Early cancer diagnosis device based on combination of auto-fluorescence lifetime imaging and fluorescence spectroscopy
A technology of autofluorescence and fluorescence spectroscopy, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of inability to reflect the differences in the microenvironment of the source, and achieve the effects of fast detection speed, convenient operation, and clear principle
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Embodiment 1
[0047] according to figure 1 with figure 2 As shown, a measurement device combining autofluorescence lifetime imaging and fluorescence spectrum of the present invention was made. First, the measurement of the autofluorescence lifetime imaging of the biological sample to be tested is carried out: the laser 1 emits picosecond pulsed laser or femtosecond pulsed laser; the laser emitted by the laser passes through two vibrating mirrors 2 in the XY direction to scan the XY plane; the excitation light passes through The reflection of the first reflection mirror 3 and the second reflection mirror 4 is adjusted to the dichroic mirror 5; the dichroic mirror 5 fully reflects the excitation light; the reflected excitation light enters the objective lens 7 through the convergence of the first converging lens group 6; the objective lens 7 will The converged light is focused on the tested biological sample; the autofluorescence emitted by the tested biological sample passes through the ob...
Embodiment 2
[0050] Implementation according to Example 1: First, the measurement of fluorescence lifetime imaging is performed on the epithelial region of human cervical tissue. Place the examined cervical tissue slice on the stage 8, and use a 60 times objective lens to observe the morphology of the epidermal cells of the cervical tissue. Advance the movable slider 11 to position 25, use picosecond pulsed laser light with a wavelength of 50 MHz and 405 nm to excite the fluorescent substance of the examined cervical tissue, the first color filter 13 and the second color filter in the fluorescence collection light path 17 are cut-off filters of 430 nm high pass. After the signal amplified by the photomultiplier tube 14 is processed by the host computer 20 equipped with a time-correlated single photon counter, the fluorescence lifetime is fitted by the fluorescence lifetime processing software and the result of obtaining the fluorescence lifetime image is as follows: image 3 shown.
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