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Applications of interleukin-21 in preparation of stem cell-like memory T cell in vitro amplification inducer

A stem cell and interleukin technology, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of difficulty in obtaining sufficient amount, short survival time in vivo, and difficult long-term survival of cells, achieving high safety, The effect of good technical support

Inactive Publication Date: 2016-12-21
SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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Problems solved by technology

In recent years, more and more researchers have applied T cells to cell adoptive immunotherapy, but the adoptive reinfusion of T cells still faces some difficulties. For example, it is difficult for cells in the terminal stage to survive for a long time in vitro Obtain a sufficient amount of cells, the survival time in vivo is not long, etc.

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  • Applications of interleukin-21 in preparation of stem cell-like memory T cell in vitro amplification inducer
  • Applications of interleukin-21 in preparation of stem cell-like memory T cell in vitro amplification inducer
  • Applications of interleukin-21 in preparation of stem cell-like memory T cell in vitro amplification inducer

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Embodiment Construction

[0019] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. Human peripheral blood components were provided by Guangzhou Blood Center. Unless otherwise specified, the reagents, equipment and methods used in the present invention are conventional commercially available reagents, equipment and routinely used methods in the technical field.

[0020] Different concentrations of inducers on T SCM The effect of in vitro amplification

[0021] (1) Sorting out Naïve CD8+ T cells (CD8 + CD45RO - CD62L + ) in a 12-well plate, the expected number of cells per well is 1×10 6 Each, add T cell culture medium containing 1 μg / mL (final concentration) Anti-CD3 antibody and 1 μg / mL (final concentration) Anti-CD28 antibody;

[0022] (2) Followed by 5 different treatments, each group was added IL-21 with a final concentration of 0, 5, 10, 20, 40 ng / mL, and three replicate wells were set up for each treatmen...

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Abstract

The present invention discloses applications of interleukin-21 in preparation of a stem cell-like memory T cell in vitro amplification inducer. According to the present invention, with the addition of interleukin-21 as an inducer to a T cell induction culture medium, the differentiation amplification of Naive CD8+ T cells can be induced to obtain TSCM cells, the forming proportion and the absolute number of the TSCM cells are significantly increased, and the effect is significantly superior to the effect of the inducer IL-2 universally used in the cell adoptive immunotherapy; and the interleukin-21 is the natural cytokine secreted by human body, has the high safety, does not have the drug toxicity and other problems, can provide the good technical support for the extensive development of the adoptive immunotherapy, and has the important promotion significance.

Description

technical field [0001] The invention belongs to the technical field of in vitro expansion of T cells, and more specifically relates to an inducer and a method for in vitro expansion of stem cell-like memory T cells. Background technique [0002] Cell adoptive immunotherapy is a treatment method that activates and expands autologous or allogeneic immune cells in vitro and then infuses them into patients. It is considered to be the most potential immunotherapy at present. T cells play an important role in the human immune system, and their suppression and tolerance in the body will lead to a decline in immunity, thereby triggering the occurrence of infection and tumors. In recent years, more and more researchers have applied T cells to cell adoptive immunotherapy, but the adoptive reinfusion of T cells still faces some difficulties, for example, it is difficult for cells in the terminal stage to survive for a long time under in vitro culture conditions, and it is difficult to ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2501/2321C12N2501/51C12N2501/515
Inventor 张辉张译文陈颖诗
Owner SHENZHEN CITY OF REGENERATION BIOLOGICAL PHARMA TECH CO LTD
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