The invention discloses a
fluorescence in-situ hybridization
image contour enhancement method based on illumination fusion and local binary, relates to the field of
image processing, and solves the problems of different dye concentrations and the like due to image defects such as non-uniform
local illumination and relatively large
noise in an acquired image during existing
tissue cell fluorescence image preprocessing. The method comprises the following steps: removing
noise in an image by adopting a median filtering method, calculating a linear equation to obtain a texture weight of the image, performing analysis
processing by utilizing the texture weight and a
smoothing function to obtain a brightness image and a smooth image, and performing gray
processing to obtain gray distribution information; adopting a local binary
algorithm to select a coefficient, and performing enhancement
processing on the image to obtain an illumination enhanced image; the contour feature image and the illumination enhancement image are subjected to weighted addition, eight neighborhoods around eight
maximum pixel values are analyzed, an area with a
high energy gradient is recognized through a piecewise linear function and a thermodynamic diagram, and a high-frequency
signal area is the contour area of the
fluorescence in-situ hybridization
tissue cell image.