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Manufacturing method of permanent microscopic slide specimen for tiny insects

A technique for making microscopic slides and specimens, which is applied in the field of insect specimens and achieves the effects of good staining effect, simple steps and reduced complexity.

Inactive Publication Date: 2021-06-04
SHAANXI SCI TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for making a permanent microscopic slide specimen of tiny insects, simplify the production process of the microscopic slide specimen, and accurately measure the type and concentration of the solution, as well as its use temperature and time, To achieve the quality control of specimen production, avoid air bubbles in the cover, shrinkage and deformation of the insect body structure during dehydration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1, placing the living body of brown planthopper in 65% alcohol solution for soaking;

[0028] Step 2, take the brown planthopper out of the alcohol solution, cut the body, put it into a beaker with 5% sodium hydroxide solution, place the beaker on a heating plate and heat it at 120°C for 20 minutes, and clean the contents and viscera until the brown planthopper The worm body becomes transparent;

[0029] Step 3, taking out the brown planthopper body treated in step 2 from the sodium hydroxide solution, and putting it into the acid fuchsin alcohol solution in a constant temperature cold bath with an alcohol solvent concentration of 30%, 55%, 65%, and 90% for 40 minutes, The concentration of alcohol solvent is 100% in acid fuchsin alcohol solution in constant temperature cold bath for 100min to complete the color fixation of insect body;

[0030] Step 4, remove the brown planthopper body treated in step 3 and place it on filter paper, and place it in a vacuum chambe...

Embodiment 2

[0034] Step 1, soaking the wheat aphid living body in 70% alcohol solution;

[0035] Step 2, take the wheat aphid out of the alcohol solution, cut the body, put it into a beaker with 5% sodium hydroxide solution, place the beaker on a heating plate and heat it at 100°C for 20 minutes, and clean the contents and viscera until The worm body becomes transparent;

[0036] Step 3, take out the wheat aphid worm body after the step 2 treatment from the sodium hydroxide solution, and put it into the acid fuchsin alcohol solution in constant temperature cold bath with alcohol solvent concentration of 30%, 55%, 65%, and 90% for 50min in turn 1. The concentration of alcohol solvent is 100% constant temperature cold bath acid fuchsin alcohol solution for 120min to complete the color fixation of wheat aphids;

[0037] Step 4, remove the wheat aphid body treated in step 3 and place it on filter paper, and place it in a vacuum chamber with a constant temperature of 25°C to dry and dehydrate...

Embodiment 3

[0041] Step 1, soaking the live scale insect in 76% alcohol solution;

[0042] Step 2, take the scale insect out of the alcohol solution, cut the body, put it into a beaker with 5% sodium hydroxide solution, place the beaker on a heating plate and heat it at 100°C for 20 minutes, and clean the contents and viscera until The scale insect body becomes transparent;

[0043] Step 3, taking out the scale insect bodies treated in step 2 from the sodium hydroxide solution, and putting them into the acid fuchsin alcohol solution in a constant temperature cold bath with an alcohol solvent concentration of 30%, 55%, 65%, and 90% for 45 minutes 1. The concentration of alcohol solvent is 100% constant temperature cold bath acid fuchsin alcohol solution for 100min to complete the color fixation of scale insects;

[0044] Step 4, removing the scale insects treated in step 3 and placing them on filter paper, and drying and dehydrating them in a constant temperature vacuum chamber at 25°C un...

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PUM

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Abstract

The invention discloses a manufacturing method of a permanent microscopic slide specimen for tiny insects. The manufacturing method comprises the steps of fixing, decolorizing, dehydrating, color fixing, drying and sealing. The manufacturing method has the beneficial effects that firstly, compared with an existing specimen manufacturing method, the manufacturing method of the slide specimen is simple in step, the used materials cannot cause environmental pollution, the dyeing effect is good, and the complexity of the specimen manufacturing process and the harm to a human body can be reduced; secondly, according to the manufacturing method of the slide specimen, specimen drying is achieved through a constant-temperature vacuum chamber, sufficient evaporation of a solution in the specimen is facilitated, and the problems that internal bubbles appear due to existing natural air drying and the surface of the specimen is turbid are solved; finally, according to the manufacturing method of the slide specimen, insect bodies are dyed through an acid fuchsin alcohol solution, the body structures of the insect bodies can be more obviously shown, and shrinkage and deformation of the insect body structures cannot be caused through layer-by-layer dehydration of alcohol with different concentrations.

Description

technical field [0001] The invention relates to the technical field of insect specimens, in particular to a method for making permanent microscopic slide specimens of tiny insects. Background technique [0002] When carrying out morphological research on small insects (such as scale insects, aphids, whiteflies, mites, etc.) Make it into a microscopic slide specimen, and directly observe the internal structure of the worm body through a microscope. Microscopic slides include: temporary and semi-permanent slides, and permanent slides. [0003] At present, common methods for making permanent microscopic slides include the Venetian turpentine method, the tert-butanol gum method, and the dioxane gum method. The above-mentioned method basically includes the following steps: decolorization, dehydration, transparency and sealing. However, the practical results show that the existing methods for making permanent microslides require accurate measurement of the type and concentratio...

Claims

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Application Information

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IPC IPC(8): A01N1/00G09B19/00
CPCA01N1/00G09B19/00
Inventor 党利红李彦巧王夏
Owner SHAANXI SCI TECH UNIV
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