A Device Combining Autofluorescence Lifetime Imaging and Fluorescence Spectroscopy for Early Diagnosis of Cancer

A technology of autofluorescence and fluorescence spectroscopy, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of inability to reflect the differences in the source microenvironment, and achieve the effects of fast detection speed, wide application prospects and simple equipment.

Active Publication Date: 2017-12-01
FUDAN UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Fluorescence spectrum can provide very important information, but it is only an averaged result, which cannot reflect the source of different fluorescent molecular substances in biological tissues or the microenvironmental differences of the same fluorescent molecule

Method used

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  • A Device Combining Autofluorescence Lifetime Imaging and Fluorescence Spectroscopy for Early Diagnosis of Cancer
  • A Device Combining Autofluorescence Lifetime Imaging and Fluorescence Spectroscopy for Early Diagnosis of Cancer
  • A Device Combining Autofluorescence Lifetime Imaging and Fluorescence Spectroscopy for Early Diagnosis of Cancer

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Embodiment 1

[0047] according to figure 1 and figure 2 As shown, a measurement device combining autofluorescence lifetime imaging and fluorescence spectrum of the present invention was made. First, the measurement of the autofluorescence lifetime imaging of the biological sample to be tested is carried out: the laser 1 emits picosecond pulsed laser or femtosecond pulsed laser; the laser emitted by the laser passes through two vibrating mirrors 2 in the XY direction to scan the XY plane; the excitation light passes through The reflection of the first reflection mirror 3 and the second reflection mirror 4 is adjusted to the dichroic mirror 5; the dichroic mirror 5 fully reflects the excitation light; the reflected excitation light enters the objective lens 7 through the convergence of the first converging lens group 6; the objective lens 7 will The converged light is focused on the tested biological sample; the autofluorescence emitted by the tested biological sample passes through the obj...

Embodiment 2

[0050] Implementation according to Example 1: First, the measurement of fluorescence lifetime imaging is performed on the epithelial region of human cervical tissue. Place the examined cervical tissue slice on the stage 8, and use a 60 times objective lens to observe the morphology of the epidermal cells of the cervical tissue. Advance the movable slider 11 to position 25, use picosecond pulsed laser light with a wavelength of 50 MHz and 405 nm to excite the fluorescent substance of the examined cervical tissue, the first color filter 13 and the second color filter in the fluorescence collection light path 17 are cut-off filters of 430 nm high pass. After the signal amplified by the photomultiplier tube 14 is processed by the host computer 20 equipped with a time-correlated single photon counter, the fluorescence lifetime is fitted by the fluorescence lifetime processing software and the result of obtaining the fluorescence lifetime image is as follows: image 3 shown.

[00...

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Abstract

The invention belongs to the technical field of medical equipment, and in particular relates to a device for early diagnosis of cancer in combination with autofluorescence lifetime imaging and fluorescence spectrum. The invention couples fluorescence lifetime imaging measurement and fluorescence spectrum measurement with a laser scanning confocal microscope to observe the cytological morphology of biological samples, and combines autofluorescence lifetime and spectral information to realize a novel device for early diagnosis of cancer. The device includes a laser scanning confocal microscope device and a two-way fluorescence signal acquisition device. Different detectors respectively receive fluorescence lifetime information and fluorescence spectrum information, and use a computer processing system to process autofluorescence lifetime information and fluorescence spectrum information. The device can directly detect multi-dimensional information such as fluorescence images, fluorescence lifetimes, and fluorescence spectra of biological samples to be tested, and realize early detection and diagnosis of various cancers. It has broad application prospects in the fields of biomedicine and clinical diagnosis.

Description

technical field [0001] The invention belongs to the technical field of medical equipment, and in particular relates to a device for early diagnosis of cancer. Background technique [0002] Optical diagnosis is an important part of biomedical photonics, which uses optical methods to examine and analyze diseases. The techniques of optical diagnosis can be divided into two categories: tissue spectral diagnosis and tissue optical imaging diagnosis. Laser-induced autofluorescence spectroscopy is an effective optical diagnostic method. Compared with normal tissues, the biomolecular components of human cancerous tissues are changed, so the fluorescence emitted is different. The use of laser-induced fluorescence spectroscopy is suitable for collecting data on metabolism or structure and function, so as to achieve the purpose of diagnosing cancer. The use of fluorescence spectroscopy technology can provide experimental basis for early diagnosis of cancer and provide reference for s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 糜岚王梦妍潘晓波宋川
Owner FUDAN UNIV
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