Uses of Licochalcone

A medical and stereoisomer technology, applied in the field of licorice chalcone, can solve the problems of limited use, high price, serious side effects, etc., to increase the level of GSH, reduce the area of ​​liver necrosis, and reduce the level of ALT and AST Effect

Active Publication Date: 2019-01-08
PEKING UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are various kinds of hepatoprotective drugs clinically, but most of them are expensive or have relatively large toxic and side effects, so that their use is limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uses of Licochalcone
  • Uses of Licochalcone
  • Uses of Licochalcone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, the activating effect of licorice chalcone on Nrf2

[0058] In this example, the effect of licorice chalcone on the viability of HepG2 cells and the activity of Nrf2 / ARE reporter gene was investigated.

[0059] 1. Experimental materials and methods

[0060] (1) Glycyrrhizalcone was purchased from Chengdu Mansite Biotechnology Co., Ltd., and its H-NMR and C-NMR spectra were as follows: figure 1 and figure 2 shown. The human liver cancer cell line HepG2 was purchased from the American Type Culture Collection (ATCC), and the cells in the logarithmic growth phase were used in the experiments.

[0061] HepG2 cells were stably transfected with Nrf2 luciferase reporter gene using Lipofectamine 2000 (Life Technologies), and the resulting cells were called HepG2C8 cells. After HepG2C8 cells were seeded in 24-well plates for 12 hours, licochalcone of specified concentration was added respectively, the cells were lysed after 6 hours, the luciferase activity was ...

Embodiment 2

[0066] Example 2, the application of licorice chalcone in the preparation of products that activate Nrf2 and / or AMPK signaling pathways, antioxidant enzyme inducers and anti-aging products

[0067] 1. Experimental materials and methods

[0068] (1) MEFs cells were obtained from C57 mouse embryos at 13.5 days of pregnancy (Mingyu Chen, Experimental Animal Science). After MEFs cells in the logarithmic growth phase were inoculated in 6-well plates for 24 hours, licochalcone of specified concentration was added respectively, and culture was continued for 6 hours or specified time. The supernatant was discarded, and the cells were lysed with TRIZOL buffer (Beijing Quanshijin Biotechnology Co., Ltd.) to extract total RNA. Then qPCR method was used to detect the mRNA levels of HO-1 and NQO-1.

[0069] (2) 24 hours after the MEFs cells were seeded in a 60mm dish, licochalcone of specified concentration was added respectively, and the culture was continued for 6 hours or specified ti...

Embodiment 3

[0084] Example 3, Application of Glycyrrhizalcone in the Preparation of Antioxidant Enzyme Inducers and Anti-Skin Cancer Cell Transformation Products

[0085] 1. Experimental materials and methods

[0086] (1) JB6P+ cells in the logarithmic growth phase (ATCC) were inoculated on a 6-well plate for 24 hours, then licorice chalcones of specified concentrations were added, and the culture was continued for 72 hours. The supernatant was discarded, and the cells were lysed with TRIZOL buffer to extract total RNA. Then the mRNA levels of Nrf2, HO-1, Gclc and NQO-1 were detected by qPCR method.

[0087] (2) 24 hours after the JB6P+ cells were inoculated in the 6-well plate, licochalcone of the specified concentration was added respectively, and the culture was continued for 72 hours. The supernatant was discarded, the cells were lysed with RIPA buffer, and the protein concentration was determined by BCA method. 20 μg of protein from each sample was separated by SDS-polyacrylamide ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses novel application of echinatin. The echinatin and at least one of medically acceptable salt, ester, solvate, stereoisomer, tautomer and prodrug of the echinatin are applied to the product which has at least one of the following functions: (1) activating the signal path of Nrf2 and / or AMPK; (2) strengthening the expression of Nrf2, antioxidase and / or metabolic enzymes; (3) resisting aging; (4) inhibiting cell injury and tissue injury caused by oxidation; (5) protecting liver injury; (6) protecting chemical liver injury or oxidative liver injury; and (7) resisting vicious transformation of skin cells. The echinatin can be separated and purified from licorice root. The novel application of the echinatin provides a novel thought to researches and development of echinatin, and a series of novel products with the application are further provided.

Description

technical field [0001] The present invention relates to the new application of licorice chalcone. Background technique [0002] Nrf2 is a core regulator of phase II detoxification or antioxidant enzymes that protect cells from oxidative damage. The activation of Nrf2 can enhance the body's antioxidant capacity and body's ability to detoxify. The use of Nrf2 agonists has been shown to play a protective role to varying degrees in a variety of diseases. For example, SFN is a compound extracted from broccoli and cabbage. Many literatures have reported the protective effect of SFN on diabetes, cardiovascular disease and neurodegenerative diseases. Studies have shown that Nrf2 loss can aggravate the severity of various diseases. For example, compared with the control group, after knocking out Nrf2 in the mouse islet β cell line MIN6β cells, the damage caused by hydrogen peroxide, including biological indicators such as cell viability, cell morphology, and apoptosis, were signi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A61P39/06A61P1/16A23L33/10A61K8/35A61Q19/00
Inventor 叶敏余四旺王永瑞季帅乔雪唐叔南李紫薇
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap