5 results about "Fluorescence-lifetime imaging microscopy" patented technology

A deep learning-based super-resolution fluorescence lifetime imaging microscopy (SR-FLIM) method includes the steps of: S1, performing fluorescence microscopic imaging on a sample to obtain confocal intensity images and stimulated emission depletion (STED) intensity images at a same location; S2, co-registering the acquired confocal and STED intensity images; S3, pairing the co-registered confocal and STED intensity images as input (Input) and ground truth (GT) to assemble a dataset; S4, partitioning the dataset into training and validation sets following a predefined ratio; and S5, constructing a network, and selecting hyperparameters and an optimizer. This method may achieve SR-FLIM within a conventional confocal FLIM system, surpassing spatial resolution limitations of FLIM, breaking through resolution barriers of conventional optical microscopy, while preserving normal fluorescence lifetime characteristics of fluorescent probes.

The present invention discloses a method for stimulated emission depletion (SEP) fluorescence lifetime imaging with super-resolution, which is applied in the field of optical microscope imaging technology. The method proposed in the present invention combines the principles of stimulated emission depletion and SEP fluorescence lifetime imaging, uses a loss laser pulse frequency that is half the excitation light pulse frequency, and adjusts the time interval between two pulse sequences such that the loss laser pulse is located after the adjacent excitation light pulse.By analyzing and processing the collected fluorescence lifetime data, a super-resolution fluorescence lifetime image is obtained. This solves the problem of the significantly reduced fluorescence lifetime of a stimulated emission depletion (SED) fluorescence lifetime imaging microscope under the influence of a high-energy loss laser, thus accurately reproducing the actual information of the fluorescent dye and the microenvironment. This innovative technology will provide important technical support for life science research, significantly improving the accuracy and effectiveness of research and promoting further development in the biomedical field.

Methods and systems to assess the viability of human oocytes for egg vitrification or cryopreservation process are disclosed herein. The method utilizes fluorescence lifetime imaging microscopy (FLIM) coupled with integrating an oocyte's morphological endpoints, such as shape and size, to assess and identify the viability in human oocytes. The method and system also use data analysis software capable of correlating PH values, FLIM data, and chromosomal health assessments to determine the viability of human oocytes.

The invention relates to the technical field of biological materials, in particular to a fluorescent probe for targeting mitochondrial G4DNA, a preparation method and fluorescence lifetime imaging application. The fluorescent probe for targeting mitochondrial G4DNA has membrane permeability, high light stability, mitochondrial targeting and selective recognition capability on mtDNA, especially mitochondrial G4 quadruplex (mtG4DNA), and can realize real-time analysis of mtDNA abundance, conformation and mtG4DNA dynamic change under the condition of no need of gene manipulation, fixation or amplification. Through combination of stimulated radiation depletion (STED) or structured illumination super-resolution imaging (SIM) and a fluorescence lifetime imaging microscope (FLIM), the probe can provide intensity and lifetime dual signals at the same time, accurately maps mtDNA aggregate remodeling, oxidative damage and three-dimensional spatial-temporal dynamics participated by a G4 structure, and can be used for detecting the fluorescence lifetime of the mtDNA aggregate. A universal and non-invasive imaging platform is provided for researching related mechanisms of mitochondrial dysfunction, senescence and neurodegenerative diseases (such as ALS).

The present invention relates to a method for determining the degree of oxidation of a polysaccharide using the ELIM (Fluorescence Lifetime Imaging Microscopy) technique.