Super-resolution microscopic scale based on quantum dot and DNA origami nano co-assembly structure

Abstract

The invention discloses a super-resolution microscopic scale based on a quantum dot and DNA origami nano co-assembly structure, and belongs to the technical field of nano materials. The super-resolution microscopic scale is used for demarcating and calibrating the resolution of an ultrahigh-resolution fluorescence microscope system. According to the super-resolution microscopic scale based on thequantum dot and DNA origami nano co-assembly structure, quantum dots (2) and DNA single strands (3) are coupled and then modified to the fixed site of a DNA origami (1) (a triangle is taken as an example) to form the nano-scale with a fixed distance, so that the problem that the super-resolution microscopy lacks a high-precision scale to verify the accuracy of an obtained super-resolution image issolved. Through the super-resolution technology to break through the diffraction limit of light, the accuracy of the obtained super-resolution image is verified according to the fixed distance (20-200 nm) between the quantum dots on the same DNA origami structure. The super-resolution microscopic scale based on the quantum dot and DNA origami nano co-assembly structure is used for representing the imaging resolution of a positioning super-resolution monomolecular positioning microimaging technology (SMLM), and has the advantages of high brightness, stability, reusability and easiness in making different sizes.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a super-resolution microscale based on a quantum dot and DNA origami nano co-assembly structure. Background technique [0002] With the development and needs of modern science and technology, fluorescence microscopy imaging has become an important imaging method for modern life science research. People can use it to locate organelles and realize the research of dynamic information of cell function and interaction process. However, due to the limitation of the optical diffraction limit, finer subcellular structures cannot be observed. The ultra-high-resolution fluorescence microscope emerging in recent years has broken through the diffraction limit of light and can achieve a resolution of 20nm to 200nm, which has higher resolution and higher positioning accuracy than traditional optical microscopes. Among them, SMLM (Single Molecule Localization Microscopy), bas...

AI Technical Summary

Problems solved by technology

[0006] The first purpose of the present invention is to provide a super-resolution microscale based on quantum dots and DNA origami nano co-assembly structure, to solve the problem of existing super-resolution microscopy technology (SMLM (SingleMolecule Localization Microscopy) ) as an example), the technical problems of ultra-high resolution system testing and calibration in) are used for the calibration of ultra-high resolution fluorescence microscope system resolution, and a unified scale is provided; the super-resolution microscopic scale of the present invention has high brightness and stable structure , easy to customize different sizes, reusable

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