High sensitivity detecting process for single-mode oxygen and super-oxygen anion

A superoxide anion, high-sensitivity technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problem of low concentration detection of singlet oxygen and superoxide anion, complex operation and high sensitivity Low-level problems, to achieve the effect of simple operation, simple measuring device and high sensitivity

Inactive Publication Date: 2003-10-15
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

[0008] The above methods have the following disadvantages to varying degrees: high-end instruments are required, which are difficult to achieve in general laboratories; low sensitivity, it is difficult to detect low concentrations of singlet oxygen and superoxide anion; the operation is complicated, and multiple instruments and drugs are used. Wait

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  • High sensitivity detecting process for single-mode oxygen and super-oxygen anion
  • High sensitivity detecting process for single-mode oxygen and super-oxygen anion
  • High sensitivity detecting process for single-mode oxygen and super-oxygen anion

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Embodiment Construction

[0023] The present invention will be further described below by specific examples.

[0024] 100ul of FCLA solution with a concentration of 10um, 100ul of HSA solution with a concentration of 5um, and 100ul of three-distilled water were mixed and placed in a measuring vessel. The oxygen concentration was measured to be 5.4mg / L by a dissolved oxygen analyzer, and then detected by bioluminescence. The system measures the chemiluminescence of the system and obtains figure 1 The measurement results of curve B in the middle. Dissolved oxygen in the FCLA-HSA system was removed by passing nitrogen. After passing nitrogen for 15sec, 30sec, 1min, 2min, 4min and 6min respectively, the remaining oxygen concentrations in the system were 2.9mg / L, 2.2mg / L and 1.6mg respectively. / L, 0.8mg / L, 0.4mg / L, 0.1mg / L. As the oxygen concentration in the system decreases, the luminous intensity also decreases, and the luminous attenuation trend is shown in Figure 4 .

[0025] as the picture shows,...

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Abstract

The present invention relates to test technology and is especially high-sensitivity detection of singlet state oxygen and superionic oxygen. Into 1-100 um/L concentration chemical luminous probe solution, albumin in 1-100 um/L concentration is added. The said method has the features of small amount of required specimen, simple operation, no need of expensive instrument and chemical, high sensitivity and low concentration detection capacity.

Description

(1) Technical field [0001] The invention relates to testing technology, in particular to a high-sensitivity detection method for singlet oxygen and superoxide anion. (2) Background technology [0002] Free radicals are necessary to maintain the normal physiological functions of the body. For example, they can participate in the signal transduction of biological cells, dilate blood vessels, kill invading microorganisms and attack diseased tumor cells and other normal physiological functions; however, excessive free radicals can also It will cause serious damage to the body, and the aging of organisms and the occurrence and development of many diseases are closely related to free radicals. Therefore, detecting the generation of free radicals in living organisms is of great significance to both basic research and medical clinical applications. [0003] At present, the commonly used free radical research methods mainly include the following: [0004] 1. Electron spin resonance...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 邢达郝敏
Owner SOUTH CHINA NORMAL UNIVERSITY
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