The invention provides an electrospun membrane stent for promoting rotator cuff tendon bone healing and inhibiting muscle fat infiltration and a preparation method thereof. The preparation method of the electrospun membrane stent comprises the following steps of 1, ultrasonically dispersing MSNs in ethanol, adding triethoxysilane, performing stirring, performing centrifuging to collect nanoparticles, re-dispersing in sulfuric acid, heating to collect a final product, performing washing, and freeze-drying to obtain MSN-COOH nanoparticles; 2, adding lithium salt and the MSN-COOH into deionized water, performing stirring, then performing centrifuging, removing supernate, and drying precipitate in vacuum for 40-50 hours to obtain Li+@MSNs; and 3, dissolving a high polymer material and the Li+@MSNs in HFIP, stirring to prepare a uniform solution, and then preparing the electrospun membrane stent with a single fiber in a cowpea-like structure by adopting an electrospinning technology. The electrospun membrane stent can promote the bone healing of a rotator cuff tendon bone interface and inhibit rotator cuff muscle fat infiltration, meanwhile, provides an instant mechanical support effect after operation, and can provide a new idea for clinically repairing old rotator cuff injuries.