8 results about "Fluorescence-lifetime imaging microscopy" patented technology

A deep learning-based super-resolution fluorescence lifetime imaging microscopy (SR-FLIM) method includes the steps of: S1, performing fluorescence microscopic imaging on a sample to obtain confocal intensity images and stimulated emission depletion (STED) intensity images at a same location; S2, co-registering the acquired confocal and STED intensity images; S3, pairing the co-registered confocal and STED intensity images as input (Input) and ground truth (GT) to assemble a dataset; S4, partitioning the dataset into training and validation sets following a predefined ratio; and S5, constructing a network, and selecting hyperparameters and an optimizer. This method may achieve SR-FLIM within a conventional confocal FLIM system, surpassing spatial resolution limitations of FLIM, breaking through resolution barriers of conventional optical microscopy, while preserving normal fluorescence lifetime characteristics of fluorescent probes.

The present invention discloses a method for stimulated emission depletion (SEP) fluorescence lifetime imaging with super-resolution, which is applied in the field of optical microscope imaging technology. The method proposed in the present invention combines the principles of stimulated emission depletion and SEP fluorescence lifetime imaging, uses a loss laser pulse frequency that is half the excitation light pulse frequency, and adjusts the time interval between two pulse sequences such that the loss laser pulse is located after the adjacent excitation light pulse.By analyzing and processing the collected fluorescence lifetime data, a super-resolution fluorescence lifetime image is obtained. This solves the problem of the significantly reduced fluorescence lifetime of a stimulated emission depletion (SED) fluorescence lifetime imaging microscope under the influence of a high-energy loss laser, thus accurately reproducing the actual information of the fluorescent dye and the microenvironment. This innovative technology will provide important technical support for life science research, significantly improving the accuracy and effectiveness of research and promoting further development in the biomedical field.

Methods and systems to assess the viability of human oocytes for egg vitrification or cryopreservation process are disclosed herein. The method utilizes fluorescence lifetime imaging microscopy (FLIM) coupled with integrating an oocyte's morphological endpoints, such as shape and size, to assess and identify the viability in human oocytes. The method and system also use data analysis software capable of correlating PH values, FLIM data, and chromosomal health assessments to determine the viability of human oocytes.

The application discloses a method for identifying cell apoptosis and necrosis and application thereof, and relates to the technical field of biological medicines. Through a two-photon microscope, fluorescence spectrum and a fluorescence lifetime imaging microscope, all cells in apoptosis and necrosis show an increase in red fluorescence intensity after excitation at 1060 nm, compared with living cells, the cells in apoptosis show a longer lipofuscin fluorescence lifetime in lysosomes, and the cells in necrosis show a shorter lipofuscin fluorescence lifetime. Based on the comparison of the fluorescence lifetime, the state change of the cells after drug treatment can be revealed, the technology can be applied to drug screening, and can have an influence on various fields of biomedical research and application, for example, drawing the heterogeneity of treatment response in a tumor microenvironment, marking single-cell drug resistance in spatial transcriptomics, and capturing the in-vivo dynamics of cell death in embryonic development.

The invention relates to the technical field of biological materials, in particular to a fluorescent probe for targeting mitochondrial G4DNA, a preparation method and fluorescence lifetime imaging application. The fluorescent probe for targeting mitochondrial G4DNA has membrane permeability, high light stability, mitochondrial targeting and selective recognition capability on mtDNA, especially mitochondrial G4 quadruplex (mtG4DNA), and can realize real-time analysis of mtDNA abundance, conformation and mtG4DNA dynamic change under the condition of no need of gene manipulation, fixation or amplification. Through combination of stimulated radiation depletion (STED) or structured illumination super-resolution imaging (SIM) and a fluorescence lifetime imaging microscope (FLIM), the probe can provide intensity and lifetime dual signals at the same time, accurately maps mtDNA aggregate remodeling, oxidative damage and three-dimensional spatial-temporal dynamics participated by a G4 structure, and can be used for detecting the fluorescence lifetime of the mtDNA aggregate. A universal and non-invasive imaging platform is provided for researching related mechanisms of mitochondrial dysfunction, senescence and neurodegenerative diseases (such as ALS).

The present invention relates to a method for determining the degree of oxidation of a polysaccharide using the ELIM (Fluorescence Lifetime Imaging Microscopy) technique.

A microscopy imaging system comprises a fluorescence lifetime imaging microscopy (FLIM) system comprising a pulsed light source configured to direct a plurality of excitation light pulses onto a sample, a photo detector configured to detect emitted fluorescent photons created by the plurality of excitation pulses interacting with the sample, and a FLIM data acquisition system configured to measure the time interval between the excitation light pulses and the detected emitted fluorescent photons, a scanning light microscopy (SLM) system comprising a SLM data acquisition system, a fast scanning mirror and a slow scanning mirror, wherein the mirrors are configured to scan the light pulses across the sample; and a data processing system communicatively connected to the FLIM and SLM systems. Microscopy imaging methods are also disclosed.

A method is provided comprising: positioning a slide that includes a concave region that is formed in a top surface of the slide and that can contain an object, such that a focal point of an objective lens of a fluorescence lifetime imaging microscopy (FLIM) device is within an area of the concave region between a bottom surface of the concave region and the top surface of the slide; and using the FLIM device to capture a sequence of wide field images of a portion of the object within a field of view of the objective lens.