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Mesenchymal stem cell preparation for treating diabetic cutaneous ulcers and preparation method of mesenchymal stem cell preparation

A technology for mesenchymal stem cells and skin ulcers, applied in the field of mesenchymal stem cell preparations for the treatment of diabetic skin ulcers and their preparation, can solve problems such as unsatisfactory effects, prolong the residence time, promote the formation of new blood vessels, and regulate macrophages The effect of cell polarization

Pending Publication Date: 2021-12-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN104248644A provides a compound preparation containing temperature-sensitive hydrogel and bone marrow mesenchymal stem cells, which can promote the repair of diabetic skin wounds, but the effect is not very satisfactory, and the average unhealed area of ​​the wound after use is still up to around 25%

Method used

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  • Mesenchymal stem cell preparation for treating diabetic cutaneous ulcers and preparation method of mesenchymal stem cell preparation
  • Mesenchymal stem cell preparation for treating diabetic cutaneous ulcers and preparation method of mesenchymal stem cell preparation
  • Mesenchymal stem cell preparation for treating diabetic cutaneous ulcers and preparation method of mesenchymal stem cell preparation

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Effect test

Embodiment 1

[0038] The preparation of embodiment 1 mesenchymal stem cell preparation

[0039] Mix sodium carboxymethylcellulose and pectin at a molar ratio of 90:1 to obtain 1 mL of hydrocolloid gel (HydroactiveGel), then dilute Hydroactive Gel to 5 times volume and 10 times volume respectively with the complete medium of WJMSCs, Then carry umbilical cord mesenchymal stem cells (concentration: 5×10 6 ~2×10 7 cells / mL) to obtain mesenchymal stem cell preparations. for the following experiments.

Embodiment 2

[0040] Example 2 Effect of Hydroactive Gel on WJMSCs

[0041] (1) Effect of Hydroactive Gel diluted in different proportions on WJMSCs

[0042] Use Live / Dead TM Cell Imaging Kit detects the survival of WJMSCs in mesenchymal stem cell preparations ( figure 1 A), the results showed that the survival of WJMSCs did not change significantly under the 5-fold and 10-fold dilution ratio of Hydroactive Gel; the cell viability of WJMSCs in separate WJMSCs and mesenchymal stem cell preparations were detected by CCK-8 ( figure 1 B), the results show that the cell viability of WJMSCs has no significant change under the 5-fold and 10-fold dilution ratio of Hydroactive Gel. It shows that diluting Hydroactive Gel to 5-10 times the volume has no significant effect on the survival and cell activity of WJMSCs, and has no toxicity to WJMSCs, and is suitable for loading WJMSCs. In addition, compared with HydroactiveGel after 10 times volume dilution, the mesenchymal stem cell preparation obtai...

Embodiment 3

[0056] Example 3 Therapeutic Effect of Mesenchymal Stem Cell Preparation on Diabetic Skin Ulcer

[0057] (1) Effect of mesenchymal stem cell preparation on skin wound healing

[0058] After feeding SD rats with a high-sugar and high-fat diet for 4 weeks, the rat model of type 2 diabetes was constructed by intraperitoneal injection of streptozotocin (STZ). Figure 5 As shown in A, PBS, Hydroactive Gel, WJMSCs (quantity is 2×10 6 ), mesenchymal stem cell preparation (the number of WJMSCs is 2×10 6 , the concentration is 2×10 7 individual / mL) and WJMSCs (the number is 1×10 7 1) were transplanted to the skin wounds of diabetic rats, and then the wound healing was checked on the 5th, 7th, 10th and 14th days after transplantation. The overall results were as follows: Figure 5 Shown in B, wherein, the wound healing rate of the 7th day is as follows Figure 5 As shown in C, the wound healing rate on the 14th day was as follows Figure 5 D shows.

[0059] From Figure 5 B, it ...

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Abstract

The invention provides a mesenchymal stem cell preparation for treating diabetic cutaneous ulcers and a preparation method of the mesenchymal stem cell preparation. The mesenchymal stem cell preparation comprises hydrocolloid gel and mesenchymal stem cells, wherein the hydrocolloid gel comprises sodium carboxymethyl cellulose and pectin, and the molar ratio of the sodium carboxymethyl cellulose to the pectin is (80-100): 1. According to the mesenchymal stem cell preparation, the specific hydrocolloid gel is adopted to carry the mesenchymal stem cells, so that the residence time of the mesenchymal stem cells in a wound part is effectively prolonged, implantation of the mesenchymal stem cells is promoted, the wound healing rate of the diabetic cutaneous ulcers is remarkably increased, the mesenchymal stem cell preparation effectively promotes skin tissue regeneration, promotes new angiogenesis and regulates macrophage polarization, which indicates that the mesenchymal stem cell preparation disclosed by the invention is suitable for preventing and / or treating the diabetic cutaneous ulcers.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to a mesenchymal stem cell preparation for treating diabetic skin ulcers and a preparation method thereof. Background technique [0002] Diabetic cutaneous ulcers (Diabetic cutaneous ulcers, DCU) refer to diabetic patients due to long-term poor blood sugar control, which leads to neuropathy and various degrees of peripheral vascular lesions, resulting in infection of lower extremities, ulcer formation and destruction of deep tissues. Diabetic skin ulcers are ulcers and gangrene that occur in diabetic patients due to microcirculatory disturbance caused by neurovascular lesions. The body of diabetic patients has been in a high-glucose environment for a long time, resulting in insufficient endogenous nitric oxide. In the high-glucose environment, the leukocyte function is defective, resulting in insufficient immune response. At the same time, the lack of new blood ve...

Claims

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Application Information

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IPC IPC(8): A61K47/38A61K47/36A61K35/28A61P17/02A61P3/10
CPCA61K47/38A61K47/36A61K35/28A61P17/02A61P3/10
Inventor 黄军就焦义仁
Owner SUN YAT SEN UNIV
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