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Macrophage polarization regulator and application thereof in promoting platelet generation

A technology of macrophages and platelets, applied in the field of biomedicine

Active Publication Date: 2020-12-01
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report to promote the normalization of the microenvironment of bone marrow megakaryocytes by regulating the polarization of bone marrow macrophages, and ultimately improve the function of megakaryocytes and platelet production

Method used

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  • Macrophage polarization regulator and application thereof in promoting platelet generation
  • Macrophage polarization regulator and application thereof in promoting platelet generation
  • Macrophage polarization regulator and application thereof in promoting platelet generation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Detecting the proportions of each subpopulation of bone marrow mononuclear cells in PT patients, well implanted (GGF) patients and healthy donors (HD)

[0059] After hematopoietic stem cell transplantation, collect PT patients, GGF patients, and HD bone marrow whole blood with similar clinical characteristics except hemograms such as age, gender, and underlying diseases, and take 200 μl of them in a flow tube to label the surface markers of each subgroup of mononuclear cells and perform flow cytometry detection , a typical stream diagram is shown in figure 1 .A. Analysis of 6 cases of PT, GGF and HD by flow cytometry found that compared with GGF patients and HD patients, the proportion of bone marrow classical mononuclear cells in PT patients decreased ( figure 1 .B), the proportion of intermediate and atypical monocytes increased ( figure 1 .C,D).

Embodiment 2

[0060] Example 2: Detection of the proportion of polarized subpopulations of bone marrow macrophages in PT patients, well implanted (GGF) patients and healthy donors (HD)

[0061] Collect whole blood from PT patients, GGF patients and HD bone marrow with similar clinical characteristics after hematopoietic stem cell transplantation, except for hemograms such as age, gender, and basic diseases, and take 200 μl to mark the surface markers of macrophage polarized subsets in a flow tube and perform flow analysis. Type detection, flow type typical picture is as follows figure 2 .A. Polarization of bone marrow macrophages to M1 increased in PT patients ( figure 2 .B), the polarization of macrophages decreased to M2 ( figure 2 .C), presenting a state of macrophage polarization imbalance with significantly increased M1 / M2 ( figure 2 .D).

Embodiment 3

[0062] Example 3: Detection of phagocytosis and migration functions of bone marrow macrophages in PT patients and patients with good engraftment (GGF)

[0063] Bone marrow whole blood was collected from PT patients and GGF patients with similar clinical features after hematopoietic stem cell transplantation, such as age, gender, and underlying diseases, and bone marrow mononuclear cells were extracted and cultured into macrophages in vitro. First, in vitro phagocytosis and migration experiments were performed to evaluate whether there were basic functional differences between the two groups of macrophages. The experimental results showed that the migration function of PT macrophages and GGF macrophages ( image 3 .A,B) and phagocytosis ( image 3 .C,D) No significant difference.

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Abstract

The invention mainly relates to a macrophage polarization regulator and application thereof in promoting platelet generation. Specifically, the present invention relates to a pharmaceutical composition for treating thrombocytopenic disease in a subject, and the composition comprises the macrophage polarization regulator and a pharmaceutically acceptable carrier. The invention also relates to a kitfor diagnosing thrombocytopenic disease in the subject, and the kit comprises a detection reagent for detecting a thrombocytopenic disease marker and / or a detection reagent for detecting macrophage polarization level. The invention also relates to the macrophage polarization regulator, the detection reagent for detecting the marker and application of the detection reagent for detecting the macrophage polarization level in the thrombocytopenic disease. The invention has important clinical significance for hierarchical diagnosis of diseases and accurate targeted therapy of diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, specifically, the invention mainly relates to a macrophage polarization regulator and its application in promoting platelet production. [0002] technical background [0003] Thrombocytopenia, also called thrombocytopenia, is a disease characterized by an abnormal decrease in the number of platelets in the peripheral blood. Causes include insufficient platelet production, excessive platelet destruction, or abnormal platelet distribution. Among them, delayed platelet engraftment (PT) after transplantation is a common thrombocytopenic disease. [0004] Delayed platelet engraftment after transplantation is one of the serious complications of allogeneic hematopoietic stem cell transplantation, which means that on the premise of complete donor chimerism, white blood cells and hemoglobin have reached stable implantation +60 days after transplantation, while platelets continue to be low. at 20×10 ...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K38/20A61P7/00C12Q1/6883G01N33/68G01N33/573
CPCA61K38/2026A61K38/2086A61K45/06A61P7/00C12Q1/6883C12Q2600/158G01N33/573G01N33/6893G01N2333/4703G01N2333/475G01N2333/755G01N2333/78G01N2333/91215G01N2800/222A61K2300/00
Inventor 黄晓军孔圆赵红艳温琦
Owner PEOPLES HOSPITAL PEKING UNIV
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