Cell tissue dyeing method and dyeing machine

Abstract

The invention provides a cell tissue staining method which can keep a slide still and control the flow mode of a staining solution, and a staining machine thereof. The staining machine mainly comprises a liquid storage tank, a solenoid valve, a dye vat, a machine frame, a control system, a transition block and a liquid collection device; the liquid storage tank is positioned at the upper part of the machine frame; the dye vat is positioned at the lower part of the machine frame; the liquid collection device and the solenoid valve are positioned between the liquid storage tank and the dye vat; the liquid storage tank is connected with the liquid collection device through the solenoid valve; and the liquid collection device is connected with the dye vat through the transition block. The method is characterized in that the natural liquid level pressure difference of the staining solution serves as a liquid working power source during the entire process of staining; the staining solution in the liquid storage tank flows into the liquid collection device through the solenoid valve; the staining solution in the liquid collection device is heated to the temperature set for staining, and then flows into the dye vat provided by a slide through the transition valve; and the staining process is completed by the slide in the dye vat. Compared with the traditional staining machine which keeps staining solution still and controls the model of slide movement, the invention is advanced and can achieve the effects of enhancing staining effect, improving staining efficiency, saving energy, reducing pollution and saving staining cost.

Description

technical field [0001] The invention relates to a cell and biological tissue staining method and equipment in the technical fields of medical care and biological research, in particular to a cell tissue staining method and a staining machine. Background technique [0002] So far, there are relatively few cell and biological tissue staining methods and equipment types. The cell tissue staining equipment used on the market is mainly to place the slides coated with cell tissue in various dyeing solutions (dyes and auxiliary liquids are called dyeing liquids). In the dyeing vats of liquid), a group of glass slides is controlled by a mechanical device, moved to the top of each dyeing vat according to the requirements of the staining program, and then vertically lifted, the slides are soaked in the dyeing solution, and the above actions are repeated after the soaking time is reached. [0003] This dyeing method and device have the following disadvantages, especially when there are...

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Problems solved by technology

[0003] This dyeing method and device has the following disadvantages, especially when there are many dyeing steps

[0004] 1. Since the dye solution cannot be replaced in time, the effective components of the dye solution gradually decrease before the dye solution is exhausted, the dyeing effect is extremely poor, and the dyeing effect before and after is inconsistent;

[0005] 2. Since it is necessary to control the temperature of each dyeing vat, and to control the translation and lifting of the glass slide (rack), the energy consumption is high, the noise is large, and the equipment is too large and the cost is high;

[0006] 3. Only one kind of dyeing can be done in the dyeing process. If another kind of dyeing is needed, multiple dyeing solutions need to be replaced, and the operation is complicated

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