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Autologous adipose cell cryopreservation solution and cryopreservation method of adipose tissue-derived stromal cells and other substances

An adipocyte and stromal cell technology, applied in the field of cell biology, can solve the problems of unsatisfactory effect of cryopreservation of adipose-derived mesenchymal stem cells, low cell recovery rate and cell viability rate, susceptibility to uneven heating, etc., to prevent temperature conduction. Inhomogeneous, does not affect cell growth characteristics, and improves the effect of cryopreservation survival rate

Pending Publication Date: 2020-10-16
乔爱军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned formulas are not effective in freezing and storing adipose-derived mesenchymal stem cells, and the recovery rate and cell viability of cells after recovery are low.
Moreover, the general cell recovery process is prone to uneven heating, secondary crystallization, and greater damage to cells.

Method used

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  • Autologous adipose cell cryopreservation solution and cryopreservation method of adipose tissue-derived stromal cells and other substances
  • Autologous adipose cell cryopreservation solution and cryopreservation method of adipose tissue-derived stromal cells and other substances
  • Autologous adipose cell cryopreservation solution and cryopreservation method of adipose tissue-derived stromal cells and other substances

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Experimental program
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Effect test

Embodiment 1

[0025] The invention provides an autologous adipocyte cryopreservation solution, which comprises the following components: 30mL / 100mL mesenchymal stem cell culture supernatant, 4g / 100mL polyvinylpyrrolidone, 4mL / 100mL glycerin, 1.8g / 100mL hydroxyphosphorus Greystone nanoparticles, 1.8g / 100mL trehalose, 0.6g / 100mL polyvinyl alcohol, 0.06g / 100mL human serum albumin, 0.15g / 100mL adenosine triphosphate, 1mg / 100mL all-trans retinoic acid, 0.5 g / 100mL vitamin C, 0.4g / 100mL vitamin E, 0.4g / 100mL lipoic acid.

Embodiment 2

[0027] The invention provides an autologous adipocyte cryopreservation solution, which comprises the following components: 20mL / 100mL mesenchymal stem cell culture supernatant, 5g / 100mL polyvinylpyrrolidone, 3mL / 100mL glycerin, 2g / 100mL hydroxyapatite Stone nanoparticles, 1.5g / 100mL trehalose, 1g / 100mL polyvinyl alcohol, 0.01g / 100mL human serum albumin, 0.3g / 100mL adenosine triphosphate, 0.5mg / 100mL all-trans retinoic acid, 0.8g / 100mL vitamin C, 0.2g / 100mL vitamin E, 0.6g / 100mL lipoic acid.

Embodiment 3

[0029] The invention provides an autologous adipocyte cryopreservation solution, which comprises the following components: 38mL / 100mL mesenchymal stem cell culture supernatant, 3g / 100mL polyvinylpyrrolidone, 5mL / 100mL glycerin, 1.5g / 100mL hydroxyphosphorus Greystone nanoparticles, 2g / 100mL trehalose, 0.5g / 100mL polyvinyl alcohol, 0.1g / 100mL human serum albumin, 0.05g / 100mL adenosine triphosphate, 1.5mg / 100mL all-trans retinoic acid, 0.3 g / 100mL vitamin C, 0.6g / 100mL vitamin E, 0.2g / 100mL lipoic acid, 0.15-0.4g / 100mL lubricant, the lubricant is any one of PEG-4000 or PEG-6000.

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Abstract

The invention relates to an autologous adipose cell cryopreservation solution and a cryopreservation method of substances such as adipose tissue-derived stromal cells. Every 100 mL of the autologous adipose cell cryopreservation solution comprises the following content components: 20 to 38 mL of mesenchymal stem cell culture supernatant, 3 to 5 g of polyvinylpyrrolidone, 3 to 5 mL of glycerinum, 1.5 to 2 g of hydroxyapatite nanoparticles, 1.5 to 2 g of trehalose, 0.5 to 1 g of polyvinyl alcohol, 0.01 to 0.1 g of human serum albumin, 0.05 to 0.3 g of adenosine triphosphate, 0.5 to 1.5 mg of all-trans retinoic acid, 0.3 to 0.8 g of vitamin C, 0.2 to 0.6 g of vitamin E and 0.2 to 0.6 g of lipoic acid. Compared with the prior art, ice crystal formation can be effectively reduced, and cell damage caused by uneven temperature conduction and sharp ice crystal formation is prevented.

Description

[technical field] [0001] The invention relates to the technical field of cell biology, in particular to a cryopreservation method for autologous adipocyte cryopreservation fluid, adipose mesenchymal stem cells and other substances. [Background technique] [0002] Cryoprotectant refers to a substance that can protect cells from frostbite in an extremely low temperature environment, also known as cell cryopreservation solution. Adding cryoprotectants to cell suspensions can protect cells from solution damage and ice crystal damage. The cryoprotectant combines with the water molecules in the solution to cause a hydration reaction, weakens the crystallization of water, increases the viscosity of the solution to reduce the formation of ice crystals, and at the same time maintains a certain molar concentration inside and outside the cells to reduce the concentration of unfrozen solutions inside and outside the cells. The concentration of electrolytes in the medium protects cells ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 乔爱军
Owner 乔爱军
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