20 results about "Tissue Stains" patented technology

The invention relates to a CACNA1A-based mental patient heart death intelligent identification system and method, the system comprises an immunohistochemical staining device, an image acquisition unit, an image analysis unit and an intelligent identification unit, the immunohistochemical staining device is used for performing CACNA1A immunohistochemical staining on formalin-fixed paraffin-embedded heart tissue slices; the image acquisition unit is used for shooting and obtaining a heart tissue CACNA1A immunohistochemical staining image; the image analysis unit is used for processing the heart tissue CACNA1A immunohistochemical staining image so as to determine a tissue staining region and image features thereof; and the intelligent identification unit outputs an identification prediction result of heart death of the mental patient according to the image features of the tissue staining region. Compared with the prior art, the mental patient heart death identification system can intelligently and accurately identify mental patient heart death.

A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2 / a2+y2 / b2=1, where a=0.21, b=0.135, the rotation is −0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

The application discloses a method for generating protein expression information based on tissue staining images, comprising: inputting a tissue staining image to be analyzed into a trained neural network model, and generating protein expression information of at least one protein channel through inference of the neural network model, wherein the neural network model is trained in the following manner: cross-modal feature alignment training is performed on a tissue feature coding network in a neural network model to be trained, so that tissue structure image features are aligned with protein expression image features, to obtain a trained tissue feature coding network; the trained tissue feature coding network and a decoding network in the neural network model to be trained are taken as the neural network model to be trained, and protein expression supervised training is performed, to obtain the trained neural network model. The application reduces complex experimental steps and equipment requirements, can significantly reduce experimental costs and improve the efficiency of obtaining protein expression information.

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample. 20 25 20 31 55 02 M ay 2 02 5 A B S T R A C T 2 0 2 5 2 0 3 1 5 5 0 2 2 0 2 5 M a y

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample.

Disclosed herein is a method of analysing a tissue sample. The method comprises identifying one or more regions of interest within the tissue sample based on a tissue stain that has been applied to the tissue sample. Analyte material is then generated from the one or more regions of interest identified based on the tissue stain using a direct surface sampling probe (10), which analyte material is then received at a sampling inlet (30) and passed towards a mass and / or ion mobility spectrometer (50) for analysis.

A system comprising: a turntable including a plurality of mounting stations sized to receive at least one fluid dispensing cartridge; and a receiving assembly positioned below the turntable, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body including length and width dimensions of a chamber together defining a cavity for receiving a single slide; and a cover including a first position covering the cavity and a second position exposing a portion of the cavity. A method comprising moving the cover of a reaction station to expose a single microscope slide containing a sample, and dispensing one or more reagents onto the sample.

A system including: a carousel including a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge; and a receiving assembly positioned beneath the carousel, the receiving assembly including a plurality of reaction stations, each of the plurality of reaction stations including: a body comprising a length dimension and a width dimension that together define a chamber to accommodate a single slide therein; and a lid comprising a first position to cover the chamber and a second position to expose a portion of the chamber. A method including moving a lid of a reaction station to expose a single microscope slide including a sample and dispensing one or more reagents on the sample. 20 25 23 41 61 17 S ep 2 02 5 2 0 2 5 2 3 4 1 6 1 1 7 S e p 2 0 2 5 A B S T R A C T

To provide a biological tissue staining reagent, a biological tissue staining kit, and a biological tissue staining method in which an antibody can be sufficiently impregnated into a tissue.SOLUTION: A biological tissue staining reagent includes a peptide having a second amino acid sequence at least four amino acid of which matches with a first amino acid sequence of epitope in an antigen in a biological tissue being a target of immunostaining by an antibody.SELECTED DRAWING: Figure 5

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

Techniques for obtaining a synthetic histochemically stained image from a multiplexed immunofluorescence (MPX) image may include producing an N-channel input image that is based on information from each of M channels of an MPX image of a tissue section, where M and N are positive integers and N is less than or equal to M; and generating a synthetic image by processing the N-channel input image using a generator network, the generator network having been trained using a training data set that includes a plurality of pairs of images. The synthetic image depicts a tissue section stained with at least one histochemical stain. Each pair of images of the plurality of pairs of images includes an N-channel image, produced from an MPX image of a first section of a tissue, and an image of a second section of the tissue stained with the at least one histochemical stain.

