35results about How to "Increase protein levels" patented technology

The invention provides application of a DNA (Deoxyribonucleic Acid) tetrahedron to preparation of a medicament for treating Alzheimer's disease. The DNA tetrahedron is assembled into a tetrahedron structure from four single-chain DNAs in a complementary base pairing manner; each edge of the tetrahedron is of a dual-chain DNA structure. The DNA tetrahedron provided by the invention can protect nerve cells in an amyloid-beta protein environment and restrain nerve cell apoptosis. The DNA tetrahedron has a very good application prospect in the preparation of the medicament for treating the Alzheimer's disease.

The present invention relates to an anti-CD66c antibody and its use for treating cancer, and more particularly, it is possible to induce T-cell activation or humoral immune response using an antibody specifically recognizing CD66c. A nucleic acid molecule encoding the antibody or antigen-binding fragment thereof, a vector comprising the nucleic acid molecule, a host cell and the antibody or antigen-binding fragment thereof is used for alleviation, prevention, treatment or diagnosis of CD66c-related disease.

The present invention provides a pyridine compound WXSA‑051B designed and synthesized against the enzyme active site to inhibit the enzyme activity of RBP2, and also provides its preparation method. The test proves that the compound can increase the H3K4me3 protein level in human gastric cancer cell BGC‑823 Significantly increased, it has obvious inhibitory effect on RBP2 enzyme activity, and can reduce the migration ability of human gastric cancer cell BGC‑823. The compound of the present invention can be used as a gastric cancer inhibitor to prepare anti-gastric cancer drugs. The compound structure is as follows.

The invention belongs to the field of biological medicine and particarly relates to application of miR-885-5p and miR-224-5p to medicament preparation. The invention verifies that under an in-vitro high inflammation environment, main pathogenic cell postorbital fibroblast of Graves eye disease directly generates resistance on glucocorticoid, but miR-885-5p and miR-224-5p can increase protein level of a glucocorticoid receptor in the postorbital fibroblast, can recover sensitivity of the postorbital fibroblast on glucocorticoid, can improve treatment effect of the glucocorticoid on Graves eye disease, so that theoretical basis and experimental data are provided for clinically treating Graves eye disease. A model consisting of baseline serum miR-885-5p, miR-224-5p, TRAb and palpebral fissure width can effectively predict a patient insensitive to glucocorticoid treatment.

The invention discloses novel application of echinatin. The echinatin and at least one of medically acceptable salt, ester, solvate, stereoisomer, tautomer and prodrug of the echinatin are applied to the product which has at least one of the following functions: (1) activating the signal path of Nrf2 and / or AMPK; (2) strengthening the expression of Nrf2, antioxidase and / or metabolic enzymes; (3) resisting aging; (4) inhibiting cell injury and tissue injury caused by oxidation; (5) protecting liver injury; (6) protecting chemical liver injury or oxidative liver injury; and (7) resisting vicious transformation of skin cells. The echinatin can be separated and purified from licorice root. The novel application of the echinatin provides a novel thought to researches and development of echinatin, and a series of novel products with the application are further provided.

The invention relates to human leukocyte protease inhibitory factor and its recombinant preparation and application, belonging to the field of biomedicine. The amino acid sequence contained in human leukocyte protease inhibitor is: (1) the amino acid sequence shown by SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3; (2) in SEQ ID NO: 1, SEQ ID NO: ID NO: 2 or the amino acid sequence obtained by connecting the N-terminal or / and C-terminal tag of the amino acid sequence shown in SEQ ID NO: 3; (3) in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 The amino acid sequence shown is substituted, deleted or added with one or several amino acids and has the activity of the human leukocyte protease inhibitor. The preparation method is to first construct the plasmid, then induce expression to obtain the fusion protein, and then perform renaturation and concentration after purification. The protein in the invention has important applications in the preparation of medicines for treating metabolic diseases, and can reduce body weight, inhibit the increase of fat tissue weight, and inhibit hepatic fatty degeneration.

The invention provides an ethanol extract of weeping tree, a preparation method thereof and an application of the ethanol extract of weeping bark in the preparation of anti-liver cancer drugs, belonging to the field of medicine. According to the preparation method of the alcohol extract of the weeping moss, the ethanol is used to extract the ethanol to obtain the ethanol extract of the weeping moss. The results of the examples show that the ethanol extract of weeping tree in the present invention inhibits the growth of liver cancer cells in a dose-dependent manner, and has no toxic effect on the spleen cells of mice; Activate the caspase signaling pathway and induce the apoptosis of liver cancer cells; the ethanol extract can also significantly increase the spleen index and the number of immune cells in mice, thereby improving the body's immunity and further improving the anti-tumor effect. The ethanol extract prepared by the invention has remarkable antitumor effect, is a pure natural component, is safe and non-toxic, and lays a foundation for the research and development of novel anti-liver cancer drugs.

The invention relates to protein feed rich in small peptides and multi-enzyme strains and a preparation method thereof. The protein feed is prepared by fermenting soybean meal, cottonseed meal, rapeseed meal, bone meal, wheat bran, common salt, light calcium carbonate, water and strains in a fermentation bin. The preparation method of the protein feed comprises the following steps: mixing the soybean meal, the cottonseed meal, the rapeseed meal, the bone meal, the wheat bran, the common salt and the light calcium carbonate in percentage by weight; adding 20-30 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer so as to increase the air pressure to 1-2 Mpa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin; inoculating 3-5 percent of strains when the temperature of the mixture falls to 30-37 DEG C; fermenting for 72 hours at the temperature of 32-37 DEG C; discharging from the bin; and bagging to obtain a finished product. The product has strong disease-resistant function and low cost, increases the production of animal by-products by more than 20 percent, is nontoxic, harmless and pollution-free and can inhibit food-borne diseases represented in cancers and subhealth phenomena from spreading.