Method of transferring rhizobium japonicum to corn root system

A rhizobia, corn technology, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of inability to graft, no new varieties can be cultivated by grafting, etc., and achieve the effect of low cost and simple operation.

Inactive Publication Date: 2014-07-30
王孚望
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Problems solved by technology

At present, there is still a patented technology in China that uses the method of remote grafting to transfer rhizobia. The application number is 201010046310.9. This technology uses grafting to transfer rhizobia. When it grows to 6 leaves, after the seedling stage, it can no longer be grafted. In fact, if it has more than

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  • Method of transferring rhizobium japonicum to corn root system
  • Method of transferring rhizobium japonicum to corn root system
  • Method of transferring rhizobium japonicum to corn root system

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[0019] The present invention is described further below, and its specific implementation is as follows:

[0020] 1. Sowing:

[0021] Choose pure soybean seeds and pure corn seeds that are plump and flawless (transgenic soybeans and corn are not allowed), and sow them on-demand in the test field. The test field is suitable for sandy soil, with a humidity of 50%-60%, and a sowing depth of 2- 3 cm, covered with soil.

[0022] 2. Grafting:

[0023] ① Preparations before grafting: single-sided blades, medical alcohol (75%), non-static plastic strips (1 cm wide), stainless steel tweezers, bamboo needles (thick as a sewing needle, 1 cm long), sterile gloves. Sterile gloves must be worn during grafting, and all tools must be sterilized with alcohol, especially the bamboo needles must be sterilized and dried in advance and put into a sterile bag for later use.

[0024] ② Timing of grafting: The seedlings of corn and soybean have just been unearthed for 2-3 days. If corn only grows...

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Abstract

The invention relates to a cultivating technology in the crop field, and in particular to a method of transferring rhizobium japonicum to a corn root system with an ultra-distant compound type breeding technique. The method comprises the following steps: carrying out sexual propagation (hybridization) between corn and soybean; transferring dinitrogenase of the soybean to the corn, and enabling a root system of the grass family plant corn to form nodules. As the corn is ultra distant from the soybean, the corn and the soybean cannot be carried out with cross pollination, so at first, vegetative propagation (grafting) must be carried out, in order to close the genetic relationship and make the sexual propagation possible; the key of the compound breeding technique is firstly ensuring that the corn and the soybean are grafted successfully, in particular grated in the seedling phase, as the seedlings at this time communicate with each other most actively, and variation is most possible. But, the seedlings just come up out the ground are extremely fragile, and the plants are tender and small, so that the grafting is very difficult, and has not yet been successful in the foreign countries.

Description

technical field [0001] The invention relates to a breeding technology in the field of crops, in particular to a new method for transferring soybean rhizobia to corn roots for biological nitrogen fixation by using super-distant compound breeding technology, that is, through sexual reproduction of corn and soybeans, soybean The nitrogenase enzyme is transferred to corn, so that nodules can form on the root system of corn, but corn and soybean, which are super-distantly related, cannot be cross-pollinated. In order to make successful hybridization of corn and soybean, corn and soybean must first be asexually propagated , because asexual reproduction can increase the affinity between the two, and on the basis of improving the affinity of the two, the sexual reproduction can be successfully hybridized. The key to this compound breeding technique is to first ensure the successful grafting of corn and soybean. [0002] Background technique [0003] Biological science and technolog...

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Application Information

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IPC IPC(8): A01G1/00A01G1/06A01H1/02
Inventor 王孚望
Owner 王孚望
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