Electrophoretic in situ tissue staining
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[0036] Electrophoretic Tissue Staining using Anti-CD34 Antibody in Tonsil.
[0037] The following experiment was run to determine if antibody could be introduced electrophoretically into tissue. The tissue was adhered to a hydrophilic polytetrafluoroethylene (PTFE) membrane (TEFLON® Plumber's Tape) to enable manipulation and orientation of the tissue in the gel, and then embedded in an agarose gel for subsequent electrophoresis.
[0038] Procedure: four sections of 5 μm-thick human tonsil were mounted to PVA-treated hydrophilic PTFE membrane, air dried for 48 hours, overnight dried at 60° C., manually de-paraffinized and re-hydrated (standard process of dipping sections sequentially in xylene, then 100% EtOH, 90% EtOH, 80% EtOH, 70% EtOH, and finally 100% H2O). The PTFE membrane was made hydrophilic by wetting in Isopropyl alcohol first, then soaking for several hours in a solution of 0.1% polyvinyl alcohol in phosphate buffer, pH 2.2 and 5% glutaraldehyde, and rinsed in DI water. Any h...
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