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Peach rootstock south root-knot nematode resisting in-vitro rapid screening method

A technology of root knot nematode and screening method, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of long duration, unstable environmental conditions, complex statistics, etc., and achieve the effect of simple operation and novel design

Inactive Publication Date: 2015-10-14
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

However, no matter which method is used, it is necessary for root-knot nematodes to pull out the plant roots from the soil after a growth cycle, and then count the number or size of root knots after cleaning, and judge the resistance of plants according to the resistance standard. Instability, long duration, complex statistics, etc.

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  • Peach rootstock south root-knot nematode resisting in-vitro rapid screening method

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Embodiment

[0026] A method for in vitro rapid screening of peach rootstock resistance to root-knot nematode incognita, comprising the following steps:

[0027] (1) Seedling cultivation: Store the seeds of Beilei (high sensitivity) and Baigen Gansu peach (high resistance) in low-temperature sand for later use. After 3-4 months, the seeds germinate and are taken out for inoculation.

[0028] (2) Separation of second-instar root-knot nematodes: smash tomato roots infected with root-knot nematodes, pass through 20, 100, 200, and 500 mesh sieves respectively, collect worm eggs on 500 mesh sieves, and pass through the sucrose flotation method Remove impurities and concentrate eggs, pour the concentrated eggs into a petri dish covered with a 200-mesh sieve and two layers of filter paper, collect the second instar root-knot nematode after 3 days, centrifuge at 3000r / min for 3min, discard part of the supernatant and increase For the concentration of eggs, calculate the concentration of nematodes ...

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Abstract

The present invention discloses a peach rootstock south root-knot nematode resisting in-vitro rapid screening method. The method comprises the following steps of: culturing seedlings; separating 2-year-old root-knot nematodes; preparing 23% Pluronic F-127; inoculating: adopting a 6-pore cell culture plate; adding 5ml of the 23% Pluronic F-127 and 500 2-year-old root-knot nematodes into each hole and uniformly mixing; putting peach seedlings into the holes when gel is about to condense; covering by the peach seedlings with a preservative film to keep moisture, and culture; dyeing: dyeing root tips with a sodium hypochlorite-acid fuchsin-glycerinum method after inoculating for 30 hours, and tabletting after dyeing; and counting and identifying: counting up the quantity of the nematodes under a microscope and defining the disease resistance of plants according to infection quantity. The peach rootstock south root-knot nematode resisting in-vitro rapid screening method has a novel design, is simple to operate and is safe and convenient; and the problems in a test that the resistance identification of the plant root nematodes is not stable, the duration is long and the counting is complicated are solved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and relates to a rapid screening method for peach rootstock resistance to root-knot nematode in vitro. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is a kind of plant obligate endoparasitic nematode widely distributed in the world. It mainly occurs in temperate and tropical regions. economic loss. Root-knot nematode infection can cause damage to the root system of plants, significantly reducing the absorption of nutrients and water, weakening plant growth and reducing yield. Breeding and promoting disease-resistant varieties is the most cost-effective defense measure. [0003] In order to accurately identify root-knot nematode resistance of germplasm resources, researchers have established many resistance identification methods, which are mainly divided into two methods: outdoor or greenhouse pot plant identification. However, no matter which method is used, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 王力荣李海炎曹珂朱更瑞王新卫方伟超陈昌文
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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