Method for testing toxicity of nanomaterial by using transgenic zebra fish embryo model

A technology of zebrafish embryos and nanomaterials, which is applied in the field of testing the toxicity of nanomaterials through the transgenic zebrafish embryo model, can solve the problems of cumbersome methods, long test period, and inability to describe the toxicity of materials in vivo, so as to achieve a simple test process, The effect of short test period and rapid evaluation of the safety of nanomaterials

Inactive Publication Date: 2011-04-20
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

However, due to the difference between in vivo and in vitro, the in vivo toxicity of materials canno

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[0026] Example 1

[0027] A method for testing the toxicity of CdSe quantum dot nanomaterials through a genetically modified zebrafish embryo model, the specific steps are:

[0028] (1) Healthy sexually mature fli1: EGFP strain zebrafish are placed in the incubator at a ratio of 1:1 to male and female. Embryos are obtained by mating between male and female fish in the early morning. Collection starts after the light is on for 0.5 hours. After the embryos are collected, they are washed. Then move it into the zebrafish embryo culture medium to remove the unfertilized eggs, the eggs with irregular division or the damaged egg membrane, and select the normally divided fertilized eggs for toxicity test; also include the following steps: add nanomaterial test solution while doing control Experiment, compare and observe the embryo development process, record embryo death, deformity, hatching and other indicators, and promptly remove dead embryos to avoid infection of other embryos and caus...

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Abstract

The invention relates to a method for testing the toxicity of a nanomaterial by using a transgenic zebra fish embryo model. The method comprises the following steps of: (1) adding nanomaterial test solution to be tested into zebra fish embryo culture solution which has been fertilized for 6 to 7 hours, or cultivating the mixture in an artificial climate cabinet to 72hpf or 96hpf; (2) taking the 72hpf or 96hpf embryo out, fixing the embryo with 4 volume percent paraformaldehyde for 24 to 30 hours, washing the embryo with phosphate (PBS) buffer solution for 2 to 4 times, and fixing the embryo on a glass slide for 24 to 48 hours, and observing blood vessels with a fluorescence microscope; and (3) judging whether the nanomaterial causes vascular injury or not according to an image acquired by the fluorescence microscope. The method has the advantages of readily available raw materials, simple administration manner and small dosage of the nanomaterial to be tested, simplicity, short test cycle and wide application prospect.

Description

【Technical field】 [0001] The invention relates to the technical field of nanomaterial toxicity testing, in particular to a method for testing nanomaterial toxicity through a transgenic zebrafish embryo model. 【Background technique】 [0002] Nanomaterials are new materials developed in the mid-1980s. They are different from microscopic atoms and molecules, and also different from the superconventional properties of macroscopic substances. They are often defined as granular particles with a size within 1nm to 100nm in a certain dimension , Layered materials, their size ranges between microcrystalline materials and atomic clusters. At present, the nanomaterials widely used at home and abroad mainly include carbon nanomaterials, semiconductor quantum dots, nano-oxides (titanium dioxide, silicon dioxide, zinc oxide, aluminum oxide) and nano-iron compounds. With the popularization of large-scale production and nano-products, the chances of occupational exposure and environmental ...

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Application Information

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IPC IPC(8): G01N33/00G01N21/64
Inventor 林匡飞孙雪张卫殷晓蕾苗优娜陆强蓝闽波刘莉莉赵宇侠徐圣友
Owner EAST CHINA UNIV OF SCI & TECH
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