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Anti-bleaching unimolecule positioning three-dimensional super-resolution microscopy system

A microscopic system and super-resolution technology, applied in the field of optical instruments, can solve the problem of fluorescent protein molecules being bleached, so as to avoid bleaching and improve work efficiency

Inactive Publication Date: 2018-04-20
CHINA JILIANG UNIV
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Problems solved by technology

[0004] The present invention aims at the problem that fluorescent protein molecules are bleached when scanning in the depth direction in the previous three-dimensional SMS fluorescence microscopy technology, and proposes a new anti-bleaching single-molecule localization three-dimensional super-resolution microscopy system

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  • Anti-bleaching unimolecule positioning three-dimensional super-resolution microscopy system

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Embodiment Construction

[0035] The present invention will be described below in conjunction with the accompanying drawings, but the present invention is not limited thereto.

[0036] like figure 1 Shown is an optical path diagram of an embodiment of an anti-bleaching single-molecule localization three-dimensional super-resolution microscopy system of the present invention. The super-resolution microscopy system of this embodiment includes:

[0037] 1. First laser; 2. First lens; 3. Second lens; 4. Phase grating; 5. Third lens; 6. First dichroic mirror; 7. Objective lens; 8. Sample stage; 9. Filter 10, the fourth lens; 11, the detector; 12, the second dichromatic mirror; 13, the second laser;

[0038] Wherein, the first laser 1 is preferably Mai Tai femtosecond laser produced by Spectra-Physics Company. The first laser 1 emits a waveband from 825nm to 835nm, a peak wavelength of 830nm, a pulse width of less than 100 femtoseconds, and a power of 1.5 watts. After passing through the first lens 2 and ...

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Abstract

The invention discloses an anti-bleaching unimolecule positioning three-dimensional super-resolution microscopy system. The anti-bleaching unimolecule positioning three-dimensional super-resolution microscopy system comprises a first laser, a beam expanding and collimating unit, a phase grating, a third lens, a first dichroic mirror, an objective lens, a sample table, an optical filter, a fourth lens, a detector, a second dichroic mirror and a second laser. The anti-bleaching unimolecule positioning three-dimensional super-resolution microscopy system utilizes a time domain focusing characteristic, fluorescence excitation of a sample is generated only on a time domain focusing plane, and meanwhile, fluorescence excitation in the whole time domain focusing plane can be achieved. Therefore,compared with the prior art, bleaching of a sample in a non-focusing plane can be avoided, and the working efficiency of SMS fluorescence microscopy technology can be effectively improved.

Description

technical field [0001] The invention relates to the fields of optical instruments and biomedical microscopic imaging, in particular to an anti-bleaching single-molecule localization three-dimensional super-resolution microscopy system and method. Background technique [0002] The current research in the field of biomedicine requires higher and higher resolution of microscopic systems. Researchers need to understand the three-dimensional structure of various microscopic substances. However, the spot size of traditional white-light wide-field microscopes and laser confocal microscopes cannot achieve this. resolution, and the emergence of super-resolution microscopy system perfectly solves this problem. In super-resolution microscopy, current research hotspots mostly focus on various types of fluorescence microscopy, such as stimulated emission depletion (STED) fluorescence microscopy and single-molecule localization fluorescence microscopy (SMS). Compared with STED fluorescen...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6458G01N21/6486
Inventor 李旸晖周辉李雨雪刘小煜夏成樑
Owner CHINA JILIANG UNIV
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