36 results about "Tissue Stains" patented technology

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

The invention relates to the technical field of optics, and discloses a visualization method of a biological tissue three-dimensional full-information atlas, which comprises the following steps of: acquiring image data, electron microscope data, mechanical data, protein distribution, omics information and histological staining images of biological tissues by using various biological visualization technologies, and correspondingly processing the acquired data to obtain a three-dimensional full-information atlas of the biological tissues; finally, organization information fusion and digital display are achieved. According to the method, a cross-scale feature correlation algorithm framework is innovatively established, and multi-source heterogeneous data fusion is performed on medical image topological data (CT / MRI), ultrastructure scanning data (SEM / TEM), molecular positioning information (immunofluorescence, immunohistochemistry and enzyme immunoassay), omics maps (space transcriptome / proteome) and high-resolution tissue staining data. The data island dilemma caused by traditional single-mode analysis is broken through, and full-scale visualization from nanoscale molecular distribution to centimeter-level organ tissue structure-function relationship is realized for the first time.

A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2 / a2+y2 / b2=1, where a=0.21, b=0.135, the rotation is −0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

The application discloses a method for generating protein expression information based on tissue staining images, comprising: inputting a tissue staining image to be analyzed into a trained neural network model, and generating protein expression information of at least one protein channel through inference of the neural network model, wherein the neural network model is trained in the following manner: cross-modal feature alignment training is performed on a tissue feature coding network in a neural network model to be trained, so that tissue structure image features are aligned with protein expression image features, to obtain a trained tissue feature coding network; the trained tissue feature coding network and a decoding network in the neural network model to be trained are taken as the neural network model to be trained, and protein expression supervised training is performed, to obtain the trained neural network model. The application reduces complex experimental steps and equipment requirements, can significantly reduce experimental costs and improve the efficiency of obtaining protein expression information.

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample. 20 25 20 31 55 02 M ay 2 02 5 A B S T R A C T 2 0 2 5 2 0 3 1 5 5 0 2 2 0 2 5 M a y

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

Disclosed herein is a method of analysing a tissue sample. The method comprises identifying one or more regions of interest within the tissue sample based on a tissue stain that has been applied to the tissue sample. Analyte material is then generated from the one or more regions of interest identified based on the tissue stain using a direct surface sampling probe (10), which analyte material is then received at a sampling inlet (30) and passed towards a mass and / or ion mobility spectrometer (50) for analysis.

A system comprising: a turntable including a plurality of mounting stations sized to receive at least one fluid dispensing cartridge; and a receiving assembly positioned below the turntable, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body including length and width dimensions of a chamber together defining a cavity for receiving a single slide; and a cover including a first position covering the cavity and a second position exposing a portion of the cavity. A method comprising moving the cover of a reaction station to expose a single microscope slide containing a sample, and dispensing one or more reagents onto the sample.

A tissue staining device (100) comprises: (a) a microscope slide carousel (105), wherein the microscope slide carousel (105) is configured to rotate on a first axis of rotation (230), and wherein the microscope slide carousel (105) comprises at least one microscope slide cartridge stall (106), and (b) a reagent assembly (110) comprising: (i) a reagent carousel (113), wherein the reagent carousel (113) is configured to rotate on a second axis of rotation (310), and wherein the reagent carousel (113) comprises at least one reagent holder, (ii) a dispensing actuator (115), and wherein the reagent carousel (113) is positioned above the microscope slide carousel (105), and wherein the reagent carousel (113) and the microscope slide carousel (105) are positioned such that when a reagent is placed in the reagent carousel (113) the reagent can flow from the reagent carousel (113) to the microscope slide carousel (105).

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample. 20 25 23 41 61 17 S ep 2 02 5 2 0 2 5 2 3 4 1 6 1 1 7 S e p 2 0 2 5 A B S T R A C T

The invention provides a tissue staining method based on combination of antigen repair treatment and silver staining, which comprises the following steps: a) performing high-temperature thermal repair on a to-be-treated tissue slice by using an antigen repair liquid, cooling, and cleaning with water to obtain a repaired tissue slice; and b) performing silver staining on the repaired tissue slice obtained in the step a), dehydrating and sealing the slice to obtain the stained tissue slice. Compared with the prior art, the tissue staining method based on combination of antigen repair treatment and silver staining provided by the invention adopts specific process steps and conditions to realize relatively good overall interaction: firstly, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, so that the signal-to-noise ratio of an age pigment region is remarkably improved; compared with the prior art, an ideal staining effect is obtained, detail visibility in pathological detection can be effectively improved, the method helps to more accurately evaluate the morphology and distribution characteristics of the age pigment, and the method has a wide application prospect.

