High sensitivity detecting process for single-mode oxygen and super-oxygen anion
A superoxide anion, high-sensitivity technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problem of low concentration detection of singlet oxygen and superoxide anion, complex operation and high sensitivity Low-level problems, to achieve the effect of simple operation, simple measuring device and high sensitivity
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[0023] The present invention will be further described below by specific examples.
[0024] 100ul of FCLA solution with a concentration of 10um, 100ul of HSA solution with a concentration of 5um, and 100ul of three-distilled water were mixed and placed in a measuring vessel. The oxygen concentration was measured to be 5.4mg / L by a dissolved oxygen analyzer, and then detected by bioluminescence. The system measures the chemiluminescence of the system and obtains figure 1 The measurement results of curve B in the middle. Dissolved oxygen in the FCLA-HSA system was removed by passing nitrogen. After passing nitrogen for 15sec, 30sec, 1min, 2min, 4min and 6min respectively, the remaining oxygen concentrations in the system were 2.9mg / L, 2.2mg / L and 1.6mg respectively. / L, 0.8mg / L, 0.4mg / L, 0.1mg / L. As the oxygen concentration in the system decreases, the luminous intensity also decreases, and the luminous attenuation trend is shown in Figure 4 .
[0025] as the picture shows,...
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