloses a strain of Ensifer adhaerens dt8 and application of the strain in the degradation of paclobutrazol. The strain of the Ensifer adhaerens dt8 is preserved in Guangdong MicrobialCulture Collection Center (GDMCC) on April 3, 2018 and the preservation number is GDMCC No: 60351. The strain of the Ensifer adhaerens dt8 provided by the invention is capable of secreting extracellular substances to degrade the paclobutrazol in a process of growth and metabolism, can be applied to preparation of fungicides for degrading the paclobutrazol in soil, can be applied to degradation ofthe paclobutrazol in cultivated soil, relieves the stress effect of inhibiting apical growth of the paclobutrazol on crops or other plants, reduces the possibility that the crops carry the paclobutrazol into a food chain, and has a relatively great application prospect.

Description

technical field [0001] The invention relates to the field of microbial technology and biodegradation treatment of crop medicaments, more specifically, to a strain of sword fungus Ensifer adhaerens dt8 strain and its application in degrading paclobutrazol. Background technique [0002] Paclobutrazol, molecular formula C 15 h 20 N 3 OCl, chemical name (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol, Code-named PP333, a synthetic organic compound with high efficiency and low toxicity plant growth retardant and broad-spectrum fungicide. Paclobutrazol can control vegetative growth, delay stem elongation, and transport more photosynthetic products to reproductive organs, thereby inhibiting plant growth, promoting flower bud differentiation, improving plant lodging and stress resistance, inhibiting bacteria and pests, and making plants Anti-aging and production boosting. However, studies have shown that when soil-applied paclobutrazol is applied to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 谭志远谭习羽谭泽文彭桂香
Owner SOUTH CHINA AGRI UNIV
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