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Ethanol extract of weeping tree and its preparation method and application of ethanol extract in preparing anti-liver cancer drugs

A technique for the preparation of anti-liver cancer drugs, the alcohol extract of Physcomitrhiza and its preparation field, which can solve the problem of incompetence or low anti-tumor immunity, easy drug resistance, and prognosis of patients. Very poor and other problems, to achieve significant anti-tumor effect, improve anti-tumor effect, and improve the level of effect

Active Publication Date: 2021-11-30
XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The small molecule targeted therapy drugs Sorafenib and Regrafenib are currently FDA-approved standard treatment methods for advanced liver cancer. Sorafenib is currently the only standard first-line systemic drug available for advanced liver cancer, but the median survival time is only 12.3 months; regrafenib is a second-line systemic drug, the median survival time is only 10.6 months; although sorafenib and regrafenib can improve the overall survival rate of patients with liver cancer, but the time is short, Drug resistance is easy to develop, and the prognosis is still poor. Patients with incompetent or low anti-tumor immunity are prone to relapse

Method used

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  • Ethanol extract of weeping tree and its preparation method and application of ethanol extract in preparing anti-liver cancer drugs
  • Ethanol extract of weeping tree and its preparation method and application of ethanol extract in preparing anti-liver cancer drugs
  • Ethanol extract of weeping tree and its preparation method and application of ethanol extract in preparing anti-liver cancer drugs

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preparation example Construction

[0030] The present invention provides a method of preparation of perimeter, including the following steps:

[0031] Vertific waves were extracted with anhydrous ethanol, and the vitrome ratio of the vitrifulse of the vitrome and anhydrous ethanol was 1 g: (10 ~ 20 mL).

[0032] The invention is extracted by anhydrous ethanol to obtain a vitro-granol. In the present invention, the perimeter moss is preferably a van powder, which can make the effective ingredients in the vitrogen in the validity of the vitrogen, improve the effective ingredient of the perimeter extract. The effect of improving the effect of peppermented petroleum cancer cells. The invention is extracted by anhydrous ethanol, the mass ratio of the peripheral boss and anhydrous ethanol is 1 g: (10 to 20 mL), more preferably 50 to 60 ° C, more preferably 60 ° C. . In the present invention, the vertical branch is preferably extracted in the form of a powder, which is advantageous for dissolution of the active material. ...

Embodiment 1

[0044] Preparation of perimeter

[0045] Vertic branch is collected in Xinjiang Markic Forest Farm and Urumqi Nanshan Small Canal Forest Farm, taking dry vulva, pulverized to obtain powder; pelletting powder and anhydrous ethanol according to a mass volume ratio of 1 g: 20ml at 60 ° C water bath After refluxing extraction 2H; then centrifuge under 8000 rpm was centrifuged at 8,000 rpm, taking a clear, and the precipitation continued to reflux 3 times. Centrifugation, combined supernatant; filtration after the combined supernatant, the filtrate was evaporated to mix to no oriented, dried, and Rhytidium Rugosum Ethanol Extract, RREE. RREE was dissolved with dimethyl sulfoxide (DMSO), and the polysaccharide content was measured by phenol-sulfuric acid, and the polysaccharide mass was 28.9%; the flavonoid content was determined by alkaline method, and its flavonoids The percentage content was 4.6%.

[0046] Phenol - Sulfuric acid method Reference is: Dubois M, Gilles Ka, Hamilton J K,...

Embodiment 2

[0049] Preparation of perimeter

[0050] The preparation process is in Example 1. Different in: the perimeter powder and anhydrous ethanol are extracted from a mass volume ratio of 1 g: 10 ml at 60 ° C water bath; then centrifuge at 6000 rpm for 20 min, take the supernatant , Precipitation continues to reflux 4 times.

[0051] After the percentage of the peel lever was dissolved by DMSO, the concentration was 30 mg / ml; the polysaccharide mass was 25.4%; the mass content of flavonoids was 4.1%.

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Abstract

The invention provides an ethanol extract of weeping tree, a preparation method thereof and an application of the ethanol extract of weeping bark in the preparation of anti-liver cancer drugs, belonging to the field of medicine. According to the preparation method of the alcohol extract of the weeping moss, the ethanol is used to extract the ethanol to obtain the ethanol extract of the weeping moss. The results of the examples show that the ethanol extract of weeping tree in the present invention inhibits the growth of liver cancer cells in a dose-dependent manner, and has no toxic effect on the spleen cells of mice; Activate the caspase signaling pathway and induce the apoptosis of liver cancer cells; the ethanol extract can also significantly increase the spleen index and the number of immune cells in mice, thereby improving the body's immunity and further improving the anti-tumor effect. The ethanol extract prepared by the invention has remarkable antitumor effect, is a pure natural component, is safe and non-toxic, and lays a foundation for the research and development of novel anti-liver cancer drugs.

Description

Technical field [0001] The present invention belongs to the field of medicine, and is specifically related to the use of perimeter extracts and preparation methods and the use of pendant alcoholin in the preparation of anti-liver cancer drugs. Background technique [0002] Liver cancer is one of the incidence and malignant tumors of high incidence and high prognosis, mainly including primary liver cancer and secondary liver cancer, hepatocellular carcinoma (HCC) accounting for 90% of primary liver cancer. The incidence and death rate of liver cancer and death in malignant tumors are high, and the morbidity of male liver cancer is rampted in the third place of malignant tumors, the mortality is high; the incidence of female liver cancer and mortality are ranked in malignant tumors. Seventh and third place. With the continuous development of science and technology, progress is currently made in the prevention and diagnosis of liver cancer. In terms of hepatoma, chemotherapy, surgic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/10A61P1/16A61P35/00A61P37/04
CPCA61K36/10A61P1/16A61P35/00A61P37/04
Inventor 李金耀马荣斌周方方
Owner XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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