High-transparency PDA (potato dextrose agar) culture medium preparing method
A production method and high-transparency technology, applied in the application, organic fertilizer, inorganic fertilizer and other directions, can solve the problems of not reaching the standard amount, easy to stick to the pan, difficult to mix evenly, etc. less and improve the effect of proportioning accuracy
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] Embodiment one embodiment proposes a kind of preparation method of high transparency PDA culture medium, comprises the following steps:
[0018] A. Potatoes cut into cubes: 200g potatoes are washed, peeled, and bud eyes removed, and cut into cube cubes with a side length of 4-6mm;
[0019] B. Boiling cubes: Put the potato cubes in hot water, boil them on high heat for 5 minutes until softened, then change to slow fire for 10-14 minutes, so that the nutrients of the potatoes can be fully distributed into the water, and at the same time, the potatoes are cooked and not rotten;
[0020] C. Filtration: filter 2-3 times with 6-8 layers of gauze, and keep the filtrate;
[0021] D. Adding materials: Add 20g of glucose, 0.9g of potassium dihydrogen phosphate, 1.3g of magnesium sulfate, and 8g of edible grade salt to the above-mentioned filtrate, stir well, and dilute to 1000mL with water;
[0022] E, adjust the pH value: Use hydrochloric acid solution and sodium hydroxide solu...
Embodiment 2
[0027] The present embodiment proposes a kind of preparation method of high transparency PDA culture medium, comprises the following steps:
[0028] A. Potatoes cut into cubes: 200g potatoes are washed, peeled, and bud eyes removed, and cut into cube cubes with a side length of 4-6mm;
[0029] B. Boiling cubes: Put the potato cubes in hot water, boil them on high heat for 5 minutes until softened, then change to slow fire for 10-14 minutes, so that the nutrients of the potatoes can be fully distributed into the water, and at the same time, the potatoes are cooked and not rotten;
[0030] C. Filtration: filter 2-3 times with 6-8 layers of gauze, and keep the filtrate;
[0031] D. Adding materials: Add 20g of glucose, 1.0g of potassium dihydrogen phosphate, 1.4g of magnesium sulfate, and 10g of edible grade salt to the above-mentioned filtrate, stir well, and dilute to 1000mL with water;
[0032] E, adjust the pH value: Use hydrochloric acid solution and sodium hydroxide soluti...
Embodiment 3
[0037] The present embodiment proposes a kind of preparation method of high transparency PDA culture medium, comprises the following steps:
[0038] A. Potatoes cut into cubes: 200g potatoes are washed, peeled, and bud eyes removed, and cut into cube cubes with a side length of 4-6mm;
[0039] B. Boiling cubes: Put the potato cubes in hot water, boil them on high heat for 5 minutes until softened, then change to slow fire for 10-14 minutes, so that the nutrients of the potatoes can be fully distributed into the water, and at the same time, the potatoes are cooked and not rotten;
[0040] C. Filtration: filter 2-3 times with 6-8 layers of gauze, and keep the filtrate;
[0041] D. Adding materials: Add 20g of glucose, 1.2g of potassium dihydrogen phosphate, 1.6g of magnesium sulfate, and 12g of edible grade salt to the above-mentioned filtrate, stir well, and dilute to 1000mL with water;
[0042] E, adjust the pH value: Use hydrochloric acid solution and sodium hydroxide soluti...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com