Biological patch and preparation method thereof

A biological patch, umbilical cord technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as poor effect, and achieve the effect of promoting cell differentiation and improving effect

Inactive Publication Date: 2021-07-27
THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the present application provides a biological patch and its preparation method, aiming to solve the problem of poor effect of extracellular matrix material regeneration and repair of rotator cuff injury

Method used

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  • Biological patch and preparation method thereof
  • Biological patch and preparation method thereof
  • Biological patch and preparation method thereof

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preparation example Construction

[0038] Therefore, the embodiment of the first aspect of the present application provides a method for preparing a biological patch, the method comprising:

[0039] S100, providing an isolated umbilical cord;

[0040] S200, pretreating the umbilical cord to remove umbilical cord blood and umbilical vessels contained in the umbilical cord;

[0041] S300, decellularizing the pretreated umbilical cord to make a decellularized umbilical cord;

[0042] S400, performing a cross-linking reaction on the decellularized umbilical cord and an inducer solution to obtain a composite scaffold, wherein the inducer solution is a solution capable of differentiating mesenchymal stem cells into chondrocytes;

[0043] S500, sequentially washing and freezing the composite scaffold to obtain a biological patch.

[0044] According to the preparation method of the embodiment of the present application, pretreatment, decellularization treatment, cross-linking reaction and washing, and freezing treatm...

Embodiment 1

[0078] The human umbilical cord was cut into about 2 cm long sections, and then washed with 0.9% sterile sodium heparin-physiological saline to remove blood stains in the human umbilical cord. The human umbilical cord was dissected longitudinally, two arteries and one vein in the human umbilical cord were stripped off, and the remaining tissue was flattened and trimmed neatly into sheets.

[0079] Rinse the trimmed umbilical cord three times with sterile water, 5 min each time. Umbilical cords were frozen at -80°C for 1 h and then thawed naturally. Repeat the steps of freezing and thawing 3 times.

[0080] Wash the umbilical cord three times with sterile water, 1 hour each time. Then put the umbilical cord into 0.1% trypsin / 0.1% EDTA mixed solution, shake at room temperature for 12 hours. Put into 1% Triton X-100 solution, shake at room temperature for 12 hours. Then shake and wash with sterile distilled water 3 times, 6 hours each time.

[0081] The KGN small molecule was ...

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Abstract

The invention discloses a biological patch and a preparation method thereof. The preparation method comprises the following steps: providing an in-vitro umbilical cord; pretreating the umbilical cord to remove umbilical cord blood and umbilical cord blood vessels contained in the umbilical cord; performing decellularization treatment on the pretreated umbilical cord to prepare a decellularized umbilical cord; performing a cross-linking reaction on the decellularized umbilical cord and an inducer solution, obtaining the composite scaffold, wherein the inducer solution is a solution capable of differentiating mesenchymal stem cells into cartilage cells; sequentially performing cleaning and freezing treatment on the composite scaffold, and obtaining the biological patch. The effect of repairing the rotator cuff injury is good.

Description

technical field [0001] The application belongs to the technical field of biological materials, and in particular relates to a biological patch and a preparation method thereof. Background technique [0002] Rotator cuff injury is the main cause of shoulder pain and dysfunction, and due to the unique structure of rotator cuff tendons and key bones, it is generally difficult to heal itself after rotator cuff injury. [0003] With the development of tissue engineering technology, more and more studies have found that biomaterials can promote regeneration of rotator cuff tendon and bone healing. Biomaterials, especially extracellular matrix materials, have good biocompatibility and can be used as carriers of seed cells and biological signal molecules, and play an important role in the regeneration and repair of rotator cuff injuries. However, the effect of regeneration and repair of extracellular matrix materials is poor, and the repair effect still needs to be improved. Cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3662A61L27/3687A61L27/3691A61L2430/10A61L2430/40
Inventor 郭全义苑志国高仓健曹福洋刘舒云睢祥黄敬香李浩杨振
Owner THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
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