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A kind of water-soluble conjugated polymer/graphyne nanoparticle and its preparation method and application

A conjugated polymer and nanoparticle technology, used in wave energy or particle radiation treatment materials, drug combinations, pharmaceutical formulations, etc., can solve the problem that photodynamic therapy cannot provide active oxygen, the release efficiency requires multiple injections, and gas therapy limit and other issues

Active Publication Date: 2022-04-12
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low oxygen level in the hypoxic state, it is impossible to continuously provide sufficient oxygen for photodynamic therapy to generate reactive oxygen species, which limits the development of photodynamic therapy for cancer.
In addition, gas therapy is generally limited by drug loading capacity, release efficiency, and the need for multiple injections.

Method used

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  • A kind of water-soluble conjugated polymer/graphyne nanoparticle and its preparation method and application
  • A kind of water-soluble conjugated polymer/graphyne nanoparticle and its preparation method and application
  • A kind of water-soluble conjugated polymer/graphyne nanoparticle and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, preparation water-soluble conjugated polymer / graphyne nanoparticle

[0074] 1. Preparation

[0075] according to figure 2 The flow chart shown prepares water-soluble conjugated polymer / graphyne nanoparticles, and the specific steps are as follows:

[0076] (1) Disperse 3 mg of graphdiyne powder in 1 mL of deionized water and sonicate for 4 h, add 0.75 mL of CPs DMSO solution (PBF or PFP, 4 mg / mL) and continue sonicating for 1 h. The interacting polymers were removed by ultrafiltration and centrifugation and washed three times with deionized water to obtain CPs@GDY.

[0077] (2) Add 15 mL of DSPE-PEG2000 (20 mg / mL) aqueous solution and sonicate for 4 h, remove excess DSPE-PEG2000 by ultrafiltration and centrifugation and wash with deionized water three times, and the purified CPs@GDY / DSPE-PEG is concentrated to 6mL, the concentration of CPs@GDY / DSPE-PEG is 0.5mg / mL.

[0078] 2. Representation

[0079] (1) Absorption and fluorescence spectra of conjuga...

Embodiment 2、 application example

[0093] 4T1 cells were purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, catalog number 3131C0001000800032.

[0094] RPMI 1640 culture medium was purchased from thermalfisher, product number 22400105.

[0095] 1. Intracellular ATP content level test

[0096] Take 8×10 4 / mL 4T1 cells were seeded in a six-well plate and cultured for 24 hours to allow the cells to adhere to the wall (4T1 cells were cultured in RPMI 1640 medium containing 10% FBS in 5% CO 2 , in a cell culture incubator at 37 °C. The cells were digested with 0.25% trypsin for 2 minutes and subcultured at a ratio of 1:4. ). Discard the supernatant medium and replace it with a cell culture medium containing 5 μg / mL CPs@GDY / DSPE-PEG and 1 mM TEOA and incubate overnight. Discard the supernatant, wash three times with PBS, replace with cell culture medium containing 1 mM TEOA, and place the cells in 5% CO 2 , 1%O 2 , and continue to incubate for 6 ...

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Abstract

The invention discloses a water-soluble conjugated polymer / graphyne nanoparticle, a preparation method and application thereof. The water-soluble conjugated polymer / graphyne nanoparticles of the present invention are self-assembled from graphyne, conjugated polymers and amphiphilic molecules; the conjugated polymer is a polymer shown in formula I or formula II : the amphiphilic molecule is distearoylphosphatidylethanolamine-polyethylene glycol. The water-soluble conjugated polymer / graphyne nanoparticles of the present invention can be photoregulated and catalyzed to reduce carbon dioxide into carbon monoxide in an anaerobic environment, and act on intracellular mitochondria to reduce the intracellular ATP level, reduce the membrane potential of mitochondria, and affect cells. Normal metabolic function, induced apoptosis of hypoxic cancer cells. The invention plays an important role in the development of the catalytic system of the functionalized nanoparticle for the treatment of solid tumors under hypoxic conditions.

Description

technical field [0001] The invention belongs to the technical field of organic synthesis and biomedicine, and in particular relates to a water-soluble conjugated polymer / graphyne nanoparticle and a preparation method and application thereof. Background technique [0002] The hypoxic state in the tumor is mainly due to the rapid proliferation and metabolism of a large number of cells, abnormal blood vessels in the tumor, increased pressure in the tumor, abnormal energy consumption, etc., which consume a large amount of oxygen, and it is difficult for the external ambient gas to diffuse into the interior, resulting in a decrease in the local oxygen content. Product CO 2 level up. The tumor microenvironment under hypoxia often exhibits high reactive oxygen species levels, low pH, and abnormal metabolic levels compared with cells normally under normoxia. As a hypoxic environment leads to tumor cell heterogeneity and suppression of innate adaptive immune responses, it increases...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/34A61K47/59A61P35/00
CPCA61K41/0057A61K9/5146A61K47/59A61P35/00
Inventor 王树张恩东刘礼兵吕凤婷黄一鸣
Owner INST OF CHEM CHINESE ACAD OF SCI
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