To provide a biological tissue staining reagent, a biological tissue staining kit, and a biological tissue staining method in which an antibody can be sufficiently impregnated into a tissue.SOLUTION: A biological tissue staining reagent includes a peptide having a second amino acid sequence at least four amino acid of which matches with a first amino acid sequence of epitope in an antigen in a biological tissue being a target of immunostaining by an antibody.SELECTED DRAWING: Figure 5

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

Techniques for obtaining a synthetic histochemically stained image from a multiplexed immunofluorescence (MPX) image may include producing an N-channel input image that is based on information from each of M channels of an MPX image of a tissue section, where M and N are positive integers and N is less than or equal to M; and generating a synthetic image by processing the N-channel input image using a generator network, the generator network having been trained using a training data set that includes a plurality of pairs of images. The synthetic image depicts a tissue section stained with at least one histochemical stain. Each pair of images of the plurality of pairs of images includes an N-channel image, produced from an MPX image of a first section of a tissue, and an image of a second section of the tissue stained with the at least one histochemical stain.

The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. The method provided by the application detects and calculates the staining quality after each staining, adjusts the staining processing time of hematoxylin and eosin according to the staining quality, and replaces the staining solution if the staining processing time of hematoxylin and eosin exceeds the preset maximum value. The method provided by the application can effectively prolong the service life of the staining reagent, and can also ensure the uniformity and stability of pathological section staining.

This invention relates to the field of soft tissue biomechanical modeling technology, specifically to a constitutive modeling method suitable for J-type soft tissue. The method includes: processing the soft tissue using tissue staining to obtain the volume fractions of matrix, collagen fibers, and elastic fibers; determining an isotropic matrix term based on the characteristics of mesenchymal cells and endothelial cells in the matrix; determining a collagen fiber term based on the characteristics of collagen fiber curling, straightening, fiber rotation, and interfiber slippage; determining an elastic fiber term based on the fiber rotation characteristics of elastic fibers, thus obtaining a model of undetermined parameters; adding the volume fractions of matrix, collagen fibers, and elastic fibers in the soft tissue to the model of undetermined parameters; and using a nearest-nearest global optimization differential evolution algorithm to solve for the undetermined parameters in the model of undetermined parameters, thereby obtaining a hyperelastic constitutive model. This invention can improve the accuracy of the biomechanical property model of J-type soft tissue.

An autonomous quality and hallucination assessment method (AQuA) is disclosed, designed for either virtual tissue staining or histochemical staining. AQuA uses a trained neural network to autonomously achieve 99.8% accuracy when detecting acceptable and unacceptable virtually stained tissue images without access to ground truth, also presenting an agreement of 98.5% with the manual assessments made by a group of board-certified pathologists. AQuA achieves super-human performance in identifying realistic-looking, virtually stained hallucinatory images that would normally mislead human diagnosticians. The wide adaptability of AQuA is demonstrated across various virtually and histochemically stained human tissue images and showcase its strong external generalization to detect unseen hallucination patterns of virtual staining network models as well as artifacts observed in the traditional histochemical staining workflow.

The invention relates to a method for obtaining pathological diagnosis according to a color ultrasound image of a thyroid nodule by applying an artificial intelligence technology. The thyroid nodule color Doppler ultrasound image-pathological diagnosis system has the functions of image acquisition, image preprocessing, feature extraction, image recognition and the like, and can recognize, extract and memorize the color Doppler ultrasound image features of thyroid nodules and the internal features of abnormal cells in pathological tissue staining pictures of the thyroid nodules. Color Doppler ultrasound image data of thyroid nodules directly correspond to pathological picture data, and the data can be interpreted. A color Doppler ultrasound image database and a pathological picture database of thyroid nodules are established, large-scale machine learning is carried out with sufficient data, and functions of the machine learning are improved. The system is verified and clinically tested to ensure the accuracy of the system, and pathological diagnosis is displayed. The method has the advantages that the pathological diagnosis can be obtained when nodules are seen on the thyroid color ultrasound, and the purpose of accurate treatment can be achieved. Finally, clinical diagnosis is based on pathological results under a microscope.

1. Name of the Design Product: Biological Tissue Staining Chip. 2. Use of the Design Product: The biological tissue staining chip is used to carry biological tissue sections and stain them. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective View 1. 5. Other Situations Requiring Explanation: In Perspective View 1, Perspective View 2, Front View, Enlarged View of Part A and Enlarged View of Part B, the gray-coated areas represent the transparent structure of this design, and the gray color is not claimed for protection.

The utility model discloses a tissue staining jar, which belongs to the technical field of medical auxiliary instruments and comprises a staining jar, and a detachable jar cover is arranged at the top of the staining jar. The dyeing device comprises a dyeing cylinder, a separation frame body is arranged in the dyeing cylinder, the separation frame body is in sliding fit with the inner wall of the dyeing cylinder, a plurality of dyeing cavities are formed in the separation frame body, a separation plate is arranged in each dyeing cavity, and the separation plates are in inserted fit with the dyeing cavities. According to the utility model, the separation frame body can be detached from the staining jar, the separation plate can be detached from the staining cavity, and the staining jar, the separation frame body and the separation plate can be cleaned and disinfected after the staining of a glass slide is completed, so that cross contamination and impurity interference removal are avoided.

