Application of mir‑885‑5p and mir‑224‑5p in drug preparation

A drug and applied technology, applied in the field of biomedicine, to achieve the effect of improving the therapeutic effect

Active Publication Date: 2017-03-29
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether miRNAs are involved in GC resistance in GO patients, and how they are involved in GC resistance through pathogenic cells OF

Method used

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  • Application of mir‑885‑5p and mir‑224‑5p in drug preparation
  • Application of mir‑885‑5p and mir‑224‑5p in drug preparation
  • Application of mir‑885‑5p and mir‑224‑5p in drug preparation

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Experimental program
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Embodiment 1

[0021] 1. The resistance test of primary OF cells to glucocorticoids under the stimulation of high concentration of inflammatory factors

[0022] In the present invention, the primary OF cells of 3 severe GO patients are stimulated by two concentrations (5ng / ml and 20ng / ml) of TNFα under the basic state to simulate the local inflammatory environment of different degrees, and the culture supernatant of the detection condition The concentration of sICAM-1, a cytokine related to pathogenicity and curative effect in GO. Compared with the basal state, stimulation of 5ng / ml TNFα can increase the secretion of sICAM-1, and 20ng / ml no longer significantly increase the concentration of sICAM-1. However, after treatment with 1 μM dexamethasone (Dex), the inhibitory effect on sICAM-1 secretion stimulated by 20 ng / ml TNFɑ decreased from 69% at 5 ng / ml to 28%, suggesting resistance to Dex.

[0023] 2. Validation of efficacy-related miRNAs

[0024] (1) Screening of efficacy-related miRNAs ...

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Abstract

The invention belongs to the field of biological medicine and particarly relates to application of miR-885-5p and miR-224-5p to medicament preparation. The invention verifies that under an in-vitro high inflammation environment, main pathogenic cell postorbital fibroblast of Graves eye disease directly generates resistance on glucocorticoid, but miR-885-5p and miR-224-5p can increase protein level of a glucocorticoid receptor in the postorbital fibroblast, can recover sensitivity of the postorbital fibroblast on glucocorticoid, can improve treatment effect of the glucocorticoid on Graves eye disease, so that theoretical basis and experimental data are provided for clinically treating Graves eye disease. A model consisting of baseline serum miR-885-5p, miR-224-5p, TRAb and palpebral fissure width can effectively predict a patient insensitive to glucocorticoid treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of miR-885-5p and miR-224-5p in medicine preparation. Background technique [0002] Graves' ophthalmopathy (GO) is the most prominent extrathyroidal lesion of Graves' disease (GD). In 2013, Tanda et al. found that 26% of GD patients were accompanied by GO in 346 patients with initial hyperthyroidism according to EUGOGO criteria. Retroorbital fibroblasts (OF) are pathogenic antigen carriers and effector cells of GO. Different from fibroblasts in other parts, in addition to OF-specific antigens such as IGFR-1R, OF also expresses thyroid cell-specific expression molecules, such as TSHR, Tg, TPO and NIS, which is called the simulated reconstruction of the thyroid gland in the orbit. Therefore, the circulating antibodies against thyroid in GD patients also attack OF, together with other antibodies against OF, stimulate their proliferation, differentiation into fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P27/02A61P5/44
Inventor 宁光沈力韵叶蕾王卫庆洪洁朱巍蔡洁黄凤娇
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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