34results about How to "Increase protein levels" patented technology

The invention relates to a novel veterinary antibacterial agent and in particular relates to a lauroyl arginine ethyl ester ion pair compound derivative which is prepared from lauroyl arginine ethyl ester LAE and an organic acid through reactions. As a livestock aquatic animal antibacterial agent, the derivative is capable of preventing and treating duckling riemerella anatipestifer diseases caused by riemerella anatipestifer, is capable of treating peritonitis diseases caused by escherichia coli and staphylococcus aureus, is capable of preventing and treating fish septicemia caused by aeromonas sobria, and has remarkably improved prevention and treatment effects when being compared with a conventional LAE veterinary antibacterial agent. The derivative can be completely degraded in human bodies and animal bodies and is small in toxic and side effect, environment drug resistance bacteria caused by residual antibacterial agents discharged into the environment can be avoided, and small adverse environment influence can be caused while an effective antibacterial effect is achieved.

The invention relates to protein feed rich in small peptides and multi-enzyme strains and a preparation method thereof. The protein feed is prepared by fermenting soybean meal, cottonseed meal, rapeseed meal, bone meal, wheat bran, common salt, light calcium carbonate, water and strains in a fermentation bin. The preparation method of the protein feed comprises the following steps: mixing the soybean meal, the cottonseed meal, the rapeseed meal, the bone meal, the wheat bran, the common salt and the light calcium carbonate in percentage by weight; adding 20-30 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer so as to increase the air pressure to 1-2 MPa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin; inoculating 3-5 percent of strains when the temperature of the mixture falls to 30-37 DEG C; fermenting for 72 hours at the temperature of 32-37 DEG C; discharging from the bin; and bagging to obtain a finished product. The product has strong disease-resistant function and low cost, increases the productionof animal by-products by more than 20 percent, is nontoxic, harmless and pollution-free and can inhibit food-borne diseases represented in cancers and subhealth phenomena from spreading.

The present invention provides wheat NAC nucleic acids and proteins that modulate grain protein content and senescence in plants. Overexpression of a NAC coding sequence can accelerate senescence and increase grain protein content and inhibition of expression can delay senescence. The invention also provides methods of using the nucleic acids to produce transgenic plants with altered grain protein content or senescence.

The invention discloses novel application of echinatin. The echinatin and at least one of medically acceptable salt, ester, solvate, stereoisomer, tautomer and prodrug of the echinatin are applied to the product which has at least one of the following functions: (1) activating the signal path of Nrf2 and/or AMPK; (2) strengthening the expression of Nrf2, antioxidase and/or metabolic enzymes; (3) resisting aging; (4) inhibiting cell injury and tissue injury caused by oxidation; (5) protecting liver injury; (6) protecting chemical liver injury or oxidative liver injury; and (7) resisting vicious transformation of skin cells. The echinatin can be separated and purified from licorice root. The novel application of the echinatin provides a novel thought to researches and development of echinatin, and a series of novel products with the application are further provided.

The invention belongs to the fields of biotechnology and medicine, and in particular relates to an application of an SET gene in preparing a product for diagnosing and/or treating gastric cancer. It is provided that the SET achieves frequent high expression (80%) in gastric cancer tissues, and the increase in SET expression is significantly correlated to aggressive phenotype and worse prognosis of a patient with the gastric cancer (P is less than 0.05). The low expression of the SET can obviously inhibit the migration and the invasion of gastric cancer cells. In addition, the SET, by virtue of a PP2A/Akt signaling pathway, can promote tumor malignant transformation. Furthermore, the gastric cancer tissues are obviously higher than para-carcinoma tissues in SET protein level. Therefore, the SET gene can serve as a specific marker gene for diagnosing the gastric cancer, so that it is more accurate and rapid to diagnose the gastric cancer. The SET gene provided by the invention provides a novel therapeutic target for the prevention and the treatment of the gastric cancer.

The invention relates to a special feed for mutton sheep. The special feed consists of the following ingredients in parts by weight: 5-15 parts of soybean meal, 10-30 parts of cottonseed meal, 5-20 parts of corn meal, 1-5 parts of a mutton sheep premix compound, 1-5 parts of calcium hydrophosphate, 1-4 parts of salt, 10-20 parts of an immunity-enhanced plant and 5-7 parts of a freeze-dried powder additive. In the ingredients of the feed disclosed by the invention, the soybean meal is a main protein raw material for preparing livestock and poultry, is rich in high protein and is combined with the cottonseed meal, the corn meal, the mutton sheep premix compound, the calcium hydrophosphate and the salt so that the feed is high in protein level, good in palatability and capable of obviously increasing the growth rate of the mutton sheep and increasing the feed conversion ratio; meanwhile, due to the immunity-enhanced plant, the use of drugs is reduced, so that drug residues and drug resistance are reduced, the production cost is lowered, the weight increment speed is increased, the disease resistance is improved, and the feed can be used as a special feed for the mutton sheep so as to produce pollution-free healthy sheep meat.

