12results about How to "High soluble solids content" patented technology

The invention discloses an application of a tomato B3-337 transcription factor in improvement of tomato quality, a gene for coding a B3-337 transcription factor protein is named as a B3-337 gene, and the nucleotide sequence is shown as SEQ ID NO.1. The invention also discloses an application of the tomato B3-337 transcription factor in improvement of tomato quality. According to the application, a carrier pFM3100GFP-B3-337 is constructed, so that the B3-337 is proved to be positioned in a cell nucleus. A transcriptional activity detection result proves that B3-337 has transcriptional activation activity. RT-qPCR (real-time quantitative polymerase chain reaction) and GUS (glucuronidase) dyeing show that the transcription factor is expressed in pericarp, seed, placenta and center pillar of each fruit ripening stage. According to the invention, a B3-337 gene overexpression plant is obtained by constructing a B3-337 overexpression vector, and compared with a wild type, the tomato fruit overexpressed with the B3-337 gene has the advantages that the content of soluble solids brix is increased, the fruit becomes larger, and the weight becomes heavier. It is shown that the B3-337 transcription factor plays an important regulation role in positive regulation of tomato fruit quality. The invention lays a good gene genetic resource foundation for the cultivation of new varieties of high-quality tomatoes.

The invention belongs to the technical field of thick-skin muskmelon cultivation, and particularly relates to a thick-skin muskmelon cultivation method under a simple rain sheltering facility. A simple rain sheltering facility comprises a plurality of greenhouse bodies arranged at intervals, each greenhouse body comprises a supporting stand column vertically fixed to the ground, arch-shaped rods are fixed to the tops of the supporting stand columns, and horizontal cross beams are fixed to the two ends of each arch-shaped rod; the thin film is laid on the tops of the arched rods of the greenhouse main body; and the vine hanging rope and the horizontal cross beams are located on the same horizontal plane, and the vine hanging rope is vertically fixed among the horizontal cross beams of the plurality of greenhouse main bodies. The transparent film is only laid on the top, no structure is arranged on the periphery, and the whole greenhouse body can be ventilated; secondly, compared with an existing common steel pipe greenhouse, the cost is greatly reduced; moreover, the thick-skin muskmelons are in a semi-open-air environment and can attract surrounding insects for pollination, the production link of artificial pollination is omitted, the labor cost is effectively reduced, meanwhile, the yield and quality of the thick-skin muskmelons are improved, and the method has extremely high popularization value.

The invention belongs to the technical field of plant growth regulators, and particularly relates to an application of a plant growth regulator containing compound sodium nitrophenolate and DCPTA in increasing the tomato yield and improving the tomato quality. Comprising the following components in parts by weight: 5-90 parts of compound sodium nitrophenolate, 15-270 parts of DCPTA, 50-400 parts of a solvent, 10-200 parts of an emulsifier, 0-60 parts of an anti-freezing agent, 0-10 parts of a preservative, 1-10 parts of a defoaming agent and the balance of water, wherein the weight ratio of the compound sodium nitrophenolate to the DCPTA is 1: 3. The plant growth regulator disclosed by the invention can effectively promote the expansion of tomato fruits, increase the flowering number, the young fruit number, the plant height, the single fruit weight, the maximum transverse diameter and the yield, achieve the purpose of increasing the yield, and also can improve partial nutritional quality (vitamin C content, soluble sugar and soluble solids) of the tomatoes to a certain extent, so that the yield of the tomatoes is increased. The content of titratable acid can be reduced to a certain extent, and the compound is safe to tomatoes, safe to the environment and small in residue.

The invention provides a microbial compound fertilizer for strawberries as well as a preparation method and application thereof, and belongs to the technical field of microbial fertilizers, the microbial compound fertilizer is prepared from the following raw materials in parts by weight: livestock manure, urea, monopotassium phosphate, mineral charcoal, distillers' grains, medical stone, a microbial agent and plant components; the microbial agent is prepared from pichia guilliermondii, trichoderma harzianum, bacillus subtilis and bacillus velezensis; the plant components comprise wild chrysanthemum flowers, artemisia annua, black nightshade herbs and flaccid knotweed herbs. Through the synergistic effect of the strains, growth of plant disease bacteria can be inhibited, growth of strawberry seedlings is promoted, absorption of strawberries is assisted by generated small molecular substances, the strawberry quality is improved, and the pesticide composition can be used for preventing and treating various strawberry diseases such as powdery mildew, gray mold, anthracnose and fusarium wilt; the content of soluble solids, the content of soluble sugar and the content of vitamin c in fruits are increased, the yield of strawberries is increased, and quality improvement and yield increase are facilitated.

