Lung cancer organ model and application thereof in tumor research

A technology of organoids and lung cancer, applied in lung cancer organoid models and its application in tumor research, can solve the problems of unpredictable curative effect, incomplete prediction of targeted therapy, etc., and achieve the effect of solving the problem of insufficient representation

Pending Publication Date: 2019-04-02
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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  • Abstract
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Problems solved by technology

However, genetic testing cannot predict the curative effect of chemotherapy and tra

Method used

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  • Lung cancer organ model and application thereof in tumor research
  • Lung cancer organ model and application thereof in tumor research
  • Lung cancer organ model and application thereof in tumor research

Examples

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Embodiment Construction

[0044] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0045] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0046]1. Main reagents: Australian solanine (MedChemExpress, #HY-N0069), Y-27632 (MedChemExpress, #HY-10071) Advanced DMEM / F-12 medium (Gibico, #12634028), McCoy's5a medium ( Gibico, #16600082), RPMI-1640 medium (Gibico, #11875093), F-12K medium (Gibico, #21127030), DMEM medium (Gibico, #11965118), phosphate buffered saline (Gibico, #14190144) , penicillin / streptomycin (10,000U / mL, Thermo Fisher Scientific, #15140122), fetal bovine serum (Gibico, #10100-147), L-glutamine (200mmol / L, Gibico, #25030164), hydroxyethyl Piperazine ethanesulfonic acid buffer (Gibico, #15630130), 0.25% trypsin-EDTA (Gibico, #25200114), N-2 addition solution (Gibico, #A1370701), B-27 addition solution (Gibico, #12587010 ); GF7 Supplement (Ketu Medicin...

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Abstract

The invention discloses a lung cancer organ model and an application thereof in tumor research. An establishment method of the lung cancer organ model comprises the steps as follows: (1) lung cancer tumor cells are added to a three-dimensional culture medium for culture, and the lung cancer tumor cells are added to a culture medium to be continuously cultured after a tumor layer is formed, whereinthe culture medium contains growth factors and extracellular matrix suitable for growth lung cancer tumor cells; (2) a system obtained through culture in step (1) forms single cell after protease enzymolysis, resuspension is performed by the culture medium after separation, and then, the lung cancer organ model is obtained. By use of the method, a specimen library containing a large number of lung cancer organ models can be established, heterogeneity and diversity of lung cancer are greatly covered, the defect of insufficient representativeness of traditional lung cancer cell lines is effectively overcome, and the lung cancer organ model becomes an efficient platform for research of occurrence and development mechanisms of lung cancer.

Description

technical field [0001] The invention relates to an organoid model and its application, in particular to a lung cancer organoid model and its application in tumor research. Background technique [0002] Lung cancer is a major disease that endangers the health of the Chinese people. The morbidity and mortality are as high as 53.37 / 100,000 and 45.57 / 100,000 respectively. Precision medicine treats lung cancer with precision and convenience. It uses gene sequencing platforms to detect cancer mutations. The selection of appropriate drugs largely avoids the blindness of patient treatment. However, genetic testing cannot predict the curative effect of chemotherapy and traditional Chinese medicine, and the prediction of targeted therapy is also imperfect. A predictive evaluation system that includes multiple systemic drug treatments is needed to improve precision treatment. [0003] Organoid culture is a cutting-edge technology for precise treatment of tumors in three-dimensional c...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02
CPCC12N5/0693C12N2503/02C12N2513/00G01N33/5011
Inventor 韩毅于建华高远冯利
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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