A living cell drug delivery system based on macrophages, its preparation method and application

A technology of macrophages and drug-carrying systems, which is applied in the direction of cell culture active agents, biochemical equipment and methods, and pharmaceutical formulations. The effect of maintaining stability and maintaining activity
CN110507826BActive Publication Date: 2021-10-01SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Publication Date
2021-10-01

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Abstract

The invention discloses a macrophage-based living cell drug-carrying system, a preparation method and application thereof. The macrophage-based live cell drug delivery system comprises macrophages, anchored chemotherapeutic drugs and anchored cytolytic groups. The preparation method includes the following steps: taking mouse bone marrow cells, culturing them in vitro, adding recombinant mouse macrophage colony stimulating factor and mouse breast cancer 4T1 cell culture supernatant or lipopolysaccharide LPS into the culture medium, and continuing to culture, Induce macrophage polarization to obtain regulatory M1 or M2 macrophages; co-incubate anchored chemotherapeutic drugs and anchored cytolytic groups in macrophage culture medium to prepare macrophage-based Live cell drug delivery system. The macrophage-based living cell drug-loading system prepared above can actively target the lung metastasis site of breast cancer, and effectively inhibit the lung metastasis of breast cancer.
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Description

technical field

[0001] The invention belongs to the field of pharmacy, and in particular relates to a living cell drug delivery system based on macrophages, its preparation method and application. Background technique

[0002] Breast cancer metastasis is the main cause of death in breast cancer patients. In the early stage of breast cancer, surgical resection can be used for treatment, but surgical resection is difficult to completely remove the lesion, and the prognosis is poor, and it is easy to relapse and metastasis. Chemotherapeutic drugs can inhibit the proliferation and spread of breast cancer cells, but due to poor selectivity of chemotherapeutic drugs, they usually have serious side effects, and after chemotherapy, it is easy to cause breast cancer tumor cells to develop drug resistance, which leads to the failure of clinical treatment. Radiation therapy can kill tumor cells, but the normal cells adjacent to the tumor will also be damaged to varying degrees, and it...

Claims

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