The invention provides application of a GA3-containing accelerant in improving the yield and quality of kiwi fruits, and belongs to the technical field of fruit tree cultivation. The invention provides application of GA3 in improving the yield and quality of kiwi fruits. The working concentration of the GA3 is 50-100 mg / L. The invention further provides a GA3-containing accelerant, for example, after fruit soaking treatment is conducted through a solution containing 100 mg / L of GA3 and 5 g / L of CaCl2 in the embodiment, the single fruit quality of kiwi fruits can be remarkably improved, the soluble solid content, the ascorbic acid content, the soluble sugar content and the sugar-acid ratio of the fruits can be remarkably improved, the acidity of the fruits can be remarkably reduced, and the influence on the thickness of kiwi fruit peels is not large. Therefore, by utilizing the accelerant disclosed by the invention, the comprehensive quality of the kiwi fruits can be remarkably improved on the premise of increasing the fruit yield.

The application discloses application of HD-ZIP transcription factors in quality regulation of tomatoes, and the protein has functional redundancy, and the functions of the protein are jointly regulated by two genes, wherein a CDS sequence of the gene Solyc03g034130.2 is shown as SEQ ID NO. 1, and a CDS sequence of the gene Solyc03g034110.2 is shown as SEQ ID NO. 2. A tomato transcription factor mutant library is constructed through CRISPR technology, and a mutant Tom6-155 which simultaneously inhibits expression of two homologous genes of the HD-ZIP transcription factor is screened. Based on observation of a phenotype of the mutant, the inventors determine that the HD-ZIP transcription factor is related to tomato fruit quality. Compared with a wild type, the brix content of the tomato fruit of the mutant is increased. It is indicated that Solyc03g034130.2 and Solyc03g034110.2 play important negative regulation roles in a tomato fruit quality formation process. The application lays a good genetic resource foundation for cultivation of a new tomato variety with high quality.

The invention discloses a pollen suspension for improving the fruit shape and quality of a perfume pomelo as well as a preparation method and application of the pollen suspension. The pollen suspension is obtained by mixing a nutrient solution and Majia pomelo pollen; the nutrient solution is prepared from the following raw materials in parts by weight: 8 to 12 parts of xanthan gum, 5200 to 7800 parts of cane sugar, 36 to 54 parts of calcium gluconate, 4 to 6 parts of boric acid and 40000 to 60000 parts of water; the activity of the Majia pomelo pollen is not lower than 70%; the solid-to-liquid ratio of the Majia pomelo pollen to the nutrient solution is (0.2-0.6) g: 1L; the method can be used for improving the fruit shape and quality of the perfume pomelo, and compared with single-sexual fruiting in a natural state, the method can improve the fruit quality; the malformed fruit rate and the peel thickness of the perfume pomelo are reduced, the edible rate of fruits is improved, and the content of soluble solids in pulp is increased; compared with a traditional cross pollination mode, the method has the advantages that the seed characteristics can be optimized, the seed state of fruits can be obviously changed, and the industrial problem that quality improvement is accompanied by grain increase is solved; the pollination operation efficiency can be improved, and manual labor investment is saved.

The invention provides a method for increasing the soluble solid content of Guanjade loquat fruits, which comprises the following steps: at the end of March and the beginning of April every year, when young fruits of the Guanjade loquats are as large as peanuts, carrying out fruit homogenizing work, and reserving 2-3 fruits for each cluster; in the first ten days of March, a decomposed cake fertilizer, mineral source potassium fulvate and a first compound fertilizer are applied to the Guanjade loquat trees; within one week after the Guanjade loquats are harvested, the bio-organic fertilizer and the compound fertilizer II are applied to the Guanjade loquats. In the development process of the Guanjade loquat fruits, the soluble solid content of the Guanjade loquat is increased by adjusting the formula and the fertilization period of the Guanjade loquat fertilizer, the quality of the loquat fruits is improved, and meanwhile, the effect of increasing the efficiency and the income of Guanjade loquat planters is remarkable.

The application belongs to the field of preservation technology, and particularly relates to application of tea tree oil active ingredients in preparation of fruit and vegetable preservation preparation. The tea tree oil active ingredients are 4-terpineol, p-cymene or alpha-muurolene. The preparation is used for inhibiting common rot fungi of fruits and vegetables after harvesting, and the rot fungi include Alternaria spp., Botrytis spp., Penicillium spp., Fusarium spp. and the like. The preparation can also reduce softening of fruits and vegetables, and reduce the degree of membrane lipid peroxidation of fruit and vegetable tissues. The preparation is specifically a fumigation preparation or an atomization preparation. The tea tree oil active ingredients are used for fumigation treatment or atomization treatment of fruits and vegetables in a relatively closed space, and the treated fruits and vegetables are stored. The incidence of the treated fruits and vegetables is significantly reduced, aging is delayed, and the quality of the fruits is maintained.

This invention discloses a closed-loop soil improvement method for vineyards using organic fertilizer synergy, comprising the following steps: seasonal directional grass cultivation, preparation of Bacillus subtilis agent, preparation and application of self-made organic fertilizer in the early stage of vineyard establishment, and closed-loop synergistic improvement method; the closed-loop synergistic improvement method is divided into early stage improvement and late stage improvement, relying on grass return to the field and Bacillus subtilis to maintain soil fertility and nutrient cycle.