Macrophage polarization inhibitor and application thereof

A macrophage, inhibitor technology, applied in the field of macrophage polarization inhibitor

Inactive Publication Date: 2019-06-25
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no studies have reported the role of lncRNA in regulating DNA methylation modification mediated by Dnmt3a expression in the process of macrophage polarization.

Method used

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  • Macrophage polarization inhibitor and application thereof
  • Macrophage polarization inhibitor and application thereof
  • Macrophage polarization inhibitor and application thereof

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Embodiment Construction

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0028] refer to Figure 1-5 , a macrophage polarization inhibitor Dnmt3aos, its sequence number is shown in Table 1.

[0029] Table 1:

[0030]

[0031] A kit comprising the inhibitor Dnmt3aos according to claim 1.

[0032] An application of macrophage polarization inhibitor Dnmt3aos, comprising the following steps, the steps are as follows:

[0033] S1: M1 and M2 macrophages were prepared in vitro

[0034] Bone marrow cells were isolated from the femur and tibia of BALB / c mice, induced into BMDM by L929 conditioned medium, stimulated with LPS and IFN-γ to obtain M1, and stimulated with IL-4 to obtain M2 macrophages; then use Gene chip technology, select macro...

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Abstract

The invention relates to the field of biotechnology, in particular to a macrophage polarization inhibitor and application thereof. A macrophage polarization inhibitor Dnmt3aos, a kit containing the inhibitor Dnmt3aos claimed in Claim 1 and the application of the macrophage polarization inhibitor Dnmt3aos are provided. The Dnmt3aos plays a crucial role in macrophage polarization, Dnmt3aos-Dnmt3a axis-mediated DNA aberrant methylation may play a key role in the macrophage polarization, and the inhibitor enriches the understanding of the macrophage polarization mechanism and provides theoreticaldata support for studying the macrophage polarization.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a macrophage polarization inhibitor and application thereof. Background technique [0002] As an important component of the immune system, macrophages are ubiquitous in blood, lymph and tissues. They have obvious heterogeneity and plasticity. Under the influence of microenvironment in vivo and in vitro, they will be polarized into different phenotypes and exhibit different functions. . According to different activation methods and secreted cytokines, macrophages are mainly divided into two types: classically activated macrophages (M1) and alternatively activated macrophages (M2): M1 macrophages can be activated by lipopolysaccharide (LPS) ) and interferon gamma (IFN-γ) and granulocyte-macrophage colony stimulating factor (granulocyte-macrophage colony stimulating factor, GM-CSF) induced formation, through the release of high levels of pro-inflammatory factors such as interleukin-12 ...

Claims

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Application Information

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IPC IPC(8): C12N5/0786
Inventor 吕坤李雪琴杨辉
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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