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Stem-cell-like memorability T cell in-vitro inducer and method

A technology of stem cells and memory, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of lack of TSCM in vitro amplification methods, achieve important development value and promotion significance, good safety, and low cost Effect

Active Publication Date: 2017-02-15
SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of relatively simple and efficient methods for in vitro amplification of TSCM in the prior art

Method used

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  • Stem-cell-like memorability T cell in-vitro inducer and method

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Embodiment Construction

[0024] An in vitro expansion induction medium for stem cell-like memory T cells, the main body of which is a T cell culture medium, and a histamine H1 receptor antagonist is added to the culture medium.

[0025] As a further improvement of the above medium, the histamine H1 receptor antagonist is selected from the first generation histamine H1 receptor antagonist, the second generation histamine H1 receptor antagonist and the third generation histamine H1 receptor antagonist.

[0026] As a further improvement of the above medium, the histamine H1 receptor antagonist is selected from diphenhydramine, promethazine, chlorpheniramine, cyproheptadine, dechlorhydrazine, hydroxyzine, loratadine, cetidine Lizine, terfenadine, astemizole, abastine, fexofenadine, arbastine, mequinazine, mizolastine, ebastine, fexofenadine, desmethyl Astemizole, decarboxyloratadine. Further, the histamine H1 receptor antagonist is selected from diphenhydramine, promethazine, loratadine, and cetirizine. ...

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Abstract

The invention discloses a stem-cell-like memorability T cell in-vitro inducer and a method. The inventor finds through experiments that a CD8 initial T cell can be rapidly induced, differentiated and amplified into TSCM cells in vitro if a histamine H1 receptor antagonist is additionally added into a T cell induction medium, the ratio of the TSCM cells to the CD8 cell can be remarkably increased, and the absolute number of the TSCM cells can be remarkably increased. Properties and functions of the induced, differentiated and amplified TSCM cell are maintained. The security of the histamine H1 receptor antagonist to human bodies is already tested through clinical practice, and a long research and development cycle of a new drug can be avoided. Meanwhile, as a developed medicine, the histamine H1 receptor antagonist has the advantages of low cost and good security, the TSCM cells induced, differentiated and amplified with the histamine H1 receptor antagonist can be re-transfused without special treatment, a powerful practice basis can be provided for tumor cell treatment, and significant development values and popularization meanings can be achieved.

Description

technical field [0001] The invention relates to a cell culture inducer and a cell induction method, in particular to an in vitro inducer and method for stem cell-like memory T cells. Background technique [0002] Mature T cells are immune active and can recirculate through lymphatic vessels, peripheral blood and interstitial fluid to exert cellular immunity and immune regulation functions. The recycling of T cells is conducive to extensive exposure to antigenic substances entering the body, strengthening the immune response, and maintaining immune memory for a long time. It is expected to treat some specific diseases by inducing and expanding memory T cells in vitro and then infusing them back into the body. [0003] Among the induction and expansion methods of memory T cells, the more mature method is to use anti-CD3 and anti-CD28 antibodies to stimulate and induce differentiation. The stimulated differentiated T cells are obviously proliferated and activated, the original...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2500/30C12N2501/515C12N2501/51
Inventor 张辉张译文
Owner SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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