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Composition with macrophage polarization targeted adjustment function and preparation method and application thereof

A technology of macrophages and compositions, applied in the field of medicine, can solve the problems of less attention to aseptic inflammatory response, narrow beneficiary population, and little curative effect, so as to increase active targeting, inhibit recurrence and metastasis, and reduce phagocytosis Effect

Inactive Publication Date: 2020-07-03
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are many immunotherapies on the market that can inhibit tumor progression after ablation therapy, but the beneficiary population is narrow and the effect is minimal. This type of immunotherapy combined with thermal ablation focuses more on the release of tumor-associated antigens and activation of the body caused by thermal ablation Anti-tumor immune response, but little attention has been paid to the aseptic inflammatory response of the body to thermal damage repair after thermal ablation, but related studies have shown that the tissue repair response after ablation therapy may promote tumor progression and recurrence

Method used

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  • Composition with macrophage polarization targeted adjustment function and preparation method and application thereof
  • Composition with macrophage polarization targeted adjustment function and preparation method and application thereof
  • Composition with macrophage polarization targeted adjustment function and preparation method and application thereof

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Effect test

Embodiment 1

[0027] This embodiment provides a method for preparing a composition capable of targeting and regulating the polarization of macrophages, including the following steps:

[0028] Preparation of NaOH / DEG precursor: Weigh 2g of NaOH with a balance and place it in a 50ml three-necked flask, inject 20ml of diethylene glycol into the flask, vacuumize at room temperature for 3min to discharge the air in the system, and heat the three-necked flask to 120°C under nitrogen protection , and reacted for 1h to obtain the NaOH / DEG precursor.

[0029] Preparation of water-soluble iron oxide nanoparticles (IONPs): Weigh 0.7g of polyacrylic acid and 0.54g of FeCl with a balance 3 ·6H 2 O was dissolved in 15mL of diethylene glycol, and the solution was vacuumed at 100°C for 30 minutes to discharge the air; heated to 220°C under nitrogen protection, and reacted with magnetic stirring for 30 minutes; extracted 2NaOH / DEG precursor, injected quickly, and continued to react for 10 minutes; the reac...

Embodiment 2

[0033] This embodiment provides a method for preparing a composition capable of targeting and regulating the polarization of macrophages, including the following steps:

[0034] Preparation of NaOH / DEG precursor: Weigh 0.5g NaOH with a balance and place it in a 50ml three-necked flask, inject 10ml of diethylene glycol into the flask, vacuumize at room temperature for 3-10min to discharge the air in the system, and heat the three-necked flask under nitrogen protection To 100°C, react for 0.5h to obtain the NaOH / DEG precursor.

[0035] Preparation of water-soluble iron oxide nanoparticles: weigh 0.05g of polyacrylic acid and 0.1g of FeCl with a balance 3 ·6H 2 O was dissolved in 10mL of diethylene glycol, and the solution was evacuated at 100°C for 30 minutes to discharge the air; heated to 180°C under nitrogen protection, and stirred by magnetic force for 30 minutes; extract 2ml of NaOH / DEG precursor, inject it quickly, and continue to react for 5-30 minutes; After the reacti...

Embodiment 3

[0038] This embodiment provides a method for preparing a composition capable of targeting and regulating the polarization of macrophages, including the following steps:

[0039] Preparation of NaOH / DEG precursor: Weigh 2.5g NaOH with a balance and place it in a 50ml three-necked flask, inject 20ml of diethylene glycol into the flask, vacuumize at room temperature for 10min to discharge the air in the system, and heat the three-necked flask to 120°C under nitrogen protection. °C, reacted for 2h to obtain the NaOH / DEG precursor.

[0040] Preparation of water-soluble iron oxide nanoparticles: weigh 1.5g of polyacrylic acid and 2g of FeCl with a balance 3 ·6H 2 O was dissolved in 20mL of diethylene glycol, and the solution was vacuumed at 100°C for 30 minutes to discharge the air; heated to 230°C under nitrogen protection, and reacted with magnetic stirring for 30 minutes; extracted 10ml of NaOH / DEG precursor, injected quickly, and continued to react for 30 minutes; the reaction ...

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Abstract

The present invention discloses a composition with a macrophage polarization targeted adjustment function and a preparation method and application thereof. The composition comprises a targeting carrier capable of targeting macrophages and nano iron oxide coating the interior of the targeting carrier. The targeting carrier coats the nano iron oxide to change the surface structure and performance ofthe nano iron oxide, so that the nano iron oxide can better avoid identification of the macrophages, phagocytosis of nanoparticles by the macrophages is reduced, and the active targeting property ofthe nano iron oxide to a tumor tissue is improved; and the nano iron oxide particles have the potential of polarizing the macrophages, and can change tumor microenvironment components, thereby inhibiting tumor recurrence and metastasis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a composition capable of targeting and regulating the polarization of macrophages, a preparation method and application thereof. Background technique [0002] At present, there are many immunotherapies on the market that can inhibit tumor progression after ablation therapy, but the beneficiary population is narrow and the effect is minimal. This type of immunotherapy combined with thermal ablation focuses more on the release of tumor-associated antigens and activation of the body caused by thermal ablation Anti-tumor immune response, but little attention has been paid to the aseptic inflammatory response of the body to thermal damage repair after thermal ablation, but related studies have shown that tissue repair response after ablation therapy may promote tumor progression and recurrence. Contents of the invention [0003] In order to solve the above-mentioned pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K33/26A61K9/51A61K47/10A61K47/69A61P35/00A61P35/04
CPCA61K9/5146A61K33/26A61K47/549A61K47/6929A61P35/00A61P35/04
Inventor 梁萍于杰崔瑞窦健萍孟宪伟张东云穆梦娟
Owner GENERAL HOSPITAL OF PLA
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