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Alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver cancer drugs

A technology of Physcomitrhiza and alcohol extract, which is applied in the application field of Physcomitrella ethanol extract and its preparation, and the preparation of anti-liver cancer drugs, which can solve the problem of incompetent or low anti-tumor immunity, short timeliness, and easy drug resistance in patients sexual issues

Active Publication Date: 2021-01-01
XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The small molecule targeted therapy drugs Sorafenib and Regrafenib are currently FDA-approved standard treatment methods for advanced liver cancer. Sorafenib is currently the only standard first-line systemic drug available for advanced liver cancer, but the median survival time is only 12.3 months; regrafenib is a second-line systemic drug, the median survival time is only 10.6 months; although sorafenib and regrafenib can improve the overall survival rate of patients with liver cancer, but the time is short, Drug resistance is easy to develop, and the prognosis is still poor. Patients with incompetent or low anti-tumor immunity are prone to relapse

Method used

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  • Alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver cancer drugs
  • Alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver cancer drugs
  • Alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver cancer drugs

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preparation example Construction

[0030] The invention provides a kind of preparation method of ethanol extract, comprising the steps of:

[0031] Adopt absolute ethanol to extract Physcomitrella, obtain the alcoholic extract of Dendrophyllum; the volume ratio of the quality of described Dendrophyllum and absolute ethanol is 1g: (10~20ml).

[0032] The invention adopts absolute ethanol to extract the weeping moss to obtain the weeping moss ethanol extract. In the present invention, the moss is preferably the moss powder, and the moss powder can be used as the raw material for extraction to release the active ingredients in the moss more fully, and improve the active ingredient of the moss ethanol extract. Content, improve the anti-hepatocarcinoma effect of ethanol extract. The present invention adopts absolute ethanol to extract the weeping moss, the volume ratio of the mass of the weeping moss and absolute ethanol is 1g: (10 ~ 20ml), more preferably 50 ~ 60 ℃, more preferably 60 ℃ . In the present inventio...

Embodiment 1

[0044] Preparation of Alcohol Extract of Pterodactylus

[0045] The weeping moss was collected from Xinjiang Mulei Forest Farm and Urumqi Nanshan Xiaoquzi Forest Farm, and the dried weeping moss plant was taken and crushed to obtain powder; the weight and volume ratio of weeping moss powder and absolute ethanol was 1g: 20ml in a water bath at 60°C Reflux extraction for 2 hours; then the extract was centrifuged at 8000 rpm for 15 minutes, the supernatant was taken, and the precipitate was refluxed for 3 times. Centrifuge and combine the supernatants; filter the combined supernatants, concentrate the filtrate by rotary evaporation until it has no ethanol smell, and dry it to obtain Rhytidium rugosum ethanol extract (RREE). Dissolve RREE with dimethyl sulfoxide (DMSO) at a concentration of 30 mg / ml. The polysaccharide content is determined by the phenol-sulfuric acid method, and the polysaccharide mass percentage is 28.9%; the flavone content is determined by the alkaline method,...

Embodiment 2

[0049] Preparation of Alcohol Extract of Pterodactylus

[0050] The preparation process is the same as in Example 1, the difference is that the moss powder and absolute ethanol have a mass volume ratio of 1g: 10ml, and reflux extraction in a water bath at 60°C for 3h; then the extract is centrifuged at 6000rpm for 20min, and the supernatant is taken , and the precipitate was continuously refluxed and extracted 4 times.

[0051] After being dissolved in DMSO, the obtained ethanol extract has a concentration of 30 mg / ml; the mass percentage of polysaccharides is 25.4%; the mass percentage of flavonoids is 4.1%.

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Abstract

The invention provides an alcohol extract of rhytidium rugosum, its preparation method and application in preparation of anti-liver caner drugs, and belongs to the field of medicines. According to thepreparation method of the alcohol extract of rhytidium rugosum, the alcohol extract of rhytidium rugosum is obtained by extracting rhytidium rugosum with absolute ethyl alcohol. Results of embodiments show that the obtained alcohol extract of rhytidium rugosum inhibits the growth of liver cancer cells in a dose-dependent manner, and has no toxic effect on mouse spleen cells. The alcohol extract of rhytidium rugosum activates the caspase signal pathway through a mitochondrial pathway and a death receptor pathway to induce apoptosis of liver cancer cells. The alcohol extract of rhytidium rugosum can also significantly increase the spleen index and the number of immune cells in mice, so as to improve the body immunity and further improve the anti-tumor effect. The prepared alcohol extract ofrhytidium rugosum has a remarkable anti-tumor effect, contains pure natural components, is safe and non-toxic, and lays a foundation for research and development of novel anti-liver cancer drugs.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an ethanol extract of weeping tree, a preparation method thereof and an application of the ethanol ethanol extract in preparing anti-liver cancer drugs. Background technique [0002] Liver cancer is one of the malignant tumors with high morbidity and mortality and extremely poor prognosis. It mainly includes primary liver cancer and secondary liver cancer. Hepatocellular carcinoma (HCC) accounts for about 90% of primary liver cancer. Among malignant tumors, the incidence and death cases of liver cancer are relatively high. The incidence of male liver cancer ranks third among malignant tumors, and the mortality rate ranks second among malignant tumors; the incidence and mortality of female liver cancer rank first among malignant tumors. seventh and third place. With the continuous development of science and technology, progress has been made in the prevention and diagnosis of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/10A61P1/16A61P35/00A61P37/04
CPCA61K36/10A61P1/16A61P35/00A61P37/04
Inventor 李金耀马荣斌周方方
Owner XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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