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Method for researching influence on macrophage polarization by PPARs

A technology that affects macrophages and polarization, applied in biochemical equipment and methods, pharmaceutical formulations, compound screening/testing, etc., can solve problems that are poorly understood

Inactive Publication Date: 2018-05-29
陈雪玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] According to previous experiments, we speculate that macrophages may play an important role in immune escape, but what causes the changes in the expression of these factors in echinococcosis is still poorly understood
However, the relationship between PPARs and echinococcosis and its expression level have not been reported at home and abroad.

Method used

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  • Method for researching influence on macrophage polarization by PPARs
  • Method for researching influence on macrophage polarization by PPARs
  • Method for researching influence on macrophage polarization by PPARs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1: Changes of PPARs in the early stage of co-culture of RAW264.7 cells and Echinococcus granulosus and its regulation on macrophage polarization The expression levels of PPARs after RAW264.7 cells were infected with larvae, and the expression levels of M1 and M2 related factors in macrophages, so as to clarify the non-specific immune regulation of PPARs in the early stage of echinococcosis infection. It lays the foundation for further exploration of its role in Echinococcus evading the host's immune response.

[0100] 1 material

[0101] 1.1 Main instruments and reagents

[0102]

[0103]

[0104]

[0105] 1.2 Sources of main cells and pathogens collected

[0106] Cells: mouse macrophage cell line (RAW264.7) is preserved and stored in the laboratory.

[0107] Protozoan: the diseased liver of sheep infected with Echinococcus granulosus was obtained from the slaughterhouse in Changji City, Xinjiang.

[0108] 2 methods

[0109] 2.1 Preparation of prot...

Embodiment 2

[0180] Example 2: The expression level of PPARs in the early stage of co-culture of peritoneal macrophages and Echinococcus granulosus protoscoleum and its regulation on the polarization of macrophages

[0181] Objective: This study is to further study the role of primary cells on the basis of the previous cell lines, collect mouse peritoneal macrophages, and co-culture with Echinococcus to establish a model, and explore the PPARs after Echinococcus infection of mouse peritoneal macrophages and the expression levels of M1 and M2-related factors in macrophages, so as to clarify the non-specific immune regulation of PPARs in the early stage of echinococcosis infection. On this basis, PPARs inhibitors were used to down-regulate PPARs, and further elucidated its role in Echinococcus evading the host's immune response.

[0182] 1 material

[0183] 1.1 Materials and reagents

[0184] Main equipment name

production company

Protein Electrophoresis System

Ame...

Embodiment 3

[0280] Example 3: Early expression level of PPARs in mice infected with Echinococcus granulosus and its regulation on macrophage polarization

[0281] Objective: In this study, by establishing an animal model of BALB / c mice infected with echinococcosis, to further verify the expression level of PPARs in peritoneal macrophages of mice infected with echinococcosis, and the related factors of macrophage M1 and M2 types In order to elucidate the non-specific immunoregulatory effect of PPARs in the early stage of echinococcosis infection. And on this basis, PPARs inhibitors were used to down-regulate PPARs, and further elucidated its role in Echinococcus evading the host's immune response.

[0282] 1 material

[0283] 1.1 Materials and reagents (same as Example 2)

[0284] 1.2 Main pathogen collection and mouse source (same as embodiment 2)

[0285] 2 methods

[0286] 2.1 Collection, cultivation and activity identification of protoscoleiae (same as Example 1)

[0287] 2.2 Esta...

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Abstract

The invention discloses a method for researching the influence on macrophage polarization by PPARs. The method comprises the following steps: detecting the expression level of macrophage RRARs and regulation on macrophage polarization by establishing an echinococcus granulosus infected mouse model and an in-vitro protoscolex and macrophage co-culture system, inhibiting a PPARs passage on the basis, and further discussing the influence on macrophage polarization by the PPARs as well as the effect in the echinococcosis immune escape process.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a research method for the influence of PPARs on macrophage polarization. Background technique [0002] Hydatid disease, also known as echinococcosis, is a zoonotic chronic infectious disease produced by the larvae of Echinococcus granulosus parasitic in humans and animals, and is also an endemic infectious disease. High incidence in pastoral areas. There are two common types, Cystic echinococcosis (CE) and Alveolar echinococcosis (AE), which are infected by the larvae of Echinococcus granulosus and Echinococcus multilocularis, respectively. Cystic echinococcosis (Cysticechinococcosis, CE) caused by Echinococcus granulosus tapeworm is common in my country. This has major public health and economic implications. [0003] Echinococcosis is widely distributed globally and is common in Africa, South America, Eurasia, and northern China. In my country, it is mainly distributed in poorer and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876G01N33/68A61K49/00
CPCA61K49/0008C12Q1/6876C12Q2600/158G01N33/68G01N2333/70567
Inventor 陈雪玲吴向未陈聪哲
Owner 陈雪玲
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