Real-time single-molecule positioning method guaranteeing precision and system thereof

A positioning method and single-molecule technology, applied in image analysis, 2D image generation, instruments, etc., can solve the problem of increasing the difficulty of super-resolution image positioning speed, and achieve the effect of speeding up the technical effect.

Active Publication Date: 2016-01-13
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this also brings another challenge—since the detection speed of full-rate sCMOS is about 47 times that of EMCCD, the amount of image data that can be collected by sCMOS in one second is more, which is to improve Speed ​​of localization of super-resolved images adds to the difficulty

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  • Real-time single-molecule positioning method guaranteeing precision and system thereof
  • Real-time single-molecule positioning method guaranteeing precision and system thereof
  • Real-time single-molecule positioning method guaranteeing precision and system thereof

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Embodiment 1

[0075] see figure 1 , the real-time single-molecule localization method with guaranteed accuracy provided by the embodiment of the present invention includes:

[0076] Step S110: Perform region extraction on the image to be processed based on pixel-level positioning parameters;

[0077] To illustrate this step, step S110 specifically includes:

[0078] Obtain the pixel value of each pixel in the image to be processed;

[0079] Judging whether the obtained pixel value is greater than a preset threshold;

[0080] If so, obtain the coordinates of the pixel point corresponding to the maximum pixel value B, and the coordinates of the pixel point corresponding to the maximum pixel value B are pixel-level positioning parameters;

[0081] Extracting an image of a certain area centered on the coordinates of the pixel point corresponding to the obtained maximum pixel value B;

[0082] Wherein, a certain area is determined by the image to be processed.

[0083] In order to improve t...

Embodiment 2

[0117] see figure 2 , the real-time single-molecule localization system with guaranteed accuracy provided by the embodiment of the present invention includes:

[0118] A region extraction module 100, configured to perform region extraction on the image to be processed based on pixel-level positioning parameters;

[0119] In this embodiment, the region extraction module 100 specifically includes:

[0120] A pixel value acquisition unit, configured to acquire the pixel value of each pixel in the image to be processed;

[0121] a judging unit, configured to judge whether the acquired pixel value is greater than a preset threshold;

[0122] A pixel point acquisition unit, used to obtain the coordinates of the pixel point corresponding to the maximum pixel value B if the judgment result of the judgment unit is yes, and the coordinates of the pixel point corresponding to the maximum pixel value B are pixel-level positioning parameters;

[0123] The area extraction execution unit...

Embodiment 3

[0154] The specific steps of positioning the super-resolution image through the system provided in the second embodiment above include:

[0155] see image 3 First, the original image data is detected by the detector, and the obtained original image data is transmitted to the computer side through the two-point CameraLink sub-card for traditional control and data transmission, and the other way transmits the image data to the FPGA On the board, and cache in the DRAM buffer area on the FPGA board. Then the image data in the DRAM buffer area is read out to the line buffer area to obtain 3x3 image pixels to be processed. The denoising module uses a 3x3 mean value template to convolve with the obtained 3x3 image pixels to be processed to obtain a filtered image. The denoising module transmits the filtered image to the area extraction module 100 to judge the fluorescent points. The judging method is to obtain the standard deviation of the gray value of a series of filtered image...

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Abstract

The invention discloses a real-time single-molecule positioning method guaranteeing precision and a system thereof. The method comprises the steps that region extraction is performed on an image to be processed based on pixel-level positioning parameters firstly; then coarse positioning is performed on the extracted image so that the subpixel-level positioning parameters of the extracted image are obtained; and then fine positioning is performed on the extracted image based on the subpixel-level positioning parameters of the extracted image. The initial value (the subpixel-level positioning parameters) obtained through coarse positioning is closer to a real value, based on which fine positioning calculation is performed so that the number of times of iteration in fine positioning is reduced, and thus positioning speed of a super-resolution image is accelerated. Therefore, positioning speed of the super-resolution image is accelerated under the condition of guaranteeing positioning precision.

Description

technical field [0001] The invention relates to the technical field of super-resolution positioning imaging, in particular to a method and system for real-time single-molecule positioning with guaranteed accuracy. Background technique [0002] In the field of fluorescence microscopy, super-resolution microscopy imaging technology has broken through the diffraction limit and reached a resolution of tens of nanometers, thus providing biologists with an effective method for studying tiny structures and has been widely used. PhotoActivated Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM) are a type of super-resolution microscopy imaging technology. They are based on the optical imaging system of total internal reflection and software algorithms to realize Nanoscale resolution of super-resolution imaging, and are collectively referred to as super-resolution localization imaging techniques. [0003] Super-resolution positioning imaging techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T11/00G06T7/00
Inventor 黄振立李梦婷李路长
Owner HUAZHONG UNIV OF SCI & TECH
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