47 results about "Single molecule localization" patented technology

A lens assembly, for use in microscopy imaging of a sample in a cryogenic environment, is described. A solid immersion lens which has a planar surface for accepting a sample for imaging is mounted within an aperture provided through the plane of a planar mount. The microscopy imaging may include using super-resolution optical imaging techniques such as single molecule localisation techniques.

The invention provides an acid-resistant light-controlled fluorescent molecular switch and its synthesis method and application. The specific molecular structure of the molecular switch uses rhodamine spiramide substituted with 3-primary amine or secondary amine as the basic structural unit, and its structural formula is as (1 ) shown. The acid-resistant light-controlled fluorescent molecular switch of the invention is applied in super-resolution fluorescent imaging, molecular probes, fluorescent sensing and other fields. The 3-primary amine or secondary amine substituted rhodamine spiroamide of the present invention not only has the performance of acid resistance, but also retains the performance of light-controlled molecular switch. Therefore, this kind of acid-resistant light-controlled fluorescent molecular switch can be applied in super-resolution imaging technology based on single-molecule localization, and it is not disturbed by the pH in the biological environment. In addition, the acid-resistant light-controlled fluorescent molecular switch of the present invention can also be used as a molecular fluorescent probe in the field of sensing and detection.

Systems and methods assessing the accuracy of at least one localization from a single molecule localization microscopy (SMLM) dataset containing a plurality of localizations are described. The systems and methods calculate a Wasserstein-induced flux (WIF) value indicative of a confidence in the accuracy of a localization within the SMLM data set.

The invention discloses a super-resolution optical imaging probe for living cells and a preparation method of the super-resolution optical imaging probe. Quantum dots are taken as a fluorophore, cell membrane penetrating peptides and nucleic acid aptamers are coupled on the surfaces of the quantum dots, the cell membrane penetrating peptides are used for promoting the probe to penetrate through the living cells, the nucleic acid aptamers are used for specific binding with nuclear membrane receptor protein of the living cells, and cell nucleus positioning of the probe is realized. The quantum dots with scintillating effects are taken as the fluorophore and are adapted to super-resolution optical imaging based on a single-molecule positioning method by means of the high luminous intensity and the fluorescence scintillation characteristic; meanwhile, the quantum dots with scintillating effects are taken as the fluorophore, a special imaging buffering solution is not required, therefore, the probe is adapted to super-resolution imaging of the living cells; besides, the preparation method is simple and convenient to operate, the cost is low, and the time cost and the economic cost are effectively saved.

The invention discloses a single-molecule positioning micro-imaging method, an optical assembly and an imaging system. The method comprises the following steps: establishing an optical module with a double helix point dispersion function and a deformed multi-value pure phase grating multi-order imaging property; after fluorescence beams emitted by to-be-tested molecules of multiple sample surfacesare emitted through the optical module, carrying out imaging at different positions of the same detection surface, so as to respectively generate double helix images; determining transverse positionsof the to-be-tested molecules according to positions of the centers of double helix sidelobes in the double helix images on the imaging surfaces; and determining the axial positions of the to-be-tested molecules according to the middle points of the double helix sidelobes in the double helix images and a rotation angle of a connection line between the two sidelobes. The molecular information of multiple layers in the sample can be imaged at different positions of the same detection surface in a double helix manner, so that the axial positioning range and resolution ratio of a double helix point spread function can be improved without scanning, and the problem of large field depth detection in single molecule positioning and tracing techniques in living cells is solved.