43 results about "Tissue Stains" patented technology

An automated staining system and a reagent container designed for use with the automated staining apparatus. The reagent container includes a reagent containment section capable of containing a volume of a reagent. The reagent containment section includes an upper wall and a base wall that are spaced apart along an axis. The base wall includes a well having a nadir that is aligned axially with an access opening in the upper wall so that a reagent probe entering the opening parallel to said axis will travel toward the nadir. In another aspect of the invention, the reagent container may include a two-dimensional data element containing reagent information. The staining apparatus may include one removable drawer for holding reagent containers and another removable drawer holding slides.

An embodiment of the method of the invention is a method of automating information flow in a laboratory performing tissue staining comprising positioning a networked label printer adjacent to a cutting station, the printer configured to access patient data directly or indirectly from the hospital LIS, the printer being configured with a data element scanner in electronic communication with said printer; inputting data from a tissue cassette-associated data element at said printer, whereby inputting data comprises reading the data from the cassette-associated data element and uploading the cassette data to the LIS; identifying the corresponding test protocol identifier and then downloading the test protocol data to the printer; printing information on labels corresponding to each test specified in the LIS for the patient; attaching a single label to each slide; and cutting a tissue section for each labeled slide and mounting the section on the slide.

Protein recognized by an antibody used as an active ingredient of an antibody medicine such as trastuzumab or an antibody used for targeting a target site of an active ingredient is highly accurately quantitatively determined by employing a quantitative tissue staining method of biological tissues, thereby providing a method for determining therapeutic effectiveness of a medicine containing such an antibody as a component. The effectiveness of a medicine containing an antibody as a component is determined by employing a tissue staining method comprising the steps of: labeling the antibody in the medicine containing an antibody as a component with a fluorescent material and contacting the thus fluorescence-labeled antibody with a tissue sample; obtaining a fluorescence image by irradiating, with excitation light, a tissue site contacted with the antibody; obtaining an autofluorescence image in the same field of view and at the same focus as in the fluorescence image in a close region on a shorter wavelength side or a longer wavelength side of an acquisition wavelength region of fluorescence emitted by the fluorescent material; obtaining a corrected fluorescence image by performing image processing for removing fluorescence brightness of the autofluorescence image from fluorescence brightness of the fluorescence image; counting the number of cells in the tissue site contacted with the antibody; measuring average fluorescence brightness per fluorescent particle; and calculating the number of fluorescent particles per cell.

The present invention introduces a radically different way of accelerating biomolecule conjugates into tissue, and hence towards their targets for purposes of tissue staining. The invention provides for an order of magnitude improvement over the prior art diffusion process used to stain tissue. The invention comprises a method of tissue staining by applying an electric field to a tissue sample in the presence of an electrolyte and biomolecular conjugates of interest suspended in the electrolyte. Typical staining times are reduced to seconds as opposed to 30-120 minutes common in the prior art. The invention is also directed to devices for performing the method.

A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

A highly accurate and quantitative method for determining cancer onset or cancer onset risk by a quantitative tissue staining method in biological tissues using an antibody capable of recognizing a cancer growth regulatory factor or cancer metastasis regulatory factor such as PAR1 antibody, which inhibits the cancer cell mobility and infiltration is provided. Cancer onset or cancer onset risk is determined using the tissue staining method comprising the steps of: labeling an antibody which recognizes a cancer growth regulatory factor or cancer metastasis regulatory factor with a fluorescent material, and contacting the fluorescent-labeled antibody with a tissue sample; irradiating a tissue site in contact with the antibody with excitation light to acquire a fluorescence image; acquiring an autofluorescence image in a vicinity region of a short wavelength side or long wavelength side of an acquisition region of fluorescence wavelength emitted by the fluorescent material, in the same field of vision and in the same focal point as those of the fluorescence image; acquiring a corrected fluorescence image by image processing to eliminate a fluorescent brightness of the autofluorescence image from the fluorescent brightness of the fluorescence image; counting the number of cells at the tissue site in contact with the antibody; measuring a mean fluorescent brightness of a single fluorescent particle; and calculating the number of fluorescent particles per cell.

Systems and methods for accelerating tissue processing by treating tissue samples and one or more tissue processing agents with infrasonic vibrations are discussed. Some non-limiting examples of tissue processing agents include a tissue fixative, dehydrating agent, clearing agent, impregnating agent, embedding agent, tissue stain, enzyme, or another chemical that diffuses into the tissue sample when the sample is being preserved or prepared for microscopic examination. The infrasonic vibrations can have a frequency from about 10 to about 600 Hz. The infrasonic vibrations can have an amplitude that is sufficiently high, when combined with the frequency, to induce turbulent mixing of the processing agent and accelerate tissue processing. The tissue sample may optionally be vibrated with ultrasonic vibrations. The ultrasonic vibrations can have a frequency and amplitude that are sufficiently high to induce turbulent mixing of the processing agent and to accelerate tissue processing.

An endoscopic stain is provided that includes a carbon pigment and a suspending/viscosity-increasing agent in a pharmaceutically acceptable delivery vehicle, wherein the carbon pigment has a total level of polycyclic aromatic hydrocarbons (PAH) of not greater than 0.5 ppm. Methods of staining an internal site utilizing the stain of the invention and kits that include the stain of the invention are also provided.

Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.

An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.

Provided is a method for staining a tissue enabling highly precise staining, by which the expression amount and/or the location of a biological substance in a tissue sample can be detected with a high quantitativity together with detailed information that can be obtained by bright field observation.

The tissue staining method of the present invention is a method for staining a tissue, in which both staining that allows bright field observation and fluorescence staining are carried out for the same specific biological substance.

An enhanced and particularly quick histological staining method is expressed in which a frozen tissue or deparafffinized tissue sample is briefly immersed in an eosin solution, used as a first dye, and then in a hematoxylin solution, used as a second die. The slide is rinsed and air-dried to make ready for assessment.

The invention relates to the field of new medical equipment, and in particular to a method and device for intelligent timing of a tissue staining machine. The method comprises the following steps of respectively obtaining the time required for the boom of the tissue staining machine to move vertically once and horizontally once; reading program information corresponding to the current staining process from the tissue staining machine, wherein the program information includes the position of each cylinder used in the current staining process and the corresponding staining time; and calculatingto obtain the total running time of the current staining process according to the time required for the boom of the tissue staining machine to move vertically once and horizontally once and the program information corresponding to the current staining process. The invention can predict the time required for the staining process.

The invention discloses a stain filter for an automatic tissue staining machine, which comprises a stain container connector, an infusion tube plug connector is arranged on the stain container connector, and a dye container connector is provided on the dye container connector. A filter is pluggably provided, and a first fixing rod and a second fixing rod are arranged on the connecting head of the dye container, and the first fixing rod and the second fixing rod clamp and fix the filter on the In the connecting head of the dye container, a fastening knob is respectively arranged on the first fixing rod and the second fixing rod. Since the dye filter of the automatic tissue staining machine of the present invention is provided with a pluggable filter sheet on the body, the filter sheet is clamped and fixed in the filter by two fixing rods arranged on the body, when the filter sheet When it needs to be replaced, the filter can be disassembled and replaced by loosening the fastening knob and opening the fixing rod, without dismantling and replacing the filter as a whole, which is very convenient to operate.