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Pharmaceutical composition for treating and/or preventing myocardial ischemia-reperfusion injury, and its preparation method

A technology for ischemia-reperfusion and reperfusion injury, which is applied in the field of biomedicine and can solve problems such as unclear treatment

Pending Publication Date: 2019-01-25
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether recombinant aminopeptidase N can be used as a new drug for the treatment of myocardial ischemia-reperfusion injury is still unclear

Method used

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  • Pharmaceutical composition for treating and/or preventing myocardial ischemia-reperfusion injury, and its preparation method
  • Pharmaceutical composition for treating and/or preventing myocardial ischemia-reperfusion injury, and its preparation method
  • Pharmaceutical composition for treating and/or preventing myocardial ischemia-reperfusion injury, and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A pharmaceutical composition for treating and / or preventing myocardial ischemia-reperfusion injury

[0038] The pharmaceutical composition is a chitosan nano particle loaded with recombinant APN protein.

[0039] The preparation method of the chitosan nanoparticle of described entrapment recombinant APN albumen, comprises the following steps:

[0040] (1) Dissolve 25 mg of chitosan with a molecular weight of 800 kDa in 8 mL of 10% glacial acetic acid solution, and adjust the pH to 6.5 with saturated sodium hydroxide solution to obtain solution I;

[0041] (2) Take 2 mL of recombinant APN solution with a concentration of 1.5 μmol / dL and 2 mL of 0.5 mg / mL sodium tripolyphosphate (TPP) aqueous solution and mix and stir (1000 rpm) evenly, and mix the recombinant APN and TPP solution with 1 drop / 3 Add it dropwise into the above solution I at a speed of ~5 seconds, and spontaneously form nanoparticles through the electrostatic interaction of anions and cations; obt...

Embodiment 2

[0042] Example 2 A pharmaceutical composition for treating and / or preventing myocardial ischemia-reperfusion injury

[0043] The pharmaceutical composition is a chitosan nano particle loaded with recombinant APN protein.

[0044] The preparation method of the chitosan nanoparticle of described entrapment recombinant APN albumen, comprises the following steps:

[0045] (1) Dissolve 14 mg of chitosan with a molecular weight of 200 kDa in 8 mL of 10% glacial acetic acid solution, and adjust the pH to 6.5 with saturated sodium hydroxide solution to obtain solution I;

[0046] (2) Take 2 mL of recombinant APN solution with a concentration of 1.5 μmol / dL and 2 mL of 0.5 mg / mL TPP aqueous solution and mix and stir (1000 rpm) evenly, and drop the mixed solution of recombinant APN and TPP at a speed of 1 drop / 3 to 5 seconds Add to the above solution I, spontaneously form nanoparticles through the electrostatic interaction of anions and cations; centrifuge, wash and freeze-dry. The su...

Embodiment 3

[0047] Example 3 A pharmaceutical composition for treating and / or preventing myocardial ischemia-reperfusion injury

[0048] The pharmaceutical composition is a chitosan nano particle loaded with recombinant APN protein.

[0049] The preparation method of the chitosan nanoparticle of described entrapment recombinant APN albumen, comprises the following steps:

[0050] (1) Dissolve 18 mg of chitosan with a molecular weight of 1500 kDa in 8 mL of 10% glacial acetic acid solution, and adjust the pH to 6.5 with saturated sodium hydroxide solution to obtain solution I;

[0051] (2) Take 2 mL of recombinant APN solution with a concentration of 1.5 μmol / dL and 2 mL of 0.5 mg / mL TPP aqueous solution and mix and stir (1000 rpm) evenly, and drop the mixed solution of recombinant APN and TPP at a speed of 1 drop / 3 to 5 seconds Add to the above solution I, spontaneously form nanoparticles through the electrostatic interaction of anions and cations; centrifuge, wash and freeze-dry. The s...

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Abstract

The invention belongs to the biomedical field, in particular to a pharmaceutical composition for treating and / or preventing myocardial ischemia-reperfusion injury and a preparation method thereof. Thepharmaceutical composition is chitosan nanoparticles coated with recombinant APN protein. The pharmaceutical composition of the invention is nanoparticles prepared from recombinant APN protein and chitosan, and the encapsulation efficiency can reach 81.9%; the nanoparticles were spheroid-like particles with particle size less than 700 nm and concentrated in the range of 100-500 nm. The pharmaceutical composition can improve left ventricular systolic and diastolic function of rats with myocardial ischemia-reperfusion injury and inhibit myocardial mitochondrial autophagy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a pharmaceutical composition for treating and / or preventing myocardial ischemia-reperfusion injury and a preparation method thereof. Background technique [0002] Cardiovascular disease is one of the diseases with the highest morbidity and mortality in my country, and acute myocardial infarction is a serious threat to national health due to its sudden onset, rapid progress, and high mortality. Reperfusion therapy such as thrombolysis and intervention has successfully saved the lives of many myocardial infarction patients by quickly opening the occluded coronary artery and restoring myocardial blood supply, and has become the first choice for the treatment of myocardial infarction. However, reperfusion therapy can also lead to myocardial ischemia-reperfusion injury, mainly manifested as arrhythmia, myocardial stunning, microvascular obstruction (no-reflow phenomenon) caused b...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61K9/50A61K47/36A61P9/10
CPCA61K9/5036A61K38/4886A61P9/10
Inventor 郝盼盼刘燕萍
Owner SHANDONG UNIV QILU HOSPITAL
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