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This invention relates to a method for establishing and detecting the fingerprint spectrum of the traditional Chinese medicine composition Taohongyin. The raw materials of the traditional Chinese medicine composition include: chicken gizzard lining, papaya, hawthorn, chrysanthemum, tangerine peel, peach kernel, and wolfberry. The fingerprint spectrum establishment method includes the preparation of a reference solution and a test solution; detection of the reference solution and the test solution using high-performance liquid chromatography (HPLC); the mobile phase used in the HPLC is acetonitrile and phosphoric acid aqueous solution, and the elution method is gradient elution.