Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

BSA-based super-resolution imaging probe as well as preparation method and application thereof

A super-resolution imaging and probe technology, applied in the field of super-resolution imaging probes, can solve the problems of complex imaging process, troublesome, and limited super-resolution imaging applications, and achieve the effect of long blinking time and good photostability

Active Publication Date: 2018-11-23
SOUTHEAST UNIV
View PDF15 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These requirements make the PALM / STORM imaging process relatively complicated and cumbersome, and further limit the application of super-resolution imaging in live cell imaging.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • BSA-based super-resolution imaging probe as well as preparation method and application thereof
  • BSA-based super-resolution imaging probe as well as preparation method and application thereof
  • BSA-based super-resolution imaging probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Dissolve 2 mg of BSA in 200 μL of PBS solution, adjust the pH to 8.3 with 0.5 M sodium bicarbonate, then add 2 μL of 1 mg / mL Alexa Fluor 594 NHS ester to the solution and react on a shaking table at room temperature for 1 hour to obtain a solution It is the BSA solution coupled with Alexa Fluor 594.

[0039]Dissolve 20 mg of BSA in 2 mL of PBS solution, and then add 200 μL of the BSA solution coupled with AlexaFluor 594 prepared above to obtain a mixed BSA solution (total concentration of BSA is 10 mg / mL). Stir and continue to add 2 mL of absolute ethanol, then add 50 μL of 8% (v / v) glutaraldehyde solution, and stir for 12 h (final concentration of glutaraldehyde is 0.18%) to obtain dye-incorporated Finally, the sample was purified 5 times with a 50 KD ultrafiltration tube at 6500 round / min for 20 min to obtain BSA nanospheres doped with Alexa Fluor 594. Subsequently, take 500 μL of BSA nanosphere solution mixed with Alexa Fluor594, add 50 μL of 10% (v / v) glutaraldehyd...

Embodiment 2

[0042] Dissolve 5 mg of BSA in 200 μL PBS solution, adjust the pH to 8.3 with 0.5 M sodium bicarbonate, then add 5 μL of 1 mg / mL Alexa Fluor 594-NHS easter to the solution and react in a shaker at room temperature for 1 hour, and the obtained solution is BSA solution conjugated with Alexa Fluor 594.

[0043] Dissolve 20 mg of BSA in 2 mL of PBS solution, and then add 200 μL of the BSA solution coupled with AlexaFluor 594 prepared above to obtain a mixed BSA solution (total BSA concentration 11.4 mg / mL). Stir and continue to add 2 mL of absolute ethanol, then add 50 μL of 8% (v / v) glutaraldehyde solution, and stir for 18 hours (the final concentration of glutaraldehyde is 0.18%) to obtain the dye-incorporated BSA nanosphere solution, and finally the sample was purified 5 times with a 50KD ultrafiltration tube at 6500 round / min for 20 minutes to obtain BSA nanospheres mixed with Alexa Fluor 594. Then, take 500 μL of BSA nanosphere solution mixed with AlexaFluor 594, add 50 μL o...

Embodiment 3

[0045] Dissolve 2 mg of BSA in 200 μL of PBS solution, adjust the pH to 8.5 with 0.5 M sodium bicarbonate, then add 2 μL of 1 mg / mL Alexa Fluor 594-NHS easter to the solution and react in a shaker at room temperature for 1 hour, and the obtained solution is A solution in BSA conjugated with Alexa Fluor 594.

[0046] Dissolve 20 mg of BSA in 2 mL of PBS solution, and then add 200 μL of the BSA solution coupled with AlexaFluor 594 prepared above to obtain a mixed BSA solution (total concentration of BSA is 10 mg / mL). Stir and continue to add 4 mL of absolute ethanol, then add 50 μL of 8% (v / v) glutaraldehyde solution, and stir for 16 hours (the final concentration of glutaraldehyde is 0.18%) to obtain BSA nanoparticles mixed with dye. sphere solution, and finally the sample was purified 5 times with a 50KD ultrafiltration tube at 6500 round / min for 20 minutes to obtain BSA nanospheres doped with Alexa Fluor 594. Subsequently, take 500 μL of the BSA nanosphere solution mixed wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a BSA-based super-resolution imaging probe as well as a preparation method and application thereof. The super-resolution imaging probe is BSA nanometer balls coated with AlexaFluor 594 dye; in addition, HER2 antibodies are coupled on the surface. The probe has the fluorescence flickering features and can be used for super-resolution unimolecule imaging. In addition, the probe can be used for targeting high-EHR2-expression tumor cells and can enter the cells through receptor-mediated endocytosis; through an SMLM imaging technology, the tumor cells after the probe intakecan be subjected to super resolution optical imaging. The application of the super-resolution imaging probe in the cells to super-resolution imaging can be realized; an SMLM microscope is used; the distribution of the probe in the cells can be precisely positioned.

Description

technical field [0001] The invention relates to the field of super-resolution imaging probes, in particular to a BSA-based super-resolution imaging probe and its preparation method and application. Background technique [0002] The super-resolution microscopy technology that has emerged in recent years has the ability to image subcellular structures beyond the optical diffraction limit, which has revolutionized our research on cells, because super-resolution microscopy can use patterned illumination, Such as stimulated emission depletion (STED), or photo-activated localization microscopy (PALM) and optical reconstruction microscopy (STORM) through image reconstruction. At the same time, thanks to the continuous development of currently available fluorescent probes, the ultimate spatial resolution of PALM / STORM via single-molecule localization can almost achieve molecular tracking in cells. Conceptually, PALM / STORM achieves random switching of fluorophores between fluorescen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 宗慎飞潘凤梅王著元崔一平
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com