The invention relates to a breeder egg brooding and each-stage feeding method of spotted-brown chickens with week age of 50-59 weeks, belongs to the technical field of poultry culture and particularly relates to a brooding and feeding method of Huainan spotted-brown chickens. The scheme is as follows: the brooding of the spotted-brown chickens comprises a brooding early stage, a brooding middle stage and a brooding later stage; in the brooding early stage, chickens with the age of 1-14 days are cultured; in the brooding middle stage, the spotted-brown chickens with the age of 15-28 days are cultured; in the brooding later stage, the spotted-brown chickens with the age of 29-42 days are cultured. According to the method, a brooding environment condition is easy to control, heat energy is saved and disinfection is convenient to realize; padding and feeding cost are saved and the chickens are convenient to observe so that production management is easy to carry out; the survival rate of the spotted-brown chickens and the production benefits are improved.

The invention relates to a human leukocyte protease inhibitory factor as well as recombinant preparation and an application thereof, which belong to the field of biological medicines. The amino acid sequence contained in the human leukocyte protease inhibitory inhibitor is as follows: (1) an amino acid sequence shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3; (2) an amino acid sequence obtained by connecting a label to the N end or/and C end of the amino acid sequence shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3; and (3) an amino acid sequence which is obtained by substituting, deleting or adding one or more amino acids to the amino acid sequence shown in SEQ ID NO: 1 and SEQ ID NO: 2 or SEQ ID NO: 3 and has the activity of the human leukocyte protease inhibitory factor. The preparation method comprises the following steps: constructing plasmids, carrying out inducible expression to obtain fusion protein, purifying, and carrying out renaturation concentration. The protein provided by the invention has important application in preparation of drugs for treating metabolic diseases, and can reduce weight, inhibit increase of adipose tissue weight and inhibit liver fatty degeneration.

The invention discloses previously unknown modifiers of Aβ secretion. These proteins are identified as suitable targets for the development of new therapeutics to treat, prevent or ameliorate pathological conditions associated with Aβ secretion, including Alzheimer's Disease. The invention also relates to methods to treat, prevent or ameliorate said pathological conditions and pharmaceutical compositions therefore comprising modulators with inhibitory effects on the activity and/or expression of these modifiers.

The invention provides an alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver caner drugs, and belongs to the field of medicines. According to thepreparation method of the alcohol extract of rhytidium rugosum, the alcohol extract of rhytidium rugosum is obtained by extracting rhytidium rugosum with absolute ethyl alcohol. Results of embodiments show that the obtained alcohol extract of rhytidium rugosum inhibits the growth of liver cancer cells in a dose-dependent manner, and has no toxic effect on mouse spleen cells. The alcohol extract of rhytidium rugosum activates the caspase signal pathway through a mitochondrial pathway and a death receptor pathway to induce apoptosis of liver cancer cells. The alcohol extract of rhytidium rugosum can also significantly increase the spleen index and the number of immune cells in mice, so as to improve the body immunity and further improve the anti-tumor effect. The prepared alcohol extract ofrhytidium rugosum has a remarkable anti-tumor effect, contains pure natural components, is safe and non-toxic, and lays a foundation for research and development of novel anti-liver cancer drugs.

The invention belongs to the biomedical field, in particular to a pharmaceutical composition for treating and/or preventing myocardial ischemia-reperfusion injury and a preparation method thereof. Thepharmaceutical composition is chitosan nanoparticles coated with recombinant APN protein. The pharmaceutical composition of the invention is nanoparticles prepared from recombinant APN protein and chitosan, and the encapsulation efficiency can reach 81.9%; the nanoparticles were spheroid-like particles with particle size less than 700 nm and concentrated in the range of 100-500 nm. The pharmaceutical composition can improve left ventricular systolic and diastolic function of rats with myocardial ischemia-reperfusion injury and inhibit myocardial mitochondrial autophagy.

The invention provides a health-care oral liquid for delaying senescence and a preparation method thereof. A polypeptide and polysaccharide co-production preparation technology is adopted for the first time, sheep placenta freeze-dried powder, fructus lycii, flos carthami, longan aril and peach kernels serve as raw materials, a mixed solution rich in polypeptide (sheep placenta polypeptide) and polysaccharide (lycium barbarum polysaccharide and longan polysaccharide) is prepared, and after seasoning, the health-care oral liquid capable of delaying senescence can be prepared. In the health-care oral liquid for delaying senescence, the polypeptide content is 700-1900mg/100mL, the polysaccharide content is 200-2000mg/100mL, the hydroxysafflor yellow A content is 6-10mg/100mL, and the amygdalin content is 24-40mg/100mL, and the preparation can obviously improve the SIRT1 protein reduction condition caused by senescence, so that the effect of delaying senescence is achieved.