The waste gas treatment device comprises a pathological tissue dyeing machine body, a shell is arranged on the left side of the pathological tissue dyeing machine body, an air outlet and an air inlet are formed in the left side face and the right side face of the shell respectively, and the air inlet is communicated with the interior of the pathological tissue dyeing machine body. A primary filtering cavity, a chemical filtering cavity and a light filtering cavity which are communicated with one another are sequentially formed in the shell from right to left, and a primary filtering assembly, a chemical filtering assembly and a light filtering assembly are arranged in the primary filtering cavity, the chemical filtering cavity and the light filtering cavity respectively; the primary filtering cavity and the light filtering cavity are respectively communicated with the air inlet and the air outlet, and the air inlet and the air outlet are respectively provided with an air inlet fan and an exhaust fan; the multi-stage filter has the advantage of multi-stage filtration.

The invention relates to a method for obtaining pathological diagnosis according to color ultrasound images of breast nodules by applying an artificial intelligence technology. The breast nodule color ultrasound image-pathological diagnosis system has the functions of image acquisition, image preprocessing, feature extraction, image recognition and the like, and can be used for recognizing, extracting and memorizing color ultrasound image features of breast nodules and internal features of abnormal cells in pathological tissue staining pictures of the breast nodules. Color Doppler ultrasound image data of breast nodules directly correspond to pathological picture data, and the data can be interpreted. A color Doppler ultrasound image database and a pathological picture database of breast nodules are established, deep learning is carried out with sufficient data, and functions are improved. The system is verified and clinically tested to ensure the accuracy of the system. Pathological diagnosis can be displayed after color Doppler ultrasound image data of breast nodules are input into the system. The device has the advantages that pathological diagnosis can be judged when nodules are seen on breast color ultrasound, so that the aim of accurate treatment is fulfilled. Clinical final diagnosis is based on pathological results under a microscope.

1. Name of the Design Product: Tissue Stainer. 2. Use of the Design Product: This design product is specifically applied in the fields of bioengineering, cell or tissue staining. 3. Design Key Points of the Design Product: It lies in the combination of shape and color. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view. 5. The claimed design includes color.

A sample processor includes: a body including an inner surface defining a chamber having an opening in a side of the body and including a volume therein for receiving at least one microscope slide; a door including a first position covering the opening of the body and a second position exposing a portion of the chamber through the opening; a slide bed disposed in the chamber; an internal humidity generator disposed in the chamber and one of an external humidity generator coupled to the chamber. A system includes at least one sample processor, at least one reagent outside the body of the at least one sample processor, and a method comprising: subjecting a sample on a microscope slide in a sealed chamber to pressure greater than ambient and humidity greater than 60%; and processing the sample.

Diagnosis based on antigen-specific detection can be performed by other types of tissue staining with lower cost and wider availability.SOLUTION: The present disclosure relates to techniques for obtaining composite immunohistochemistry (IHC) images from histochemical stain images. Among other things, aspects of the present disclosure are directed to accessing an input image depicting a tissue section that has been stained with at least one histochemical stain, generating a synthetic image by processing the input image using a trained generator network, and outputting the synthetic image. The composite image depicts a tissue section that has been stained with at least one IHC stain directed to a first antigen, and the techniques may also include receiving an input based on an expression level of the first antigen from the composite image and / or generating a value based on the expression level of the first antigen from the composite image.SELECTED DRAWING: Figure 15A

Provided is a tissue staining composition containing carbon particles having a mean particle diameter less than 0.3 μm in diameter (optionally less than 0.2 μm in diameter) together with one or more agents that maintain the carbon particles in suspension (for example, an anti-settling agent and / or surfactant). In certain circumstances, the anti-settling agent may also have mucoadhesive properties. The tissue staining composition is visually dark and does not disperse rapidly when introduced into regions of tissue of interest making it ideal for marking the regions that can be visualized clearly and over prolonged periods of time via, for example, direct visualization, endoscopic or laparoscopic inspection. The invention also provides methods of making and using the tissue staining composition for marking regions of tissue of interest, for example, gastrointestinal tissue, as well as other tissues.

To provide a method, a system and a program which facilitate acquisition of a synthesized histological coloring image from a multiplex immunofluorescent (MPX) image.SOLUTION: A method according to the present invention has a step of generating a N-channel input image based on information from each of M channels of a MPX image of a tissue slice (M and N are positive integers and satisfy N≤M), and a step of generating a synthesized image by processing the N-channel input image by using a network of generators trained by using a training data set including a plurality of image pairs. The synthesized image indicates a tissue slice dyed by at least one histological chemical coloring agent. Each of the plurality of image pairs includes the N-channel image generated from a MPX image of a first slice of the tissue and an image of a second slice of the tissue colored by the at least one histological chemical agent.SELECTED DRAWING: Figure 8A