The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. The method provided by the application detects and calculates the staining quality after each staining, adjusts the staining processing time of hematoxylin and eosin according to the staining quality, and replaces the staining solution if the staining processing time of hematoxylin and eosin exceeds the preset maximum value. The method provided by the application can effectively prolong the service life of the staining reagent, and can also ensure the uniformity and stability of pathological section staining.

A sample processor includes: a body including an inner surface defining a chamber having an opening in a side of the body and including a volume therein for receiving at least one microscope slide; a door including a first position covering the opening of the body and a second position exposing a portion of the chamber through the opening; a slide bed disposed in the chamber; an internal humidity generator disposed in the chamber and one of an external humidity generator coupled to the chamber. A system includes at least one sample processor, at least one reagent outside the body of the at least one sample processor, and a method comprising: subjecting a sample on a microscope slide in a sealed chamber to pressure greater than ambient and humidity greater than 60%; and processing the sample.

Diagnosis based on antigen-specific detection can be performed by other types of tissue staining with lower cost and wider availability.SOLUTION: The present disclosure relates to techniques for obtaining composite immunohistochemistry (IHC) images from histochemical stain images. Among other things, aspects of the present disclosure are directed to accessing an input image depicting a tissue section that has been stained with at least one histochemical stain, generating a synthetic image by processing the input image using a trained generator network, and outputting the synthetic image. The composite image depicts a tissue section that has been stained with at least one IHC stain directed to a first antigen, and the techniques may also include receiving an input based on an expression level of the first antigen from the composite image and / or generating a value based on the expression level of the first antigen from the composite image.SELECTED DRAWING: Figure 15A

The invention discloses a micro-fluidic chip, a tissue dyeing device comprising the micro-fluidic chip and a tissue dyeing method. The micro-fluidic chip comprises an upper slide, wherein the upper slide is provided with an inlet hole and an outlet hole; the double-faced adhesive tape layer is used for removing part of double-faced adhesive tape through cutting so as to present an inflow channel, a dyeing cavity and an outflow channel of the corresponding micro-fluidic chip, the inflow channel is communicated with the inlet hole, and the outflow channel is communicated with the outlet hole; a to-be-dyed tissue is arranged on the lower slide; wherein the upper slide and the lower slide are adhered into a whole through the double-sided adhesive layer. A reagent bin is arranged at an inlet of the micro-fluidic chip, the reagent bin is designed to be open, and an outlet of the micro-fluidic chip is connected with a pressure pump. A reagent in the reagent bin is pumped into the dyeing cavity of the micro-fluidic chip through the pressure pump for dyeing, and is discharged after dyeing. According to the invention, the complexity and cost of the system are reduced, and meanwhile, the waste of reagents is reduced.

A sample processor including a body including an inner surface that defines a chamber including an opening in a side of the body and including a volume to accommodate a least one microscope slide therein; a door comprising a first position to cover the opening of the body and a second position to expose a portion of the chamber through the opening; a slide bed disposed in the chamber; one of an internal humidity generator disposed in the chamber and an external humidity generator coupled to the chamber. A system including at least one sample processor at least one reagent outside the body of the at least one sample processor and a method including subjecting a sample on a microscope slide in a sealed chamber to a pressure greater than ambient and a humidity greater than 60 percent; and processing the sample.

A rapid fresh digital-pathology (RFP) method for assessing an excised unfixed biological specimen stained with hematoxylin (H) or eosin (E) or both hematoxylin and eosin (HE) staining dyes. The RFP method is assisted by a rapid tissue staining (RTS) procedure which is performed on the excised unfixed biological specimen, involving a short fixation; an H-staining; a rinsing; a bluing; an E-staining; a rinsing; and finally, a covering of a stained specimen with a coverslip. The RFP method is further assisted by a multimodal nonlinear optical laser-raster-scanning approach to provide with a nonlinear multi-harmonic generation and / or a nonlinear multi-photon excitation fluorescence signal(s) for multichannel digitization and real-time digital display of H- or E- or HE-specific histopathological features while providing a centimeter-scale imaging area, a submicron digital resolution, and a sustained effective data throughput of at least 500 Megabits per second (Mbps).